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  4. Recombinant Mouse Hepatocyte nuclear factor 3-beta (Foxa2)

Recombinant Mouse Hepatocyte nuclear factor 3-beta (Foxa2)

  • 货号：
    CSB-YP008795MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP008795MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP008795MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP008795MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP008795MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Foxa2; Hnf3b; Tcf-3b; Tcf3bHepatocyte nuclear factor 3-beta; HNF-3-beta; HNF-3B; Forkhead box protein A2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-459
  • 氨基酸序列
    MLGAVKMEGH EPSDWSSYYA EPEGYSSVSN MNAGLGMNGM NTYMSMSAAA MGGGSGNMSA GSMNMSSYVG AGMSPSLAGM SPGAGAMAGM SGSAGAAGVA GMGPHLSPSL SPLGGQAAGA MGGLAPYANM NSMSPMYGQA GLSRARDPKT YRRSYTHAKP PYSYISLITM AIQQSPNKML TLSEIYQWIM DLFPFYRQNQ QRWQNSIRHS LSFNDCFLKV PRSPDKPGKG SFWTLHPDSG NMFENGCYLR RQKRFKCEKQ LALKEAAGAA SSGGKKTAPG SQASQAQLGE AAGSASETPA GTESPHSSAS PCQEHKRGGL SELKGAPASA LSPPEPAPSP GQQQQAAAHL LGPPHHPGLP PEAHLKPEHH YAFNHPFSIN NLMSSEQQHH HSHHHHQPHK MDLKAYEQVM HYPGGYGSPM PGSLAMGPVT NKAGLDASPL AADTSYYQGV YSRPIMNSS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3'. In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; Foxa1 and Foxa2 seem to have at least in part redundant roles. FOXA1 and FOXA2 are essential for hepatic specification. FOXA1 and FOXA2 are required for morphogenesis and cell differentiation during formation of the lung. FOXA1 and FOXA2 are involved in bile duct formation; they positively regulate the binding glucocorticoid receptor/NR3C1 to the IL6 promoter. FOXA1 and FOXA2 regulate multiple phases of midbrain dopaminergic neuron development; they regulate expression of NEUROG2 at the beginning of mDA neurogenesis and of NR4A2 and EN1 in immature mDA neurons. Modulates the transcriptional activity of nuclear hormone receptors; inhibits AR-mediated transcription from the LCN5 promoter. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. In pancreatic beta cells activates transcription of potassium channel subunits KCNJ11 and ABCC8. Involved in regulation of fat metabolism; activates transcriptional programs of lipid metabolism and ketogenesis at low insulin state. Involved in transcriptional regulation of MUC2 in the intestine.
  • 基因功能参考文献：
    1. Maturation of pancreatic beta-cells requires the cooperative function of Foxa2 and Pdx1. PMID: 28580283
    2. Data indicate a role for forkhead box A2 (FOXA2) in regulation of adult uterine function and fertility and mice Lacking FOXA2 in the adult uterus are infertile. PMID: 28049832
    3. The c.505T>C, p.S169P genetic variant occurs at the conserved forkhead DNA binding domain of the FOXA2 and causes a rare and unique clinical phenotype of hypopituitarism, CHI, dysmorphic features, liver, pancreas, heart and gastrointestinal abnormalities. PMID: 28973288
    4. Forkhead box A2regulates biliary heterogeneity during cholestatic liver injury in mouse models. PMID: 27639079
    5. Foxa2, a novel protein partner of the tumour suppressor menin, is deregulated in mouse and human MEN1 glucagonomas. PMID: 28188614
    6. our study demonstrates that Foxa2 is required in both alpha and beta cells for maintaining appropriate glucagon and insulin levels and for keeping glucose homeostasis. PMID: 26882312
    7. The s show herein that Nurr1 and Foxa2 interact to protect midbrain dopaminergic neurons against various toxic insults, but their expression is lost during aging and degenerative processes. PMID: 25759364
    8. s identified thousands of enhancers that are bound by the master regulators HNF4A and FOXA2 in a differentiation-dependent manner, subject to chromatin remodeling, and associated with differentially expressed target genes. PMID: 25263553
