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  4. Recombinant Mouse Integrin-linked protein kinase (Ilk)

Recombinant Mouse Integrin-linked protein kinase (Ilk)

  • 货号：
    CSB-YP011685MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP011685MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP011685MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP011685MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP011685MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Ilk; Integrin-linked protein kinase; EC 2.7.11.1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-452
  • 氨基酸序列
    MDDIFTQCRE GNAVAVRLWL DNTENDLNQG DDHGFSPLHW ACREGRSAVV EMLIMRGARI NVMNRGDDTP LHLAASHGHR DIVQKLLQYK ADINAVNEHG NVPLHYACFW GQDQVAEDLV ANGALVSICN KYGEMPVDKA KAPLRELLRE RAEKMGQNLN RIPYKDTFWK GTTRTRPRNG TLNKHSGIDF KQLNFLAKLN ENHSGELWKG RWQGNDIVVK VLKVRDWSTR KSRDFNEECP RLRIFSHPNV LPVLGACQAP PAPHPTLITH WMPYGSLYNV LHEGTNFVVD QSQAVKFALD MARGMAFLHT LEPLIPRHAL NSRSVMIDED MTARISMADV KFSFQCPGRM YAPAWVAPEA LQKKPEDTNR RSADMWSFAV LLWELVTREV PFADLSNMEI GMKVALEGLR PTIPPGISPH VCKLMKICMN EDPAKRPKFD MIVPILEKMQ DK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Regulates cell motility by forming a complex with PARVB. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.
  • 基因功能参考文献：
    1. Depletion of integrin-linked kinase (ILK) in the pancreatic epithelial cells results in glucose intolerance due to a loss of the intra-islet vasculature. In turn, blood vessels accumulate at the islet periphery. PMID: 27995929
    2. these data demonstrate the importance of integrin-mediated cell-matrix interactions and ILK signaling in osteoprogenitors in the control of osteoblast functioning during juvenile bone mass acquisition and adult bone remodeling and homeostasis. PMID: 28574598
    3. We conclude that ILK is critical for maintaining the collecting duct epithelium and renal function and is a key intermediate for periostin activation of signaling pathways involved in cyst growth and fibrosis in PKD. PMID: 28522687
    4. Ilk and ELMO2 modulate recycling endosomes in keratinocytes undergoing intercellular adhesion mediated through cell-cell contacts, including E-cadherin-based adherens junctions. PMID: 27627840
    5. Ilk regulates cellular mechanics facilitating the motility in 3D extracellular matrices. PMID: 28011283
    6. It was concluded that ILK depletion modifies the transcription of GLUT4, which results in reduced peripheral insulin sensitivity and glucose uptake, suggesting ILK as a molecular target and a prognostic biomarker of insulin resistance. PMID: 28490443
    7. results demonstrate that TGF-beta1-induced autophagy links beta-catenin and Smad signaling to promote epithelial-mesenchymal transition in C1.1 cells through a novel pY654-beta-catenin/p-Smad2/ILK pathway. PMID: 27177845
    8. ILK deletion impaired the developmental profile, proliferation, and differentiation of oligodendrocyte precursor cells. PMID: 28077718
    9. Our results define ILK as a key mechanotransducer in modulating breast cancer stem-like cells development in response to tissue mechanics and oxygen tension. PMID: 27503933
    10. iNOS-derived NO plays a crucial role during atherosclerosis by regulating the endocytic-lysosomal degradation of ILK in endothelial cells. PMID: 28546219
    11. Hepatic ILK deletion has no effect on insulin action in lean mice but sensitizes the liver to insulin during the challenge of high fat feeding. This effect corresponds to changes in the expression and activation of key insulin signaling pathways as well as a greater capacity for hepatic mitochondrial glucose oxidation. PMID: 27899483
    12. this study has identified a new role for Parvin and alphaPix downstream of the integrin-ILK signaling axis for mammary epithelial cell differentiation. PMID: 27019299
    13. Work provides a new tool for studying mouse oligodendrocyte precursor cell migration and highlights the role of integrin-linked kinase in its regulation on extracellular matrix proteins relevant to multiple sclerosis. PMID: 26831726
    14. We conclude that ILK negatively and independently of PI3K regulated MEF2C phosphorylation activity and MCK mRNA expression in C2C12 cells PMID: 26788505
    15. high extracellular concentration of phosphate induced senescence in cultured smooth muscle through the activation of IGF-1 receptor and ILK overexpression PMID: 26467393
    16. This study demonstrates a role for ILK in regulating laminin2-mediated adhesion and Ln-2-independent growth cone size/activity in oligodendrocyte PMID: 26614167
    17. ILK is indispensable for Brain-derived neurotrophic factor-mediated hippocampal neurogenesis and memory enhancement upon Environment enrichment stimuli via regulating glycogen synthase kinase-3beta activity. PMID: 26095336
    18. ELMO2-RhoG-ILK complex has a key role in integrin-independent stabilization of the microtubule network in differentiated keratinocytes. PMID: 25995380
    19. ILK is a key regulator of the bulge extracellular matrix microenvironment, thereby governing the activation and maintenance of hair follicle stem cells. PMID: 26349061
    20. CTGF overexpression induces epithelial to mesenchymal transition in mouse primary AT II cells, which is mediated by ILK PMID: 25580742
    21. These data demonstrate that ILK is a linker between Integrin receptors and the Dynactin complex to regulate mitotic spindle orientation. PMID: 25669897
