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  4. Recombinant Mouse Interferon regulatory factor 4 (Irf4)

Recombinant Mouse Interferon regulatory factor 4 (Irf4)

  • 货号：
    CSB-YP011819MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP011819MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP011819MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP011819MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP011819MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Irf4; Spip; Interferon regulatory factor 4; IRF-4; Lymphocyte-specific interferon regulatory factor; LSIRF; NF-EM5; PU.1 interaction partner; Transcriptional activator PIP
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-450
  • 氨基酸序列
    MNLETGSRGS EFGMSAVSCG NGKLRQWLID QIDSGKYPGL VWENEEKSVF RIPWKHAGKQ DYNREEDAAL FKAWALFKGK FREGIDKPDP PTWKTRLRCA LNKSNDFEEL VERSQLDISD PYKVYRIVPE GAKKGAKQLT LDDTQMAMGH PYPMTAPYGS LPAQQVHNYM MPPHDRSWRD YAPDQSHPEI PYQCPVTFGP RGHHWQGPSC ENGCQVTGTF YACAPPESQA PGIPIEPSIR SAEALALSDC RLHICLYYRD ILVKELTTTS PEGCRISHGH TYDVSNLDQV LFPYPDDNGQ RKNIEKLLSH LERGLVLWMA PDGLYAKRLC QSRIYWDGPL ALCSDRPNKL ERDQTCKLFD TQQFLSELQV FAHHGRPAPR FQVTLCFGEE FPDPQRQRKL ITAHVEPLLA RQLYYFAQQN TGHFLRGYEL PEHVTTPDYH RSLRHSSIQE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes.
  • 基因功能参考文献：
    1. MBD2 Regulates Th17 Cell Differentiation and Experimental Severe Asthma by Affecting IRF4 Expression PMID: 28808358
    2. study identifies IRF4 as central regulator of TH1 responses and cellular metabolism. We propose that this function of IRF4 is fundamental for the initiation and maintenance of all TH cell responses. PMID: 27762344
    3. Nur77 suppresses CD4(+) T cell proliferation and uncover a suppressive role for Irf4 in TH2 polarization; halving Irf4 gene-dosage leads to increases in GATA3(+) and IL-4(+) cells. PMID: 28538176
    4. Muramyl dipeptide reduces fat inflammation and liver insulin resistance via NOD2. NOD1-activating muropeptides exacerbate glucose intolerance. IRF4 dictates insulin-sensitizing effects of NOD2, but not NOD1, muropeptides. PMID: 28434881
    5. Young mice had higher levels of IRF4 in the ischemic brains, suggesting that aging has a significant influence on stroke outcomes in mice, which is probably mediated by age-specific inflammatory responses. PMID: 28905935
    6. IRF4 is highly induced in graft-infiltrating T cells and is required for heart transplant rejection. PMID: 29221730
    7. The findings indicate that IRF4 functions as a central node in a TCR-responsive transcriptional circuit that establishes and sustains T cell exhaustion during chronic viral infection. PMID: 29246443
    8. RHS6 is a critical regulatory element for allergic airway inflammation and for coordinate regulation of Th2 cytokine genes by recruiting GATA3, SATB1, and IRF4. PMID: 27878828
    9. this study shows that epicutaneous sensitization to house dust mite allergen requires interferon regulatory factor 4-dependent dermal dendritic cells PMID: 28189772
    10. Cell fate and metabolic state are linked by transcriptional regulators, such as IRF4 and FoxO1, with dual roles in lineage and metabolic choice. Instructing some cells to utilize nutrients for anabolism and differentiation while other cells catabolically self-digest and self-renew may enable growth and repair in metazoa. PMID: 28009285
    11. Here the s show that concomitant loss of Tet2 and Tet3 in mice at early B cell stage blocked the pro- to pre-B cell transition in the bone marrow, decreased Irf4 expression and impaired the germline transcription and rearrangement of the Igkappa locus. PMID: 27869616
    12. IRF4 protects arteries against neointima formation by promoting the expression of KLF4 by directly binding to its promoter. PMID: 28851732
    13. Data show that Fos-Related Antigen-2 (Fra-2) is a key upstream regulator of forkhead box O1 (Foxo1) and interferon regulatory factor 4 (Irf4) expression and influences proliferation and differentiation of B cells at multiple stages. PMID: 28566276
    14. GM-CSF can mediate inflammation and pain by regulating IRF4-induced CCL17 production PMID: 27525438
    15. MTORC2 operated in parallel with the IL-4Ralpha-Stat6 pathway. PMID: 27760338
    16. IRF4 mRNA and protein expression was remarkably suppressed during the development of myeloid-derived suppressor cells in the tumor microenvironment. IRF4 expression in MDSCs can modulate their activity to inhibit T cell proliferation through IL-10 production and ROS generation, and myeloid-specific deletion of IRF4 leads to the increase of MDSC differentiation. IRF4 reduction induced by tumors can increase MDSC numbers. PMID: 27601624
    17. propose that Irf4 is imperative for thymic Treg homeostasis because it regulates thymic epithelial cell-specific expression of several chemokines and costimulatory molecules indicated in thymocyte development and Treg induction PMID: 28108558
    18. MHC II(+) resident peritoneal and pleural macrophages are continuously replenished by blood monocytes recruited to the peritoneal and pleural cavities constitutively, starting after birth, where they require IRF4 and signals likely derived from the microbiome to fully differentiate. PMID: 27551152
    19. this study shows that PU.1 functions as a positive regulator of CD11c gene expression by directly binding to the Itgax promoter and through transactivation of the Irf4 gene PMID: 28338898