    9. this study demonstrates that SIRT1 controls the acetylation level of FOXA2 in a nutrient-dependent manner and in times of nutrient shortage the interaction between SIRT1 and FOXA2 is reduced. PMID: 24875183
    10. Hh signaling upregulated FOXA2, which induced expression of MUC2, an intestinal mucin found in Barrett's esophagus, and the MUC2-processing protein AGR2 PMID: 25083987
    11. studies have identified a novel TXNIP/miR-124a/FoxA2/IAPP signaling cascade linking the critical beta-cell signaling pathways of TXNIP and IAPP PMID: 24627476
    12. The interplay of Nurr1 and Foxa2 is the crucial determinant for dopamine phenotype acquisition during midbrain dopaminergic neuron development. PMID: 24496614
    13. Nuclear HNF3beta signaling is regulated by estrogen receptors in non-tumor intestinal mucosa. PMID: 24104551
    14. Nato3 induces ectopic Foxa2-positive cells and indirectly downregulates Nkx2.2 expression. PMID: 24401371
    15. Foxa2 is required for normal airway epithelial differentiation, and its deletion causes goblet-cell metaplasia and Th2-mediated pulmonary inflammation during postnatal development. PMID: 23822876
    16. Findings suggest that Onecut1 suppresses MafA gene expression through the Foxa2-binding site. PMID: 23775071
    17. Data indicate that plasma lipids, lipid absorption, and microsomal triglyceride transfer protein (MTP), FoxO1, and FoxA2 levels are lower at night and at mealtime in apoAIV-/- mice. PMID: 23729668
    18. Glucagon-induced acetylation of Foxa2 regulates hepatic lipid metabolism. PMID: 23416070
    19. Mst1/2 kinases are critical for orchestration of transcription factors involved in surfactant protein homeostasis and prevention of respiratory distress syndrome, and phosphorylate Foxa2, regulating pneumocyte maturation and surfactant protein expression PMID: 23620511
    20. These studies suggest that prostate cancer TRAMP mice neuroendocrine tumors can form in the absence of Foxa2 by an up regulation of Mash1. PMID: 23060003
    21. The variant histone H2A.Z and the winged helix transcription factor Foxa2 both act to regulate nucleosome depletion and gene activation, thus promoting ES cell differentiation, whereas DNA methylation promotes nucleosome occupation and suppresses gene expression. PMID: 23260146
    22. Foxa2 regulates directly and positively key determinants of mDA neurons, including Lmx1a, Lmx1b, Msx1 and Ferd3l, while negatively inhibiting transcription factors expressed in ventrolateral midbrain. PMID: 22696295
    23. Pyocyanin induces goblet cell hyperplasia and mucus hypersecretion by inactivating FoxA2. PMID: 22103442
    24. FoxA family members are crucial regulators of the hypertrophic chondrocyte differentiation program. PMID: 22595668
    25. The promoter regions of Sox17 and Foxa2 are subjected to histone acetylation regulation. PMID: 22132182
    26. Study uncovered a central role for Foxa1 and Foxa2 in controlling estrogen and androgen signaling through recruitment of ERalpha and AR to their relevant targets in the liver, thereby explaining the sexual dimorphism of liver cancer in mammals PMID: 22265403
    27. Whereas only approximately 10% of binding sites are conserved, gene-centric analysis reveals that about 50% of genes with nearby TF occupancy are shared across species for both hepatic FOXA2 and adipocyte PPARgamma PMID: 21292830
    28. Data reveal a highly regulated temporal-spatial pattern of alpha5 nAChR subunit expression during important periods of lung morphogenesis by FoxA2. PMID: 21682884
    29. Data suggest that Nato3 is a direct target of Foxa2, a transcription activator and effector of Sonic hedgehog, the hallmark regulator of neural tube floor plate induction and spinal cord development. PMID: 20849957
    30. Foxa2 is required for uterine gland development and pregnancy in mice. PMID: 20484741
