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  4. Recombinant Mouse Hormone-sensitive lipase (Lipe)

Recombinant Mouse Hormone-sensitive lipase (Lipe)

  • 货号：
    CSB-YP012975MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP012975MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP012975MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP012975MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP012975MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Lipe; Hormone-sensitive lipase; HSL; EC 3.1.1.79
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-759
  • 氨基酸序列
    MDLRTMTQSL VTLAEDNMAF FSSQGPGETA RRLSNVFAGV REQALGLEPT LGQLLGVAHH FDLDTETPAN GYRSLVHTAR CCLAHLLHKS RYVASNRKSI FFRASHNLAE LEAYLAALTQ LRAMAYYAQR LLTINRPGVL FFEGDEGLTA DFLQEYVTLH KGCFYGRCLG FQFTPAIRPF LQTLSIGLVS FGEHYKRNET GLSVTASSLF TGGRFAIDPE LRGAEFERII QNLDVHFWKA FWNITEIEVL SSLANMASTT VRVSRLLSLP PEAFEMPLTS DPRLTVTISP PLAHTGPAPV LARLISYDLR EGQDSKVLNS LAKSEGPRLE LRPRPHQAPR SRALVVHIHG GGFVAQTSKS HEPYLKNWAQ ELGVPIFSID YSLAPEAPFP RALEECFFAY CWAVKHCDLL GSTGERICLA GDSAGGNLCI TVSLRAAAYG VRVPDGIMAA YPVTTLQSSA SPSRLLSLMD PLLPLSVLSK CVSAYSGTEA EDHFDSDQKA LGVMGLVQRD TSLFLRDLRL GASSWLNSFL ELSGRKPQKT TSPTAESVRP TESMRRSVSE AALAQPEGLL GTDTLKKLTI KDLSNSEPSD SPEMSQSMET LGPSTPSDVN FFLRPGNSQE EAEAKDEVRP MDGVPRVRAA FPEGFHPRRS SQGVLHMPLY TSPIVKNPFM SPLLAPDSML KTLPPVHLVA CALDPMLDDS VMFARRLRDL GQPVTLKVVE DLPHGFLSLA ALCRETRQAT EFCVQRIRLI LTPPAAPLN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Lipase with broad substrate specificity, catalyzing the hydrolysis of triacylglycerols (TAGs), diacylglycerols (DAGs), monoacylglycerols (MAGs), cholesteryl esters and retinyl esters. Shows a preferential hydrolysis of DAGs over TAGs and MAGs and of the fatty acid (FA) esters at the sn-1 and sn-2 positions of the glycerol backbone in TAGs. Preferentially hydrolyzes FA esters at the sn-3 position of the glycerol backbone in DAGs. Catalyzes the hydrolysis of 2-arachidonoylglycerol, an endocannabinoid and of 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.
  • 基因功能参考文献：
    1. HSL-deficient mice revealed a complex interorgan interaction between adipose tissue and liver: the role of HSL in the liver is minimal but adipose tissue deficiency of HSL can cause age-dependent hepatic steatosis PMID: 29232702
    2. Cardiac PLIN2 plays an important pathophysiological role in the development of dynamic steatosis and that the latter was prevented by upregulation of intracellular lipases, including Hormone-sensitive Lipase. PMID: 28851734
    3. Enhanced lipolysis in response to mitochondrial uncoupling relies on a form of autophagy as lipid droplets are captured by endolysosomal vesicles which is HSL/ATGL-independent. PMID: 28488768
    4. HSL ablation alters the density of lipid-raft microdomains in mouse testis. HSL-/- lipid rafts had less desmosterol and T-MAS; non-raft had more cholesterol.Monounsaturated acyl phospholipids were higher in raft than in non-raft fractions.HSL ablation reduced PUFA-phospholipids in both raft and non-raft fractions. PMID: 27355565
    5. ase. Similar changes of GPNMB and G0S2 expression were present in a human liposarcoma database. These results show that a previously-unknown, fully penetrant epistatic interaction between Pnpla2 and Lipe can cause liposarcoma in mice. DAKO mice provide a promising model for studying early premalignant changes that lead to late-onset malignant disease. PMID: 28459858
    6. Activities of adipose triglyceride lipase (ATGL), hormone sensitive lipolitic enzyme (HSL) and monoacylglycerol lipase (MGL) were significantly higher (51 %, 38 %, 49 %) in the DE group than the HF group (p < 0.05). MGL, there were no differences between the CO group, HF group, and DC group, with the DE group (70 %) being significantly higher (p < 0.05). PMID: 27596982
