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  4. Recombinant Mouse Interferon regulatory factor 7 (Irf7)

Recombinant Mouse Interferon regulatory factor 7 (Irf7)

  • 货号：
    CSB-YP011822MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP011822MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP011822MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP011822MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Irf7; Interferon regulatory factor 7; IRF-7
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-457
  • 氨基酸序列
    MAEVRGVQRV LFGDWLLGEV SSGQYEGLQW LNEARTVFRV PWKHFGRRDL DEEDAQIFKA WAVARGRWPP SGVNLPPPEA EAAERRERRG WKTNFRCALH STGRFILRQD NSGDPVDPHK VYELSRELGS TVGPATENRE EVSLSNALPT QGVSPGSFLA RENAGLQTPS PLLSSDAGDL LLQVLQYSHI LESESGADPV PPQAPGQEQD RVYEEPYAAW QVEAVPSPRP QQPALTERSL GFLDVTIMYK GRTVLQAVVG HPRCVFLYSP MAPAVRTSEP QPVIFPSPAE LPDQKQLHYT ETLLQHVSPG LQLELRGPSL WALRMGKCKV YWEVGSPMGT TGPSTPPQLL ERNRHTPIFD FSTFFRELEE FRARRRQGSP HYTIYLGFGQ DLSAGRPKEK TLILVKLEPW VCKAYLEGVQ REGVSSLDSS SLGLCLSSTN SLYEDIEHFL MDLGQWP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction via both the virus-activated, MyD88-independent pathway and the TLR-activated, MyD88-dependent pathway. Induces transcription of ubiquitin hydrolase USP25 mRNA in response to lipopolysaccharide (LPS) or viral infection in a type I IFN-dependent manner. Required during both the early and late phases of the IFN gene induction but is more critical for the late than for the early phase. Exists in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization where along with other coactivators it can activate transcription of the type I IFN and ISG genes. Can also play a role in regulating adaptive immune responses by inducing PSMB9/LMP2 expression, either directly or through induction of IRF1. Binds to the Q promoter (Qp) of EBV nuclear antigen 1 a (EBNA1) and may play a role in the regulation of EBV latency. Can activate distinct gene expression programs in macrophages and regulate the anti-tumor properties of primary macrophages.
  • 基因功能参考文献：
    1. IRF7 represents a novel regulator of granulocytic myeloid-derived suppressor cells development in cancer, which may have predictive value for tumor progression. PMID: 28092673
    2. a specific requirement for IRF7 in autoantibody production and uncovers a new layer of complexity in the pathogenesis of Systemic lupus erythematosus (SLE). PMID: 27527596
    3. the transcription factor NFATC3 binds to IRF7 and functions synergistically to enhance IRF7-mediated IFN expression in Plasmacytoid dendritic cells. PMID: 27697837
    4. Bcl6, by interacting with the co-factors NcoR2 and HDAC3, plays a pivotal role in controlling IRF7 induction and antiviral signaling priming. PMID: 26728228
    5. poly I:C augments IFN-gamma-induced NO production at the transcriptional level via enhanced IRF7 activation PMID: 25582076
    6. IRF7 protects against vascular smooth muscle cell proliferation. PMID: 25304854
    7. Data showed that expression of interferon regulatory factor 7 (IRF7) increased during the M2-like to M1-like switch in microglia in vitro and in vivo. PMID: 25422089
    8. Data suggest that Trim35 (tripartite motif-containing 35) down-regulates type I interferon production in dendritic cells via toll-like receptors 7/9 and down-regulates stability of Irf7 (interferon regulatory factor 7) via ubiquitination/proteasomes. PMID: 25907537
    9. These results establish a dominant protective role for MAVS, IRF-3 and IRF-7, and IFNAR in restricting Oropouche virus infection and tissue injury and suggest that IFN signaling in nonmyeloid cells contributes to the host defense. PMID: 25717109
    10. IRF7 suppresses antiparasitic immunity in the spleen, while IFNAR1-mediated, but IRF7-independent, signaling contributes to pathology in the brain during experimental blood-stage malaria. PMID: 25319247
    11. IRF-3, IRF-7, and IPS-1 promote host defense against acute human metapneumovirus infection in neonatal mice PMID: 24726644
    12. IRF7 acts as a novel negative regulator of pathological cardiac hypertrophy by inhibiting nuclear factor-kappaB signaling and may constitute a potential therapeutic target for pathological cardiac hypertrophy. PMID: 24396025
    13. the combined actions of IRF-3 and IRF-7 are necessary for efficient control of early DENV infection; and the late, IRF-3- and IRF-7-independent pathway contributes to anti-DENV immunity. PMID: 24043884
    14. These findings suggest that Nmi is a negative regulator of the virus-triggered induction of type I IFNs that targets IRF7 PMID: 23956435
    15. In addition to controlling viral replication, IRF-3 and -7 therefore play coordinating roles in modulation of inflammation during herpes simplex virus infection. PMID: 23777662
    16. Data suggest that Irf7 plays role in high-fat diet-induced changes in insulin sensitivity/inflammation leading to obesity/type 2 diabetes; Irf7 is up-regulated in visceral fat/liver/skeletal muscle in obesity; Irf7 knockout mice resist weight gain. PMID: 23695216
    17. Many genes suppressed in bone metastases are targets of Irf7. Restoration of Irf7 in tumor cells or administration of interferon led to reduced bone metastases & prolonged survival. PMID: 22820642
    18. IRF7 is required for the early innate control of lymphocytic choriomeningitis virus infection, likely through the regulation of the appropriate type I IFN response, but is not required for the antiviral CD8+ T cell-dependent clearance of the virus. PMID: 23490048
    19. OASL1 inhibits translation of the type I interferon-regulating transcription factor IRF7. PMID: 23416614
