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  4. Recombinant Mouse High affinity choline transporter 1 (Slc5a7), partial

Recombinant Mouse High affinity choline transporter 1 (Slc5a7), partial

  • 中文名称：
    小鼠Slc5a7重组蛋白
  • 货号：
    CSB-YP806365MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Slc5a7重组蛋白
  • 货号：
    CSB-EP806365MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Slc5a7重组蛋白
  • 货号：
    CSB-EP806365MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Slc5a7重组蛋白
  • 货号：
    CSB-BP806365MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Slc5a7重组蛋白
  • 货号：
    CSB-MP806365MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Slc5a7
  • Uniprot No.：
  • 别名：
    Slc5a7; Cht1; High affinity choline transporter 1; Hemicholinium-3-sensitive choline transporter; CHT; Solute carrier family 5 member 7
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transmembrane transporter that imports choline from the extracellular space to the neuron with high affinity. Choline uptake is the rate-limiting step in acetylcholine synthesis. Sodium ion- and chloride ion-dependent.
  • 基因功能参考文献：
    1. Our findings suggest that CHT protein levels preferentially impact the capacity for ACh release afforded by mobilization of reserve pool vesicles PMID: 27013347
    2. These data provide evidence for an epithelium-independent non-vesicular, non-quantal ACh release in the mouse trachea involving low-affinity choline transporters. PMID: 26278668
    3. bacterial artificial chromosome CHT mice thus represent a novel tool to examine both the positive and negative impact of constitutively elevated cholinergic signaling capacity PMID: 24274995
    4. These findings reveal compensatory mechanisms that, in the context of moderate cognitive challenges, can overcome the performance deficits expected from the significantly reduced ACh capacity of CHT+/- cholinergic terminals. PMID: 23958450
    5. CHT association with cholesterol-rich rafts is critical for its function PMID: 24127780
    6. This study showed that a constitutive loss of CHT expression in hemizygous mice leads to a significant reduction in basal extracellular dopamine levels in the nucleus accumbens. PMID: 23939187
    7. This study demonistrated that CHT support for neuromuscular signaling involves contributions to ACh synthesis as well as cholinergic synaptic vesicle availability. PMID: 20888396
    8. CHT1 knockout mice have tachycardia and hypertension at rest. PMID: 20601463
    9. CHT1 binds to Par-4 and inhibits choline uptake when its incorporation is reduced on the cell surface PMID: 15090548
    10. CHT is an essential and regulated presynaptic component of cholinergic signaling PMID: 15173594
    11. CHT1 was expressed early in CNS development, predominantly in the regions containing cholinergic neurons. NGF AND BMP4 increased CHT mRNA accumulation in primary septal cultures. PMID: 15885806
    12. Choline transporter hemizygosity results in adequate baseline acetylcholine stores, sufficient to sustain many phenotypes, but normal sensitivities to physical and/or pharmacological challenge require full cholinergic signaling capacity. PMID: 17010154
    13. Interestingly, although CHT is expressed predominantly in cholinergic cells, its regulation appears to be distinct from that of the cholinergic locus. PMID: 17320829
    14. Real-time RT-PCR revealed significant decreases in CHT1 mRNA in the lung of sensitized and allergen challenged animals. PMID: 17328924
    15. adaptor proteins have important functions for CHT trafficking in neuronal cells PMID: 18554297
    16. Synapses persist for at least 2-3 months without postsynaptic activity; however, the presynaptic terminals lack high-affinity choline transporters (CHTs), and as a result, they are quickly depleted of transmitter. PMID: 19186169

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  • 亚细胞定位：
    Membrane; Multi-pass membrane protein. Cell membrane. Cell junction, synapse.
  • 蛋白家族：
    Sodium:solute symporter (SSF) (TC 2.A.21) family
  • 组织特异性：
    Found in spinal cord, brain-stem, mid-brain and striatum. Specific for cholinergic neurons.
  • 数据库链接：

    KEGG: mmu:63993

    STRING: 10090.ENSMUSP00000093379

    UniGene: Mm.155241