    20. this study shows that the affinity of different enhancers for the BATF-IRF4 complex might underlie divergent signaling outcomes in response to various strengths of TCR signaling PMID: 28346410
    21. this study shows that baicalin upregulates IRF4 protein expression and alleviates colitis by modulating macrophage polarization to the M2 phenotype PMID: 27039210
    22. data reveal that CCR7 plays multifaceted roles in regulating collecting vessel permeability and fibrosis, with one of the key players being IRF4-dependent DCs. PMID: 26999610
    23. IRF4 was essential for the survival of plasma cells. PMID: 26779600
    24. a critical role for IRF4 in regulating protective anti-viral CD8+ T cell responses by ensuring a balanced ratio of T-bet to Eomes PMID: 26714260
    25. variation in IRF4 or IRF8 levels resulting from genetic polymorphisms or environmental cues will govern tissue dendritic cells numbers and regulate the magnitude of their functional responses. PMID: 26746189
    26. DC dysfunction in lupus-prone mice relies on both IRF4-dependent and IRF4-independent pathways PMID: 26544714
    27. APN can help to reduce periodontitis-related bone loss, modulate JMJD3 and IRF4 expression, and macrophage infiltration. PMID: 26399931
    28. Data indicate that interferon regulatory factors IRF4 and IRF8 control distinct activated B cell states. PMID: 26437243
    29. this study determined the small angle x-ray scattering solution structure of full-length IRF4, which, together with circular dichroism studies, suggests that the linker region is not extended but folds into a domain structure PMID: 26405037
    30. data support a novel and critical role for Nr4a1 in the regulation of CD8(+) T cell expansion and effector function through transcriptional repression of Irf4. PMID: 26363057
    31. inhibition of IRF4 attenuates acute liver allograft rejection in mice, and this is associated with promoted M2 macrophage differentiation. PMID: 26095713
    32. NOD2 downregulates colonic inflammation by IRF4-mediated inhibition of K63-linked polyubiquitination of RICK and TRAF6. PMID: 24670424
    33. The transcription factors IRF4 and BATF are major regulators of antiviral cytotoxic T-cell immunity. PMID: 24531538
    34. miR-125b induces myeloid and B-cell leukemia by inhibiting IRF4 through distinct mechanisms, inducing myeloid leukemia through repressing IRF4 at the mRNA level without altering genomic DNA and B-cell leukemia via deletion of the gene encoding IRF4 PMID: 25006123
    35. we have shown that the IRF4-SRF axis is a novel signalling pathway critical for neuronal survival in the setting of ischaemic stroke. PMID: 24510125
    36. both IRF3 and IRF4 are induced by adiponectin, with adiponectin-AMPK and adiponectin-PPARalpha signalling regulating IRF3 and IRF4, respectively. PMID: 24627105
    37. Murine gammaherpesvirus 68 reactivation from B cells requires IRF4 but not XBP1. PMID: 25078688
    38. Study shows that IRF4 is transcriptionally regulated by cold and cAMP in murine and human brown adipose tissue (BAT) prior to the induction of Ucp1 and that mice that overexpress IRF4 transgenically in BAT display enhanced thermogenic gene expression, energy expenditure, and cold tolerance. PMID: 24995979
    39. IRF4 is a key component that translates the strength of TCR signaling into a graded response of virus-specific CD8(+) T cells. PMID: 24835398
    40. The BTB-ZF transcription factor Zbtb20 is driven by Irf4 to promote plasma cell differentiation and longevity. PMID: 24711583
    41. IRF4 expression is upregulated during osteoclast differentiation induced by RANKL. PMID: 24039733
    42. Data indicate that transcription factor IFN regulatory factor 4 (IRF4) was required for the development of early germinal centers (GCs) B cells. PMID: 24591370
    43. These results imply that IRF4 expression in DCs inhibits their ability to produce IL-12 while promoting their migratory behavior, thus regulating CD4(+) T cell responses against local infection with L. major. PMID: 24489086
    44. IRF4 functions as a specific regulatory determinant for CD11b+ DC subsets and programs the enhanced expression of genes required for MHC class II antigen presentation, thereby enabling such cells to more efficiently prime helper T cell responses in vivo. PMID: 24362890
    45. T-bet negatively regulates Th17 lineage commitment via direct repression of IRF4. PMID: 24249732
    46. IRF4 controls the positioning of mature B cells in the lymphoid microenvironments by regulating NOTCH2 expression PMID: 24323359
    47. IRF4 was vital for sustaining the expansion & effector differentiation of T8 cells. It promoted Blimp1 & T-bet expression & function, & repressed cell-cycle-arrest & apoptosis genes. Loss impaired antiviral immunity. PMID: 24211184
    48. IRF4 is a novel regulator in the pathogenesis of chronic lymphocytic leukemia PMID: 23897826
    49. IRF4 is crucial for the protective CD8+ T-cell response to L. monocytogenes. IRF4-deficient mice could not clear L. monocytogenes infection and generated decreased numbers of L. monocytogenes-specific CD8+ T cells with impaired phenotype and function. PMID: 23980171
    50. The transcription factor IRF4 is essential for TCR affinity-mediated metabolic programming and clonal expansion of T cells. PMID: 24056747

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    IRF family
  • 组织特异性：
    Lymphoid cells.
  • 数据库链接：

    KEGG: mmu:16364

    STRING: 10090.ENSMUSP00000021784

    UniGene: Mm.4677