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  4. Recombinant Mouse Interferon-induced protein with tetratricopeptide repeats 1 (Ifit1)

Recombinant Mouse Interferon-induced protein with tetratricopeptide repeats 1 (Ifit1)

  • 货号：
    CSB-YP011018MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP011018MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP011018MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP011018MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP011018MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Ifit1
  • Uniprot No.：
  • 别名：
    Ifit1; Garg16; Ifi56; Isg56Interferon-induced protein with tetratricopeptide repeats 1; IFIT-1; Glucocorticoid-attenuated response gene 16 protein; GARG-16; Interferon-induced 56 kDa protein; IFI-56K; P56
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-463
  • 氨基酸序列
    MGENADGDQV MENLLQLRCH FTWKLLFENN DIPDLEVRIS EQVQFLDIKN PLGMHNLLAY VRHLKGQQDE ALQSLKEAEA LIQSEQLSKR SLATWGNCAW LHYHRGSLAE AQIYLDKVEK VCKEFSSPFR YRLECAEMDC EEGWALLKCG GGNYKQAMAC FAKALKVEPE NPEYNTGYAV VAYRQDLDDN FISLEPLRKA VRLNPEDPYL KVLLALKLQD LGEHVEAEAH IEEALSSTSC QSYVIRYAAK YFRRKHRVDK ALHLLNRALQ ASPSSGYLHY QKGLCYKQQI SQLRTSRNRQ PRRQDNVQEL AQQAIHEFQE TLKLRPTFEM AYVCMAEVQA EIHQYEEAER NFQKALNNKT LVAHIEQDIH LRYGRFLQFH KQSEDKAITL YLKGLKVEEK SFAWRKLLTA LEKVAERRVC QNVHLVESTS LLGLVYKLKG QEKNALFYYE KALRLTGEMN PAF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Interferon-induced antiviral RNA-binding protein that specifically binds single-stranded RNA bearing a 5'-triphosphate group (PPP-RNA), thereby acting as a sensor of viral single-stranded RNAs and inhibiting expression of viral messenger RNAs. Single-stranded PPP-RNAs, which lack 2'-O-methylation of the 5' cap and bear a 5'-triphosphate group instead, are specific from viruses, providing a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Directly binds PPP-RNA in a non-sequence-specific manner. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism).
  • 基因功能参考文献：
    1. ISG20 acts as an indirect regulator of RNA virus replication in the cytoplasm through the upregulation of many other ISGs. PMID: 30232164
    2. IFIT1 expression is associated with podocytes damage, and capable of suppressing some proteins essential to glomerular filtration. PMID: 27823966
    3. Human IFIT1 and mouse IFIT1 have divergent antiviral specificities: only IFIT1 proteins inhibit a virus encoding a cap 2'O-methyltransferase. PMID: 27240734
    4. Data suggest that, with up-regulation of IFIT1, alphaviruses (Venezuelan equine encephalitis virus; Chikungunya virus) with wild-type 5' untranslated regions are inducers of type I IFN (interferon), suggesting a new mechanism of type I IFN induction. PMID: 25927359
    5. Targeted expression of Ifit1 in hepatocytes protects mice from lipopolysaccharide and D-galactosamine induced lethal hepatitis. PMID: 26459629
    6. IFIT1 is not a dominant restriction factor against three different families of negative-sense RNA viruses. PMID: 26157117
    7. The specificity of IFIT1 for 2'O-unmethylated RNA serves as potent antiviral mechanism against viruses lacking 2'O-methyltransferase activity and at the same time allows unperturbed progression of the antiviral program in infected cells. PMID: 24098121
    8. Thus, Ifit1 inhibits replication of MTase-defective Japanese encephalitis virus by inhibiting mRNA translation through direct binding to mRNA 5' structures. PMID: 23824812
    9. Study identified Ifit1 as an important innate immune bottleneck. PMID: 22745654
    10. results suggest that virulent strains of West Nile virus have largely evaded the antiviral effects of Ifit1, and viral mutants lacking 2'-O methylation are controlled in vivo by Ifit1-dependent and -independent mechanisms in different cell types PMID: 22589727
    11. IFIT1 bound PPP-RNA antagonizes viruses by sequestering specific viral nucleic acids. PMID: 21642987
    12. results demonstrate that the 2'-O methylation of the 5' cap of viral RNA functions to subvert innate host antiviral responses through escape of IFIT-mediated suppression PMID: 21085181
    13. block different functions of eIF3 by binding to its different subunits. PMID: 15561726
    14. Expression increased significantly during LCMV and WNV infection, being widespread and localized predominantly to common as well as distinct neuronal populations in the brain. PMID: 17079283
    15. Our findings provide the first evidence of a differential pattern of expression of ISG54 and ISG56 genes by IFN-alpha and IFN-beta. PMID: 17553874

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    IFIT family
  • 数据库链接：

    KEGG: mmu:15957

    STRING: 10090.ENSMUSP00000099888

    UniGene: Mm.439751