    7. Results clearly indicate that SF-1 is involved in the regulation of LIPE expression after activation of protein kinase A in adrenocortical cells. PMID: 26122391
    8. A role for HSL in kidney lipolysis:fasting up-regulates HSL levels and phosphorylation in mouse kidney. PMID: 25879679
    9. Data suggest that cardiotrophin-1 up-regulates lipolysis in white adipocytes via 1) induction of perilipin, 2) activation of HSL (via phosphorylation by PKA), and 3) inactivation of adipose triglyceride lipase (via up-regulation of inhibitor G0S2). PMID: 25351614
    10. QRFP-43 attenuates lipolysis by preventing the formation of an active complex between perilipin A, caveolin-1, the catalytic subunit of protein kinase and hormone-sensitive lipase on lipid droplets. PMID: 25677823
    11. PRIP promotes the translocation of phosphatases to lipid droplets to trigger the dephosphorylation of HSL and perilipin A, thus reducing PKA-mediated lipolysis. PMID: 24945349
    12. Whereas the catalytic function of HSL is necessary for spermatogenesis in mice, the presence of the N-terminal testis-specific fragment is not essential. PMID: 24797631
    13. Changes in fatty acid metabolism observed in testes from HSL-knockout male mice may underlie the infertility observed in these animals. PMID: 23369366
    14. HSL acts to drive cAMP/PKA-mediated regulation of StAR expression and steroidogenesis in mouse Leydig cells. PMID: 23362264
    15. High-fat diet strongly reduced HSL phosphorylation at Ser660 in mouse skeletal muscle. PMID: 23471217
    16. analysis ofsubstrate and stereo/regioselectivity of adipose triglyceride lipase, hormone-sensitive lipase, and diacylglycerol-O-acyltransferases PMID: 23066022
    17. Loss of hormone sensitive lipase is associated with Cholesteryl ester accumulation and accelerated cholesterol absorption in intestine. PMID: 22842588
    18. Resveratrol increased adipose triglyceride lipase gene and protein expressions, an effect that was not observed for hormone-sensitive lipase in mice 3T3-L1 adipocytes. PMID: 21543206
    19. the dissociation between cardiac steatosis and functional sequelae observed in HSL knockout mice suggests that excess fatty acid influx, rather than steatosis appears to play an important role in the pathogenesis of cardiac lipotoxicity PMID: 19706782
    20. functional roles of adipose triglyceride lipase and hormone-sensitive lipase in TNF-alpha-induced lipolysis PMID: 21969372
    21. Study illustrates the importance of HSL for normal lipid metabolism in response to a high fat diet. PMID: 21738729
    22. The absence of HSL directs cells within the bone marrow toward osteoblast differentiation and favors the maintenance of bone density with aging. PMID: 21566206
    23. findings show that the inhibition of lipolysis through genetic ablation of adipose triglyceride lipase or hormone-sensitive lipase ameliorates certain features of cancer-associated cachexia PMID: 21680814
    24. HSL modulates adipose metabolism is by providing intrinsic ligands or pro-ligands for PPARgamma. PMID: 20950707
    25. Fatty acid synthase and hormone-sensitive lipase expression in liver are involved in zinc-alpha2-glycoprotein-induced body fat loss in obese mice. PMID: 21180279
    26. Elevated B56alpha/PP2A inhibits HSL and lipolysis in white adipose tissue of DIO mice. PMID: 20534721
    27. The interaction of hormone-sensitive lipase with vimentin, and its hormonal dependence, was confirmed by coimmunoprecipitation. PMID: 20143880
    28. Data suggest that upregulation of adipose triglyceride lipase and suppression of hormone-sensitive lipase and AMP kinase signaling mediate high-fat diet-induced alterations in lipolysis and lipid utilization in adipocytes. PMID: 20107043
