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  4. Recombinant Mouse Gigaxonin (Gan)

Recombinant Mouse Gigaxonin (Gan)

  • 货号：
    CSB-YP009228MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP009228MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP009228MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP009228MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP009228MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Gan
  • Uniprot No.：
  • 别名：
    Gan; Gigaxonin
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-597
  • 氨基酸序列
    MAEGSAVSDP QHAARLLRAL SSFREEARFC DAHLVLDGEE IPVQKNILAA ASPYIRTKLN YNPPKDDGST YKIELEGISV MVMREILDYI FSGQIRLNED TIQDVVQAAD LLLLTDLKTL CCEFLEGCIA AENCIGIRDF ALHYCLHHVH YLATEYLETH FRDVSSTEEF LELSPQKLKE VISLEKLNVG NERYVFEAVI RWIAHDVEMR KVHMKDVMSA LWVSGLDSSY LREQMLNEPL VREIVKECSN IPLSQPQQGE AMLASFKPRG YSECIVTIGG EERVSRKPTA AMRCMCPLYD PNRQLWIELA PLSMPRINHG VLSAEGFLFV LGGQDENKQT LSSGEKYDPD ANTWTALPPM HEARHNFGIV EIDGMLYILG GEDGDRELIS MECYDIYSKT WTKQPDLTMV RKIGCYAAMK KKIYAMGGGS YGKLFESVEC YDPRTQQWTA ICPLKERRFG AVACGVAMEL YVFGGVRSRE DIQGSEMVTC KSEFYHDEFK RWIYLNDQNL CIPASSSFVY GAVPIGASIY VIGDLDTGTN YDYVREFKRS TGTWHHTKPL LPSDLRRTGC AALRIANCKL FRLQLQQGLF RIRVHSP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Probable cytoskeletal component that directly or indirectly plays an important role in neurofilament architecture. May act as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Controls degradation of TBCB. Controls degradation of MAP1B and MAP1S, and is critical for neuronal maintenance and survival.
  • 基因功能参考文献：
    1. We demonstrate that gigaxonin is crucial for ubiquitin-proteasomal degradation of neuronal Intermediate filaments. Moreover,Intermediate filaments accumulation impairs mitochondrial motility and is associated with metabolic and oxidative stress PMID: 27000625
    2. gigaxonin is a major factor in the degradation of cytoskeletal intermediate filaments PMID: 23585478
    3. GAN variants are identified in brain sections of mutant mice with giant axonal neuropathy. PMID: 19782434
    4. results identify gigaxonin as a ubiquitin scaffolding protein that controls MAP1B-LC degradation, and provide insight into the molecular mechanisms underlying human neurodegenerative disorders PMID: 16227972
    5. Disruption of gigaxonin results in an impaired ubiquitin-proteasome system leading to a substantial accumulation of toxic microtubule-associated protein MAP8 in the null mutants. PMID: 16565160
    6. At 6 months of age the Gigaxonin-knockout(Deltaexon1;Deltaexon1) mice exhibit a modest hind limb muscle atrophy, a 10% decrease of muscle innervation and a 27% axonal loss in the L5 ventral roots. PMID: 18680552

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  • 亚细胞定位：
    Cytoplasm. Cytoplasm, cytoskeleton.
  • 组织特异性：
    Expressed in brain, heart and muscle (at protein level).
  • 数据库链接：

    KEGG: mmu:209239

    STRING: 10090.ENSMUSP00000070168

    UniGene: Mm.132992