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  4. Recombinant Mouse Growth factor receptor-bound protein 2 (Grb2)

Recombinant Mouse Growth factor receptor-bound protein 2 (Grb2)

  • 货号：
    CSB-YP009889MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP009889MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP009889MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP009889MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP009889MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Grb2; Growth factor receptor-bound protein 2; Adapter protein GRB2; SH2/SH3 adapter GRB2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-217
  • 氨基酸序列
    MEAIAKYDFK ATADDELSFK RGDILKVLNE ECDQNWYKAE LNGKDGFIPK NYIEMKPHPW FFGKIPRAKA EEMLSKQRHD GAFLIRESES APGDFSLSVK FGNDVQHFKV LRDGAGKYFL WVVKFNSLNE LVDYHRSTSV SRNQQIFLRD IEQMPQQPTY VQALFDFDPQ EDGELGFRRG DFIHVMDNSD PNWWKGACHG QTGMFPRNYV TPVNRNV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.; Does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
  • 基因功能参考文献：
    1. Study shows that growth factor receptor binding protein 2 carboxyl-terminal SH3 domain can bivalently associate with two ligands, in an SH3 dependent manner. Extrapolating the results of this study to the in vivo conditions, Grb2 should bind the SLP65 transducer module first, and then Vav should associate. PMID: 28455498
    2. Our findings position Grb2 as a key adaptor that integrates various cytokines response in cycling Hematopoietic Stem and Progenitor Cell . PMID: 28964849
    3. Themis1 acts as a positive regulator of TCR signaling during thymocyte development by promoting Vav1 activity and Grb2 stability PMID: 27188442
    4. Myogenic differentiation depends on the expression regulation patterns of Grb2 and N-WASP. PMID: 27965114
    5. Grb2-deficient T cells show defects in T cell development, increased Th1 and Th17 cell differentiation capacities, and impaired proliferation after activation by dendritic cells, which likely reduce the clinical symptoms of EAE. PMID: 26921310
    6. GRB2 physically links cyt-PTPe with Src and enables cyt-PTPe to activate Src downstream of activated integrins in osteoclast-like cells. PMID: 25381250
    7. SUMOylation of Grb2 enhances the ERK activity by increasing its binding with Sos1. PMID: 24775912
    8. Data indicate that growth factor receptor protein binding protein 2 (Grb2) is upregulated and regulated by Forkhead Box D3 (Foxd3), and pregulated Grb2 interacts with huntingtin (Htt). PMID: 24116161
    9. Grb2 contributes to immunoreceptor tyrosine-based activation motif signaling in platelets during hemostasis and thrombosis. PMID: 24265393
    10. Grb2 is not required for the establishment of the glomerular filtration barrier in vivo. PMID: 23226445
    11. Study shows that the ability of Sos1/Grb2 to appropriately regulate pluripotency and differentiation factors and to initiate primitive endoderm development requires collective binding of multiple Sos1/Grb2 domains to their protein and phospholipid ligands. PMID: 23452850
    12. study reports that a heterotrimeric protein complex exists between PLD2, Grb2, and WASp in vivo, which is integral to phagocytosis PMID: 21930784
    13. Ablation of Grb2 in B cells results in enhanced B-cell receptor signaling; however, mutant B cells do not form germinal centers in the spleen after antigen stimulation. PMID: 21508326
    14. Themis2 was inducibly tyrosine phosphorylated upon LPS challenge and interacted with adaptor protein Grb2 (Q60631). PMID: 20644716
    15. show that conditional ablation of Grb2 in thymocytes severely impairs both thymic positive and negative selections. PMID: 20498059
    16. The surface of Vav N-terminal SH3 which binds to Grb2 C-terminal SH3 was elucidated by chemical shift mapping experiments using NMR. PMID: 11885979
    17. SH3 domains of STAM2 and Grb2 retain the moderate characteristics of recognizing their ligand proteins like other SH3 domains for signal protein binding PMID: 13129930
    18. growth factor receptor-bound protein 2 (Grb2), that specifically binds AbetaPP, phosphorylated in Tyr(682). PMID: 15054097
    19. ligand binding reduces the overall protein flexibility, possibly via slightly different mechanisms for the single SH2 domain and the full-length Grb2 protein PMID: 15210125
    20. Results suggest that MUC20 is a novel regulator of the Met signaling cascade which has a role in suppression of the Grb2-Ras pathway. PMID: 15314156
    21. FcgammaRIIB docking site for the SH2 domain-containing adapters Grb2 and Grap PMID: 15456754
    22. Grb2 heterozygosity does not effect tumorigenesis in pten-deficient mice PMID: 15492213
    23. These results suggest that merlin contributes, via its protein-to-protein interaction with Grb2 and consequent inhibition of the MAPK pathways. PMID: 16405865
    24. These findings indicate that Dok-3 sequesters Grb2 from Shc and inhibits the Ras-Erk pathway downstream of PTKs. PMID: 16436051
    25. Inner cell mass (ICM) develops as a mosaic of epiblast (EPI) and primitive endoderm (PE) progenitors at E3.5, dependent on Grb2-Ras-MAP kinase signaling, followed by later segregation of the progenitors into the appropriate cell layers. PMID: 16678776
    26. The redox-dependent enhancement of Grb2 recruitment to LMW-PTP ultimately leads to an improvement of ERK activation, likely triggering a prosurvival signal against the oxidant environment. PMID: 16890200
    27. Grb2/Mitogen-Activated Protein Kinase Kinases pathway primarily mediates Nanog gene repression upon embryonic stem cell differentiation into primitive endoderm PMID: 16908534
    28. Subcellular localization of Grb2 by the adaptor protein Dok-3 restricts the intensity of Ca2+ signaling in B cells. PMID: 17290227
    29. suggests a role for Grb2 as an intermediary linking Cbl ubiquitin ligase activity to this process PMID: 17449471
    30. Grb2 Src homology-2 domain-mediated interactions have a critical role in metastatic spread of primary solid tumors and Grb2 is inhibited by C90 PMID: 17616655
    31. Grb2 binding to Tyr284 in TbetaR-II is essential for mammary tumor growth and metastasis stimulated by TGF-beta PMID: 18174260
    32. Cells with active Gas6/Axl signal through Grb2 and the PI3 kinase/Akt pathways. PMID: 18346204
    33. Grb2 may have a role in tumor growth, invasiveness and metastasis PMID: 18425376
    34. Grb2 coupling with its Met receptor influences olfactory interneuron precursor migration along the rostral migratory stream. PMID: 18524894
    35. Recruitment of the cytoplasmic adaptor Grb2 to surface IgG and IgE provides antigen receptor-intrinsic costimulation to class-switched B cells. PMID: 19668218

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Endosome. Golgi apparatus.
  • 蛋白家族：
    GRB2/sem-5/DRK family
  • 组织特异性：
    Expressed in macrophages.
  • 数据库链接：

    KEGG: mmu:14784

    STRING: 10090.ENSMUSP00000021090

    UniGene: Mm.439649