Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Mouse Guanine nucleotide-binding protein subunit alpha-12 (Gna12)

Recombinant Mouse Guanine nucleotide-binding protein subunit alpha-12 (Gna12)

  • 货号：
    CSB-YP009584MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP009584MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP009584MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP009584MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Gna12; Gna-12; Guanine nucleotide-binding protein subunit alpha-12; G alpha-12; G-protein subunit alpha-12
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-379
  • 氨基酸序列
    MSGVVRTLSR CLLPAEAGAR ERRAGAARDA EREARRRSRD IDALLARERR AVRRLVKILL LGAGESGKST FLKQMRIIHG REFDQKALLE FRDTIFDNIL KGSRVLVDAR DKLGIPWQHS ENEKHGMFLM AFENKAGLPV EPATFQLYVP ALSALWRDSG IREAFSRRSE FQLGESVKYF LDNLDRIGQL NYFPSKQDIL LARKATKGIV EHDFVIKKIP FKMVDVGGQR SQRQKWFQCF DGITSILFMV SSSEYDQVLM EDRRTNRLVE SMNIFETIVN NKLFFNVSII LFLNKMDLLV EKVKSVSIKK HFPDFKGDPH RLEDVQRYLV QCFDRKRRNR SKPLFHHFTT AIDTENIRFV FHAVKDTILQ ENLKDIMLQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF12/LARG). GNA12-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1). GNA12-dependent Rho signaling also regulates protein phosphatese 2A activation causing dephosphorylation of its target proteins. Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway and up-regulating proinflammatory cytokine production. Inhibits CDH1-mediated cell adhesion in process independent from Rho activation. Together with NAPA promotes CDH5 localization to plasma membrane. May play a role in the control of cell migration through the TOR signaling cascade.
  • 基因功能参考文献：
    1. Galpha12 is required for the development of kidney cysts induced by Pkd1 mutation in mouse autosomal dominant polycystic kidney disease. PMID: 27505895
    2. Our data reveal a previously unknown role for Galpha12 in regulating efferocytosis and that renal tubular epithelial cells require KIM-1 to mediate this process. PMID: 26697979
    3. Selective inhibition of G12/13 signaling in macrophages can augment atheroprotective B-cell populations and ameliorate atherosclerosis. PMID: 26603156
    4. Gpr56 regulates oligodendrocyte development via interactions with Galpha12/13 and RhoA PMID: 25607772
    5. results suggest that KIM-1 is an endogenous protective mechanism against renal ischemia-reperfusion injury through inhibition of Galpha12. PMID: 25759266
    6. Galpha12 may play an important role in promoting membrane trafficking and exocytosis for basal and thrombin-induced vWF secretion, in a process involving SNAP, Galpha12/13 and Galphaq/11 PMID: 24081657
    7. Data indicate that Pyk2 is a common signaling effector downstream of both G12/13 and integrin alphaIIbbeta3 signaling, which contributes to thromboxane generation. PMID: 23640884
    8. PRR5L degradation promotes mTORC2-mediated PKC-delta phosphorylation and cell migration downstream of Galpha12 in fibroblasts and pulmonary fibrosis. PMID: 22609986
    9. Expression analysis reveals that production of 5-HT7 and Galpha12 proteins in the hippocampus undergoes strong regulation with a pronounced transient increase during early postnatal stages. PMID: 22378867
    10. that Galpha(i2)-induced signaling can act as a counterbalance to MuRF1-mediated atrophy, indicating that receptors that act through Galpha(i2) might represent potential targets for preventing skeletal muscle wasting. PMID: 22126963
    11. Galpha(12/13) regulate AP-1-dependent CYR61 induction in vascular smooth muscle, promoting migration, and they are upregulated with CYR61 in arteriosclerotic lesions. PMID: 21212405
    12. These results suggest that Galpha12 may be a critical signaling molecule in TCR-induced IL-2 production and also relay a signal to suppress Th2 and Th17 cell differentiation. PMID: 20233578
    13. The G alpha(12)-mediated signaling pathway functionally interacts not only with the G alpha(13)- but also with the G alpha(q/11)-mediated signaling systems. PMID: 12077299
    14. Hsp90 binding and acylation of Galpha12 results in localization to lipid rafts PMID: 12117999
    15. intracellular Sphingosine 1-phosphate signaled "inside out" through its cell-surface receptors linked to G12/13-mediated stress fiber formation, important for cell motility PMID: 12963721
    16. Galpha(12) stimulates cell proliferation and neoplastic transformation of NIH3T3 cells by attenuating p38MAPK-associated apoptotic responses, while activating the mitogenic responses through the stimulation of ERK- and JNK-mediated signaling pathways. PMID: 14712227
    17. results from differential proteome analysis report a role for SET in Galpha(12)-mediated signaling pathways and a role for Galpha(12) in the regulation of the leukemia-associated SET-protein expression PMID: 15595726
    18. Data demonstrate distinct roles for 5-HT7 receptor/G12 signaling pathways in neurite outgrowth and retraction PMID: 16120784
    19. identified Galpha12/13-proteins as essential components linking extracellular signals to localized Rho-Dia1 function during directed cell movement PMID: 16251183
    20. Galpha12 stimulates the phosphatidylinositol 3-kinase (PI3K)-AKT signaling pathway through PDGFRalpha. PMID: 16354679
    21. G12/G13 family G proteins contribute to the formation of the mature marginal zone B (MZB) cell compartment both by controlling MZB cell migration and by regulating MZB cell precursor maturation PMID: 16920934
    22. Galpha(12) specifically regulates NF-kappaB-mediated COX-2 induction by S1P downstream of S1P(1), S1P(3), and S1P(5), in a process mediated by the JNK-dependent ubiquitination and degradation of IkappaBalpha PMID: 17098744
    23. G alpha(12/13) regulate basal p53 levels via mdm4, which constitutes a cell signaling pathway distinct from p53 phosphorylations elicited by genotoxic stress. PMID: 17510313
    24. Data suggest that Galpha12 and Galpha13 transmit a JNK-dependent signal for Nrf2 ubiquitination, whereas Galpha13 regulates Rho-PKC delta-mediated Nrf2 phosphorylation, which is negatively balanced by Galpha12. PMID: 17591699
    25. PDGFRalpha and JAK3 have roles in Galpha(12)-mediated cell proliferation PMID: 18038259
    26. G12-G13-LARG-mediated signaling in vascular smooth muscle is required for salt-induced hypertension PMID: 18084302
    27. AC7 is a specific downstream effector of the G(12/13) pathway PMID: 18541530
    28. Galpha(12) and Galpha(13) regulate expression of TGFbeta1 gene through an increase in Rho/Rac-dependent AP-1 activity, implying that the G-protein-coupled receptor (GPCR)-Galpha(12) pathway is involved in TGFbeta1-mediated transdifferentiation process PMID: 19151758
    29. Constitutive Gs-mediated, but not G12-mediated, activity of the 5-hydroxytryptamine 5-HT7(a) receptor is modulated by the palmitoylation of its C-terminal domain. PMID: 19715731

    显示更多

    收起更多

  • 亚细胞定位：
    Cell membrane; Lipid-anchor. Lateral cell membrane; Lipid-anchor. Cytoplasm.
  • 蛋白家族：
    G-alpha family, G(12) subfamily
  • 数据库链接：

    KEGG: mmu:14673

    STRING: 10090.ENSMUSP00000000153

    UniGene: Mm.370185