    31. We globally identified binding sites for FOXA2 in adult mouse islets and liver, PDX1 in islets, and HNF4A in liver. Novel insights into how FOXA2, PDX1, and HNF4A cooperate to drive islet- and liver-specific gene expression were generated. PMID: 20551221
    32. The function of the intronic Gata4 enhancer is dependent upon a conserved Forkhead transcription factor-binding site, which is bound by recombinant FoxA2 in vitro. PMID: 20692247
    33. The respiratory epithelium plays a central role in the regulation of Th2-mediated inflammation and innate immunity in the developing lung in a process regulated by Foxa2. PMID: 20483781
    34. obtained evidence that Lmx1a and Lmx1b cooperate with Foxa2 to specify mesDA neuron identity by gain-of-function approaches using transgenic mice PMID: 20035737
    35. Foxa2 plays an integral role in the formation of axial mesendoderm, which is required to maintain the specification of the forebrain and the anterior definitive endoderm PMID: 11846474
    36. Profound defects in pancreatic beta-cell function in mice with combined heterozygous mutations in Pdx-1, Hnf-1alpha, and Hnf-3beta PMID: 11904435
    37. controls Pdx1 gene expression in pancreatic beta-cells in vivo PMID: 12145169
    38. This protein up-regulates vitronectin expression during tretinoin-induced differentiation in mouse neuroblastoma cells. PMID: 12441652
    39. In reduced hepatocyte glycogen storage, Foxa2 transgenic mice survive by maintaining serum levels of glucose through gluconeogenesis using deaminated amino acids with dicarboxylate products of peroxisomal lipid beta-oxidation through Krebs cycle. PMID: 12774021
    40. Foxa2 regulates multiple pathways of insulin secretion. PMID: 15314688
    41. Foxa2 regulates a complex pulmonary program of epithelial cell maturation required for transition to air breathing at birth. PMID: 15452354
    42. Foxa2 plays unique as well as overlapping with Foxa1 rolres during morphogenesis and in the function of adult organs. PMID: 15567715
    43. in normal mice, plasma insulin inhibits the forkhead transcription factor Foxa2 by nuclear exclusion, and in the fasted (low insulin) state, Foxa2 activates transcriptional programmes of lipid metabolism and ketogenesis PMID: 15616563
    44. Foxa1 deficient mice are severely hypoglycemic and die within the first week of life. PMID: 15680365
    45. suggest that Foxa proteins are important in prostate carcinogenesis; in particular, Foxa2 may be involved in progression of prostate cancer to androgen independence PMID: 16001449
    46. HNF6 binds to and regulates its target promoters in vivo in the presence and absence of Foxa2 PMID: 16055718
    47. Results suggest that Foxa2 is required for execution of the hepatic gluconeogenic program by integrating the transcriptional response of the hepatocyte to hormonal stimulation. PMID: 16098831
    48. as binding proteins for the 3' motif, tead activates the Foxa2 enhancer core element in the mouse node in cooperation with a second factor that binds to the 5' element PMID: 16207754
    49. Foxa2/Pgc-1beta regulate hepatic lipid homeostasis by affecting the clearance rate of fatty acids through oxidation and/or secretion of lipids in response to insulin. PMID: 16459311
    50. mouse protein targeting to glycogen (PTG) promoter is regulated by the FoxA2 forkhead protein and by 3',5'-cyclic adenosine 5'-monophosphate in H4IIE hepatoma cells PMID: 16627590

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Note=Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner; in response to insulin signaling via AKT1 is exported from the nucleus.
  • 组织特异性：
    Restricted mainly to endoderm-derived tissues (lung, liver, stomach, and small intestine). Expressed in epididymis with region-specific expression pattern: no expression is observed in initial segment, low expression in proximal caput, gradiently higher l
  • 数据库链接：

    KEGG: mmu:15376

    STRING: 10090.ENSMUSP00000045918

    UniGene: Mm.938