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  4. Recombinant Mouse Glial cell line-derived neurotrophic factor (Gdnf)

Recombinant Mouse Glial cell line-derived neurotrophic factor (Gdnf)

  • 货号：
    CSB-YP009356MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP009356MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP009356MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP009356MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP009356MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    GdnfGlial cell line-derived neurotrophic factor; mGDNF; Astrocyte-derived trophic factor; ATF
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    78-211
  • 氨基酸序列
    SPD KQAAALPRRE RNRQAAAASP ENSRGKGRRG QRGKNRGCVL TAIHLNVTDL GLGYETKEEL IFRYCSGSCE SAETMYDKIL KNLSRSRRLT SDKVGQACCR PVAFDDDLSF LDDNLVYHIL RKHSAKRCGC I
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
  • 基因功能参考文献：
    1. lack of Kir6.2 promoted neuronal differentiation via inhibiting the downregulation of glia cell line-derived neurotrophic factor (GDNF), which negatively related to the level of microRNA-133b. PMID: 29564810
    2. These results imply an important role of GDNF in restoring restrictive blood-nerve barrier function in vivo PMID: 29913111
    3. These results suggest that chronic overexpression of GDNF in brain astrocytes exerts an opposing influence on nigrostriatal dopamine metabolism and neurotransmission. PMID: 28645787
    4. GDNF promotes self-renewal by blocking differentiation and not by promoting proliferation. PMID: 29440301
    5. Cross-platform single cell analysis of kidney development shows stromal cells express Gdnf. PMID: 29183737
    6. Our study provides, for the first time, a global analysis of phosphorylation events in spermatogonial progenitor cells (SPCs) in response to GDNF, and we have identified activation of mTORC1 signaling through ERK kinase-mediated phosphorylation of multiple sites of raptor protein as an important pathway for SPC proliferation. PMID: 28408662
    7. Data show that NEDL2 regulates GDNF/Ret/Akt pathway depends on its Nedd8 ligase activity rather than ubiquitin ligase activity. PMID: 27119228
    8. Six2 mediates the protective effects of GDNF on damaged DA neurons by regulating Smurf1 expression. PMID: 27148690
    9. Ret is essential to mediate GDNF's neuroprotective and neuroregenerative effect in a Parkinson disease mouse model. PMID: 27607574
    10. identified IFNg, Neurturin (Nrtn), and glial-derived neurotrophic factor (GDNF) as ligands with unexpected roles in promoting neurogenic differentiation of Neural Precursor Cells in vivo. PMID: 27545711
    11. Using an organ culture system for prostate development and Ret mutant mice, we demonstrate that RET-mediated GDNF signaling in UGS increases proliferation of mesenchyme cells and suppresses androgen-induced proliferation and differentiation of prostate epithelial cells, inhibiting prostate development. PMID: 28506996
    12. The GDNF-GFRalpha1 complex is essential for proper hippocampal circuit development. PMID: 27707798
    13. In SOD1(G93A) spinal cords, we verified a strict correlation in the expression of the TNFalpha, TNFR1 and GDNF triad at different stages of disease progression. Yet, ablation of TNFR1 completely abolished GDNF rises in both SOD1(G93A) astrocytes and spinal cords, a condition that accelerated motor neuron degeneration and disease progression PMID: 27288458
    14. GDNF signals were able to induce the stratified aggregate formation of GFRalpha1-positive undifferentiated spermatogonia PMID: 27255992
    15. The Gdnf cKO males sired up to two litters but became infertile due to collapse of spermatogenesis and loss of undifferentiated spermatogonia PMID: 26831079
    16. Our results reveal the role of GDNF in nigrostriatal dopamine system postnatal development and adult function, and highlight the importance of correct spatial expression of GDNF PMID: 26681446
    17. Mice overexpressing GDNF had significantly reduced P62 protein levels suggestive of accelerated autophagy. They also had reduced PPAR-gamma and CD36 gene expression and protein levels, and lower expression of mRNA coding for enzymes involved lipogenesis. PMID: 26564715
    18. This study demonstrated that decrease in mRNA level of GDNF in brain in microgravity. PMID: 25944479
    19. GDNF signaling in the urogenital sinus increases proliferation. PMID: 25549043
    20. Results show that in the inflamed intestine, smooth muscle proliferation supports the enteric nervous system , and thus its own re-innervation, by expression of GDNF PMID: 25655216
    21. Parkin and the RET signaling cascade converge to control mitochondrial integrity and thereby properly maintain substantia nigra pars compacta dopaminergic neurons and their innervation in the striatum. PMID: 25822020
