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  4. Recombinant Mouse Histone-lysine N-methyltransferase SUV39H1 (Suv39h1)

Recombinant Mouse Histone-lysine N-methyltransferase SUV39H1 (Suv39h1)

  • 货号：
    CSB-YP022972MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP022972MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP022972MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP022972MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP022972MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Suv39h1
  • Uniprot No.：
  • 别名：
    Suv39h1; Suv39h; Histone-lysine N-methyltransferase SUV39H1; EC 2.1.1.355; Histone H3-K9 methyltransferase 1; H3-K9-HMTase 1; Position-effect variegation 3-9 homolog; Suppressor of variegation 3-9 homolog 1; Su(var)3-9 homolog 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-412
  • 氨基酸序列
    MAENLKGCSV CCKSSWNQLQ DLCRLAKLSC PALGVSKKNL YDFEVEYLCD YKKIREQEYY LVKWRGYPDS ENTWEPRQNL KCIRVLKQFH KDLERELVRR HRRSKPPRHL DPNLANYLVQ KAKQRRALQR WEQELNAKRS HLGRITVENE VDLDGPPRSF VYINEYRVGE GITLNQVAVG CECQDCLLAP TGGCCPGASL HKFAYNDQGQ VRLKAGQPIY ECNSRCCCGY DCPNRVVQKG IRYDLCIFRT NDGRGWGVRT LEKIRKNSFV MEYVGEIITS EEAERRGQIY DRQGATYLFD LDYVEDVYTV DAAYYGNISH FVNHSCDPNL QVYNVFIDNL DERLPRIAFF ATRTIWAGEE LTFDYNMQVD PVDMESTRMD SNFGLAGLPG SPKKRVRIEC KCGTTACRKY LF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as repression of MYOD1-stimulated differentiation, regulation of the control switch for exiting the cell cycle and entering differentiation, repression by the PML-RARA fusion protein, BMP-induced repression, repression of switch recombination to IgA and regulation of telomere length. Component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. Recruited by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1, contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation.
  • 基因功能参考文献：
    1. SUV39H augments intracellular reactive oxygen species levels in a SIRT1-dependent manner. PMID: 28361889
    2. this novel defense mechanism against mycobacteria represents a surrogate function of the epigenetic modulator, SUV39H1, and operates by interfering with their cell-cell adhesion ability. PMID: 29170282
    3. the Suv39h1 paralog, is identified as a unique enhancer of HP1alpha sumoylation PMID: 27426629
    4. The s show here that the mouse Suv39h2 enzyme differs from Suv39h1 by containing an N-terminal basic domain that facilitates retention at mitotic chromatin and provides an additional affinity for major satellite repeat RNA. PMID: 28760199
    5. Suv39h1 plays a critical role in marking chromatin to silence stem/memory genes during CD8+ T effector terminal differentiation PMID: 29326266
    6. Histone methyltransferase Suv39h1 attenuates high glucose-induced fibronectin and p21(WAF1) in mesangial cells PMID: 27373678
    7. Overexpression of miR-125b and inhibition of its target, SUV39H1, in aging individuals reduces hematopoietic stem cells capacity of B cells generation. PMID: 27304919
    8. Activation of p38alpha displaces KMT1A from MyoD to initiate myogenic gene expression upon induction of myoblasts differentiation. PMID: 27551368
    9. Oct4P4 lncRNA forms a complex with the SUV39H1 HMTase to direct the imposition of H3K9me3 and HP1alpha to the promoter of the ancestral Oct4 gene, located on chromosome 17, leading to gene silencing and reduced mESC self-renewal. PMID: 26158551
    10. Provide genetic evidence that Suv39h1 controls telomere homeostasis and mediates resistance to oncogenic stress in vivo, conferring resistance to skin carcinogenesis. PMID: 25789788
    11. Repeat-associated, Suv39h-dependent H3K9me3 displays a dynamic distribution that differs in the epigenome of pluripotent versus more committed cells. PMID: 24981170
    12. These findings indicate that Suv39h1 enhances AP-2alpha-mediated transcriptional repression of C/EBPalpha in an epigenetic manner and further inhibits adipocyte differentiation. PMID: 24732798
    13. A-type lamins interact with SUV39H1, and prelamin A/progerin exhibits enhanced binding capacity to SUV39H1, protecting it from proteasomal degradation and, consequently, increasing H3K9me3 levels. PMID: 23695662
    14. Data indicate that loss of suv39h1 prevents Myc-induced chromosomal instability. PMID: 23340989
    15. Smads recruit H3K9 methyltransferases Suv39h1 to the IL-2 promoter, thereby inducing suppressive histone methylation and inhibiting T cell receptor-mediated IL-2 transcription. PMID: 21862595
    16. SirT1 preserves heterochromatin structure: it modulates Suv39h1 protein levels in stress conditions by preventing MDM2-mediated polyubiquitination at K87. PMID: 21504832
    17. novel role for microRNA in the epigenetic regulation of inflammatory genes in vascular smooth muscle cells of diabetic mice through downregulation of SUV39h1. PMID: 20699419
    18. Ring1B and Suv39h1 have mutually exclusive roles in regulating distinct chromatin states at key developmental genes, and play a part in how lineage specification can be reinforced during early development PMID: 20573702
    19. SUV39h1 associated with HP1 beta in fibrillarin-positive nucleolar regions. PMID: 20033197
    20. SUV39h and A-type lamins likely play a key role in telomere maintenance and telomere nuclear architecture. PMID: 20069564
    21. Forms complex with AML1. PMID: 12917624
    22. Taken together, the results indicate epigenetic regulation of telomere length in mammals by Suv39h1 and Suv39h2. PMID: 14702045
    23. Suv39h1 interacts with Smads and cooperates in BMP-induced repression. PMID: 15107829
    24. Suv39h1 activity inhibits the activity of a sequence-specific DNA-binding protein that represses switch recombination to IgA. PMID: 16818776
    25. Silences gene transcription on the promoter of muscle genes, providing a necessary checkpoint between proliferation and differentiation. PMID: 16858404
    26. findings demonstrate a functional link between the heterochromatin-related histone methyltransferase SUV39H1 and the histone deacetylase SIRT1 PMID: 18004385
    27. the Suv39h-HP1 pathway is not essential for enrichment and mitotic protection of cohesin at centromeres in mammalian cells PMID: 18075750
    28. Inactivation leads to the loss of histone 4 trimethylated at lysine 20 and increased levels of its substrate, histone 4 monomethylated at lysine 20. PMID: 18724273
    29. role in co-regulating growth control and differentiation during mammalian development PMID: 11520670

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  • 亚细胞定位：
    Nucleus. Nucleus lamina. Nucleus, nucleoplasm. Chromosome, centromere. Note=Associates with centromeric constitutive heterochromatin.
  • 蛋白家族：
    Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, Suvar3-9 subfamily
  • 组织特异性：
    Widely expressed.
  • 数据库链接：

    KEGG: mmu:20937

    STRING: 10090.ENSMUSP00000111301

    UniGene: Mm.479743