    29. Data demonstrate that HSL can interact with an N-terminal region located between amino acids 141 and 200 of Plin A as well as with a C-terminal region located between amino acids 406 and 480. PMID: 19515989
    30. Results identify a link between hormone-sensitive lipase, and suggest that disturbances in polyamine metabolism may affect insulin sensitivity. PMID: 19785415
    31. perilipin expression and phosphorylation state are critical regulators of lipid storage and hydrolysis in ACS1/FATP1 cells PMID: 11751901
    32. deficiency changes the plasma lipid profile by affecting the tissue-specific expression pattern of lipoprotein lipase in adipose tissue and muscle PMID: 11809748
    33. Hormone-sensitive lipase-deficient mice accumulate lipid droplets in such a way as to impair acute ACTH stimulation of corticosterone secretion PMID: 12193545
    34. function, structure and genetics of this enzyme in the intestinal mucosa PMID: 12482847
    35. hormone-sensitive lipase translocation requires the phosphorylation of both HSL and perilipin PMID: 12832420
    36. HSL has a role in insulin sensitivity in multiple target tissues of the hormone PMID: 12835327
    37. increased atherosclerosis in hormone-sensitive lipase transgenic mice appears to be due to the coupling of the efficient re-esterification of excess free cholesterol to its limited removal mediated by the cholesterol acceptors in these mice PMID: 14643795
    38. HSL is critically involved in the intracellular processing and availability of cholesterol for adrenal steroidogenesis PMID: 14657254
    39. HSL has roles in adipogenesis as well as in feeding behavior PMID: 14752112
    40. Availability of endogenous free fatty acids through HSL and an additional enzyme(s) is involved in providing lipid moieties for beta-cell signaling for secretion in response to glucose. PMID: 15220197
    41. hormone-sensitive lipase HSLtes has a role in male infertility in mice PMID: 15292223
    42. results suggest that in the absence of HSL, a metabolic switch from reliance on lipid to carbohydrate energy substrates takes place, supporting an important role of HSL in soleus muscle lipid metabolism PMID: 16199803
    43. Perilipin targets a novel pool of lipid droplets for lipolytic attack by hormone-sensitive lipase PMID: 16243839
    44. Beta-cell isoform of HSL is involved in maintaining lipid homeostasis in islets and contributes to the proper control of glucose stimulated insulin secretion. PMID: 16765472
    45. HSL occupies a vital role in adrenal steroidogenesis because of its link to utilization of selectively delivered cholesteryl esters from lipoproteins. PMID: 16985254
    46. These results indicate that the HSL gene is transcriptionally regulated by PPARgamma/RXRalpha heterodimer, and suggest that a cis-acting element regulates the HSL gene expression. PMID: 17134676
    47. Hormone-sensitive lipase-mediated lipolysis and subsequent free fatty acid signaling are components of adipose tissue remodeling following acute and chronic beta3-adrenergic receptor activation. PMID: 17711991
    48. suggests a direct functional role for both HSL and adipose triglyceride lipase in hepatic lipid homeostasis PMID: 18337240
    49. These findings suggest that adipocyte triglyceride lipase and hormone-sensitive lipase differentially modulate biological processes in metabolic tissues. PMID: 18572100
    50. HSL plays a critical role in the hydrolysis of cytosolic cholesteryl esters PMID: 18664600

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  • 亚细胞定位：
    Cell membrane. Membrane, caveola. Cytoplasm, cytosol. Lipid droplet.
  • 蛋白家族：
    'GDXG' lipolytic enzyme family
  • 数据库链接：

    KEGG: mmu:16890

    STRING: 10090.ENSMUSP00000003207

    UniGene: Mm.158548