    22. expression induced by testosterone in peritubular myoid cells PMID: 25181385
    23. These data suggest that there are significant changes in the responses to GDNF as spermatogonial stem cells give rise to progenitor spermatogonia. PMID: 25165119
    24. The transcriptional start site and the promoter activity of the 5'-flanking region of GDNF have been identified. PMID: 25025809
    25. GDNF expression was upregulated upon radiation therapy in submandibular glands. PMID: 25036711
    26. This hypothesis offers a dramatically different explanation for the etiology of sporadic Parkinson's disease as a manifestation of acquired resistance to GDNF. PMID: 25448159
    27. Study highlights role of GDNF on striatal organization, both on DARPP-32-positive neurons and tyrosine hydroxylase (TH)-positive innervation; and importance of GDNF expression in striatum for TH-positive neuron viability when connected to striatum PMID: 24726488
    28. A role is found for proGDNF signaling in brains from aging and Parkinson's disease animal models. PMID: 23934644
    29. The data suggest that genetically defined cross-talk between neurotrophic factors and the brain serotonin system underlies the variability in behavioral response to GDNF. PMID: 24105724
    30. These findings suggest that pleiotropic function of GDNF is at least in part governed by modulating levels of intracellular activation of GDNF downstream pathways PMID: 24107967
    31. we show that GDNF is capable of promoting survival of 30% sympathetic precursors dissociated at embryonic day 13 PMID: 23426908
    32. GDNF is required for neural colonization of the pancreas. PMID: 23903190
    33. studies further support that long-term changes related to the dysfunction of the nigrostriatal pathway are influenced by GDNF expression PMID: 23290934
    34. injection of GL261 mouse glioma cells with GDNF knockdown by shRNA into mouse brains resulted in reduced tumor expansion and improved survival as compared to injection of control cells. PMID: 23344256
    35. SorLA acts as sorting receptor for the GDNF/GFRalpha1 complex, directing it from the cell surface to endosomes. PMID: 23333276
    36. Neuroprotective effects of carotid body transplantation in an MPTP model of Parkinson disease depends on the level of GDNF expression. PMID: 22743091
    37. spermatogonial stem cell chemotactic factor in vitro and in vivo PMID: 23040482
    38. analysis of neuroprotection by GDNF in the ischemic brain [review] PMID: 22670840
    39. This tsudy demonistrated the role of GDNF for embryonic Schwann cells migration. PMID: 22863354
    40. Targeted overexpression of GDNF in the muscles of Myo-GDNF mice did not improve motor recovery after botulinum toxin type A chemodenervation. PMID: 22473739
    41. This study identifies a regulator of midline crossing and reveals interplays between Semaphorin and gdnf signaling during axon guidance. PMID: 22998873
    42. demonstrate functionally antagonistic eicosanoid receptor subtype regulation of innate immunity-induced astrocyte GDNF expression PMID: 22499581
    43. GDNF, besides its crucial role in the self-renewal of stem cells also functions in the differentiation of chained undifferentiated spermatogonia. PMID: 22143971
    44. These results provide the first direct proof that GDNF acutely regulates the number of spermatogonial stem cells in the normal adult testis. PMID: 22232066
    45. A AT rich region in the GDNF 3' UTR have a suppressive role its gene expression. PMID: 22248285
    46. we suggest that NANOS2 acts downstream of GDNF signaling to maintain undifferentiated state of spermatogonial stem cells. PMID: 22102605
    47. These data provide direct evidence of regionally defined patch-like GDNF-positive signal. PMID: 22174794
    48. ETV5 was found to be a critical downstream regulator of GDNF signaling that mediated the expression of several known spermatogonial stem cell self-renewal related genes, including Bcl6b and LIM homeobox. PMID: 21816850
    49. Addition of motor neurons to skeletal muscle cells in vitro reduces the secretion of GDNF by skeletal muscle. PMID: 21420941
    50. In this article showed that GDNF production in mouse striatum develops in early postnatal life and is essentially confined to neurons. PMID: 22262884

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    TGF-beta family, GDNF subfamily
  • 组织特异性：
    Expressed in both the central nervous system (CNS) and in non-CNS tissues. Expressed in a highly dynamic pattern in the anterior neuroectoderm during the early stages of neurogenesis between 7.5 dpc and 10.5 dpc. Beginning at 10.5 dpc, expression begins i
  • 数据库链接：

    KEGG: mmu:14573

    STRING: 10090.ENSMUSP00000022744

    UniGene: Mm.4679