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  4. Recombinant Mouse Homeobox expressed in ES cells 1 (Hesx1)

Recombinant Mouse Homeobox expressed in ES cells 1 (Hesx1)

  • 货号：
    CSB-YP717221MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP717221MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP717221MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP717221MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP717221MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Hesx1; Hes-1; Rpx; Homeobox expressed in ES cells 1; Anterior-restricted homeobox protein; Homeobox protein ANF; Rathke pouch homeo box
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-185
  • 氨基酸序列
    MSPSLREGAQ LRESKPAPCS FSIESILGLD QKKDCTTSVR PHRPWTDTCG DSEKGGNPPL HAPDLPSETS FPCPVDHPRP EERAPKYENY FSASETRSLK RELSWYRGRR PRTAFTQNQV EVLENVFRVN CYPGIDIRED LAQKLNLEED RIQIWFQNRR AKMKRSRRES QFLMAKKPFN PDLLK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for the normal development of the forebrain, eyes and other anterior structures such as the olfactory placodes and pituitary gland. Possible transcriptional repressor. Binds to the palindromic PIII sequence, 5'-AGCTTGAGTCTAATTGAATTAACTGTAC-3'. HESX1 and PROP1 bind as heterodimers on this palindromic site, and, in vitro, HESX1 can antagonize PROP1 activation.
  • 基因功能参考文献：
    1. Hesx1 plays a significant role in sustaining pluripotency in embryonic stem cells.Hesx1 transcriptionally suppresses differentiation-related genes. PMID: 26188092
    2. Wnt inhibition during the first day has larger impact on the activation of Hesx1 and Six3, suggesting that in embryos Wnt inhibition caused by Dkk1 contributes greatly in the establishment of the anterior forebrain precursor. PMID: 24520934
    3. Variability in Hes1 expression therefore helps to explain why STAT3 responsiveness varies between individual ES cells, and this in turn helps to explain why pluripotent cells commit to differentiate asynchronously.. PMID: 23649667
    4. Studies suggest that TLE1 and TLE3 might also play roles independent of HESX1 by interacting with other transcription factors like PROP1. PMID: 20181723
    5. Data suggest that formation of a heterodimer between HESX1 and PROP1 allows HESX1 to become active, and that PROP1 then replaces HESX1 to advance to the middle stage of pituitary development. PMID: 19879326
    6. A novel inducible element, activated by contact with Rathke's pouch, is present in the regulatory region of the Hesx1 homeobox gene. PMID: 12885556
    7. the domains of Hes-1 needed to block adipogenesis coincide with those necessary for transcriptional repression. PMID: 16282371
    8. Conserved regulatory elements establish the dynamic expression of Rpx in early development. PMID: 16527264
    9. The absence of Hesx1 leads to a posterior transformation of the anterior forebrain (AFB) during mouse development. PMID: 17360769
    10. Haploinsufficiency of both Six3 and Hesx1 leads to severe pituitary dysmorphogenesis during development, which results in hypopituitarism with dramatic postnatal growth retardation. PMID: 18775421
    11. Hesx1(I26T/I26T) embryos show pituitary defects comparable with Hesx1(-/-) mouse mutants, with frequent occurrence of ocular abnormalities, although the telencephalon develops normally PMID: 19093031

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    ANF homeobox family
  • 组织特异性：
    High levels found in the embryonic liver, lower level expression seen in the viscera, amnion and yolk sac.
  • 数据库链接：

    KEGG: mmu:15209

    STRING: 10090.ENSMUSP00000041999

    UniGene: Mm.4802