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  4. Recombinant Mouse Homeobox protein PKNOX1 (Pknox1)

Recombinant Mouse Homeobox protein PKNOX1 (Pknox1)

  • 货号：
    CSB-YP018077MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP018077MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP018077MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP018077MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP018077MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Pknox1
  • Uniprot No.：
  • 别名：
    Pknox1; Homeobox protein PKNOX1; PBX/knotted homeobox 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-436
  • 氨基酸序列
    MMATQTLSID SYQDGQQMQV VTELKTEQDP NCSDPDAEGV SPPPIESQTP MDADKQAIYR HPLFPLLALL FEKCEQSTQG SEGTTSASFD VDIENFVRKQ EKDGKPFFCE DPETDNLMVK AIQVLRIHLL ELEKVNELCK DFCSRYIACL KTKMNSETLL SGEPGSPYSP VQSQQIQSAI TGTLSPQGIV VPASALQQGN VTMATVAGGT VYQPVTVVTP QGQVVTQALS PGTIRIQNSQ LQLQLNQDLS ILHQEDGSSK NKRGVLPKHA TNVMRSWLFQ HIGHPYPTED EKKQIAAQTN LTLLQVNNWF INARRRILQP MLDSSCSETP KTKKKPAQNR PVQRFWPDSL ASGVAQATPS ELAMSEGAVV TITTPVNMNV DSLQSLSSDG ATLAVQQVMM AGQSEDESVD STEDEGGALA PTHISGLVLE NSDSLQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Activates transcription in the presence of PBX1A and HOXA1.; (Microbial infection) In complex with PBX1, binds to the 5'-TGATTGAC-3' consensus sequence in the U5 region of Moloney murine leukemia virus and promotes viral transcription.
  • 基因功能参考文献：
    1. Prep1 silencing reduces inflammatory response and increases insulin sensitivity in adipose tissue. PMID: 29474930
    2. results indicate that PKnox1 is a critical regulator of maintenance and subsequent differentiation of the c-Kit+ stage of spermatogonia in the adult testes PMID: 29293683
    3. However, Prep1 doesn't affect gluconeogenesis in the absence of insulin. Also, we observed that nuclear content of gluconeogenic transcription factor FOXO1 was greatly reduced in Prep1 knockout hepatocytes. These findings suggest that Prep1 may control hepatic insulin sensitivity by targeting FOXO1 nuclear stability. PMID: 27815072
    4. Study found that knockdown of Prep1 gene expression affects adipogenic differentiation and results in significant increase in the insulin-sensitive glucose carrier Glut4 gene expression PMID: 27193720
    5. Histone changes at the Prep1 gene may contribute to insulin resistance in individuals with type 2 diabetes PMID: 26453063
    6. Loss of the Homeodomain Transcription Factor Prep1 Perturbs Adult Hematopoiesis in the Bone Marrow. PMID: 26285139
    7. These data agree with aberrant Wnt and Fgf expression levels in the Prep1-/- ES cells with a deficient embryoid bodies (EBs) formation and differentiation. Re-establishment of the Prep1 level rescues the phenotype. PMID: 25875616
    8. In fetal liver hematopoietic stem cells (HSCs) Prep1 absence induces the interferon signaling pathway leading to premature cycling and exhaustion of fetal HSCs. PMID: 25233378
    9. Prep1 is a regulator of oxidative phosphorylation components via direct and indirect mechanisms. PMID: 24216763
    10. Loss of Pbx-Wnt-p63-Irf6 regulatory module results in clefting. PMID: 21982646
    11. In mouse liver, transcriptional activation of SHP1 gene by Prep1 attenuates insulin signal transduction and reduces glucose storage. PMID: 20864515
    12. The absence of Prep1 causes p53-dependent apoptosis of mouse pluripotent epiblast cells. PMID: 20826531
    13. excess Prep1 increases the sensitivity of cells to genotoxic stress and the extent of the apoptosis directly correlates with the level of Prep1. PMID: 20110257
    14. Prep1 has a tumor suppressor function in mice. Prep1(i/i) hypomorphic mice develop spontaneous tumors or pretumoral lesions, and transplantation of Prep1(i/i) fetal liver cells into lethally irradiated normal mice induces lymphomas. PMID: 20106730
    15. Reduced Prep1 expression caused an abnormal thymic T-cell development. PMID: 16314502
    16. Data indicate that Prep1 is an essential gene that acts upstream of and within a Pbx-Meis network that regulates multiple aspects of embryonic development. PMID: 16847320
    17. Purification of the Prep1 interactome identifies novel pathways regulated by Prep1. PMID: 17623278
    18. Data show that the homeodomain transcription factor Prep1 (pKnox1) is required for hematopoietic stem and progenitor cell activity. PMID: 17904118
    19. results obtained by genetic (Prep1 hypomorphic) and functional (Pbx1NT transgenic) inactivation of Prep1 support nonredundant roles for this homeodomain protein during different stages of T cell development PMID: 18560600
    20. Data show that Prep1 and 4EHP co-localize in the cytosol of growing mouse oocytes, and that Prep1 binds the Hoxb4 3'UTR to inhibit its translation. PMID: 19365557

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    TALE/MEIS homeobox family
  • 数据库链接：

    KEGG: mmu:18771

    STRING: 10090.ENSMUSP00000094966

    UniGene: Mm.259295