    22. These observations demonstrate an important role for beta1-integrin/ILK signaling in regulating astrogliosis from ESCs and suggest ILK as a potential target for limiting glial scar formation after nervous system injury. PMID: 25098415
    23. Cardiomyocyte ILK deletion produces a lethal arrhythmogenic cardiomyopathy associated with important ion channel and structural remodeling. PMID: 26071395
    24. ILK mediates the accumulation of integrin-associated proteins during podosome maturation and stability for efficient degradation of the subjacent ECM during the invasive migration of DCs. PMID: 24508783
    25. ILK mediates cardiomyocyte force transduction through regulation of SERCA-2a and phosphorylation of phospholamban. PMID: 25208486
    26. Integrin-linked kinase (ILK) functions in fibroblasts to direct TbetaRII away from degradative pathways during their differentiation into myofibroblasts. PMID: 25268583
    27. findings of this study indicate that, although ILK is not essential for platelet activation, it plays a critical role in facilitating rapid platelet activation, which is essential for stable thrombus formation PMID: 24888521
    28. integrin-linked kinase and Rac1 mediate Staphylococcus aureus keratinocyte invasion PMID: 25416549
    29. In response to Helicobacter pylori infection, ILK modulates TNF-alpha production and activates nuclear factor kappaB signaling via p65 Ser-536 phosphorylation. PMID: 25100717
    30. ILK regulates hair bud cell polarity and functions upstream from laminin-511 assembly to regulate the developmental progression of hair follicles beyond the germ stage. PMID: 24371086
    31. ILK regulates expression of AQP2 and its presence in renal tubule apical membrane. Polyuria and decreased urine osmolality were present in ILK conditional-knockdown adult mice. AQP2 and ILK colocalize in cultured inner medullary collecting duct cells. PMID: 24784577
    32. These findings indicate that wild type ILK and the non-oncogenic ILK(R211A) mutation comprise a cardioprotective module with Hsp/c70. PMID: 24260102
    33. ILK is required for complete activation of signalling cascades downstream of the FGF receptor in lens epithelium and fibre cells during development and thus is involved in epithelial proliferation, survival and subsequent fibre differentiation. PMID: 24472646
    34. Deletion of ILK in Neural crest cells resulted in abnormal Neural crest cells migration to the pharyngeal arches and the outflow tract and impaired smooth muscle differentiation. PMID: 24131868
    35. Cardiomyocyte-specific integrin-linked kinase deletion leads to a lethal cardiomyopathy characterized by cardiomyocyte death, fibrosis, and inflammation. PMID: 24319095
    36. Integrin-linked kinase plays a key role in the regulation of angiotensin II-induced renal inflammation. PMID: 24383472
    37. In neural crest-derived cells, ILK and Pinch1 act as cytoplasmic effectors of beta1 integrin in a pathway that protects against aneurysms. PMID: 23744273
    38. Cilium-associated ILK interacts with Smo and beta-arrestins. PMID: 23877428
    39. effect of ILK deletion on the regulation of vascular tone through the soluble guanylate cyclase/protein kinase G pathway PMID: 23715557
    40. propose that in vivo, functional redundancy is able to compensate for the loss of ILK activity, leading to the absence of an obvious phenotype when osteoblast-specific Ilk-deficient mice reach puberty PMID: 24095779
    41. introducing a mutation in the paxillin-binding site of integrin-linked kinase into the germ line of mice (ILK-VT/GG) caused vasculogenesis defects, resulting in a general developmental delay and death at around embryonic day 12.5 PMID: 23658024
    42. CHIP and Hsp90 axis regulates ILK turnover in fibroblasts. PMID: 23612611
    43. ILK is required as a scaffold to allow actin-microtubule interactions and correct positioning of caveolin 1 close to the plasma membrane. PMID: 23404105
    44. ILK is needed to polarize microtubule plus ends at the basolateral membrane and disrupting each of these components prevents mammary gland lumen formation. PMID: 23263281
    45. Data show the essential role of integrin-linked kinase in regulation of phagocytosis in keratinocytes. PMID: 22767228
    46. Tissue plasminogen activator activates the NF-kappaB pathway in macrophages through a signaling pathway involving annexin A2/CD11b-mediated integrin-linked kinase. PMID: 22677557
    47. Down-regulated miR-625 suppresses invasion and metastasis of gastric cancer by targeting ILK PMID: 22677169
    48. involvement of ILK not only in epidermal differentiation and barrier formation, but also in melanocyte genesis and function PMID: 22574216
    49. ILK siRNA inhibits tumor growth and lung metastasis in mouse xenograft model. PMID: 22570869
    50. CTGF and ILK were involved in high glucose-induced phenotypic alterations of podocytes. ILK acted as a downstream kinase of CTGF and high glucose-induced ILK expression might occur through CTGF-dependent and -independent pathways. PMID: 21913214

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  • 亚细胞定位：
    Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, myofibril, sarcomere. Cell projection, lamellipodium.
  • 蛋白家族：
    Protein kinase superfamily, TKL Ser/Thr protein kinase family
  • 组织特异性：
    Highly expressed in lung, heart, kidney, liver, brain, spleen and skeletal muscle. Weakly expressed in testis.
  • 数据库链接：

    KEGG: mmu:16202

    STRING: 10090.ENSMUSP00000033182

    UniGene: Mm.274846