    20. studies suggest that expression of the IFN-alpha/beta receptor on macrophages/neutrophils and dendritic cells, as well as of IRF-3 and IRF-7, is critical for innate immune responses to NoV infection PMID: 23035219
    21. Pellino3 targets the IRF7 pathway and facilitates autoregulation of TLR3- and viral-induced expression of type I interferons. PMID: 23042151
    22. studies provide new insight into the role of IRF7 in regulating the development of adaptive immune responses and viral pathogenesis during an acute lymphocytic choriomeningitis infection PMID: 22875973
    23. Lactobacillus acidophilus induces IFN-beta signals independently of interferon regulatory factor (IRF)3 and IRF7 but is reduced in IRF3/7- and IRF1-deficient dendritic cells. PMID: 22896628
    24. data suggest that the FOXO3-IRF7 regulatory circuit represents a novel mechanism for establishing the requisite set points in the interferon pathway that balances the beneficial effects and deleterious sequelae of the antiviral response PMID: 22982991
    25. Chikungunya virus infection of adult mice deficient in interferon response factors 3 and 7 (IRF3/7(-/-)) is lethal. Mortality was associated with undetectable levels of alpha/beta interferon. PMID: 22761364
    26. Collectively, these data demonstrate that the early type I IFN response to lymphocytic choriomeningitis virus infection in the central nervous system is controlled by a concerted action of IRF3 and -7. PMID: 22514347
    27. IRF-3 & IRF-7 are functionally redundant in an age-dependent manner. Lack of both results in lethal Chikungunya infection in adult mice. PMID: 22371392
    28. Data suggest that IRF7 signaling is critical for regulation of inflammatory responses in the CNS. PMID: 22196084
    29. Irf7-mediated control of the Gbp2 gene required the presence and basal activity of the S/T kinase TANK-binding kinase 1. PMID: 22252317
    30. In this study the increase in Irf-7 mRNA within the background of reduced Dcp2 levels was attributed to a stabilization of the Irf-7 mRNA, suggesting that Dcp2 normally modulates Irf-7 mRNA stability. PMID: 22252322
    31. Our data therefore add interferon regulatory factor 7 (IRF-7) to the growing list of interferon regulatory factors that have effects on downstream events in the acquired cellular immune response to nonviral pathogens PMID: 21149596
    32. IRF7 upregulates activating transcription factor (ATF)4 activity and expression. PMID: 21148039
    33. analysis of IFN-stimulated response elements (ISREs) that bind to both the IFN-stimulated gene factor 3 (ISGF3) as well as to IFN response factor 7 (IRF7) PMID: 20943654
    34. interferon regulatory factor 7C has dual roles in Epstein-Barr virus-mediated lymphocyte transformation PMID: 20209099
    35. new role for IRF-7 as a regulator of innate microbicidal activity against Leishmania donovani PMID: 20300600
    36. transcription factor IRF-7 interacts with MyD88 to form a complex in the cytoplasm PMID: 15492225
    37. A comprehensive structure-activity examination of potential IRF7 phosphorylation sites located in the carboxyl-terminal regulatory domain are mapped to amino acids 437-438 and a redundant set of sites at either amino acids 429-431 or 441. PMID: 15743772
    38. there is a dual mechanism for the regulation of IFN production by differential expression of IRF7, involving positive feedback at local sites of infection combined with robust systemic production by IRF7-expressing plasmacytoid dendritic cells PMID: 15767254
    39. the transcription factor IRF-7 is essential for the induction of IFN-alpha/beta genes via the virus-activated, MyD88-independent pathway and the TLR-activated, MyD88-dependent pathway PMID: 15800576
    40. the spatiotemporal regulation of MyD88-IRF-7 signalling is critical for a high-level IFN induction in response to TLR9 activation PMID: 15815647
    41. upregulated during lymphocytic choriomeningitis virus infection, a process dependent on IFN-alpha/beta and STAT2 PMID: 15919906
    42. two distinct posttranslational mechanisms regulate IRF7 activity in response to viral infection, with protein turnover attenuating responses postinfection in most cell types while infection-induced protein stabilization PMID: 16472772
    43. Interferon (IFN) regulatory factor (IRF)7 is required for IFN-alpha and IFN-beta production. PMID: 17513736
    44. Anti-viral effect of thymosin alpha1 occurred through the activation of dendritic cells via the TLR9/MyD88, leading to activation of IFN regulatory factor 7 and the promotion of the IFN-alpha/IFN-gamma-dependent effector pathway PMID: 17804687
    45. findings highlight the role of 4E-BPs as negative regulators of type-I IFN production, via translational repression of Irf7 mRNA PMID: 18272964
    46. Irf7 induces the antiviral alpha interferon response and protects against lethal West Nile virus infection. PMID: 18562536
    47. SUMO modification and RIG-I activation are an integral part of IRF3 and IRF7 activity that contributes to postactivation attenuation of IFN production PMID: 18635538
    48. mammalian chromosomal DNA that accumulates in macrophages due to inefficient degradation activates genes in both IRF3/IRF7-dependent and -independent manners. PMID: 18991290
    49. The interaction between bICP0 and interferon regulatory factor 7 (IRF7) correlates with reduced trans-activation of the IFN-beta promoter by IRF7. PMID: 19176627
    50. IRF7-dependent antiviral immune responses were not essential for resistance against acute murine cytomegalovirus infection in vivo. PMID: 19283778

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Note=The phosphorylated and active form accumulates selectively in the nucleus.
  • 蛋白家族：
    IRF family
  • 数据库链接：

    KEGG: mmu:54123

    STRING: 10090.ENSMUSP00000026571

    UniGene: Mm.3233