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  4. Recombinant Mouse Geminin (Gmnn)

Recombinant Mouse Geminin (Gmnn)

  • 货号：
    CSB-YP009577MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP009577MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP009577MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP009577MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP009577MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Gmnn
  • Uniprot No.：
  • 别名：
    GmnnGeminin
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-206
  • 氨基酸序列
    MNLSMKQKQE GAQENVKNSP VPRRTLKMIQ PSADGSLVGR ENELPKGLFK RKLWDDQLAS QTSSCGPEAN ENKDVGDLTQ EAFDLISKEN PSSQYWKEVA EQRRKALYEA LKENEKLHKE IEQKDSEIAR LRKENKDLAE VAEHVQYMAE VIERLSNEPL DNFESPDSQE FDSEEEAVEY SELEDSGAGT CAEETVSSST DARPCT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Inhibits DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle. Inhibits histone acetyltransferase activity of KAT7/HBO1 in a CDT1-dependent manner, inhibiting histone H4 acetylation and DNA replication licensing. Inhibits the transcriptional activity of a subset of Hox proteins, enrolling them in cell proliferative control.
  • 基因功能参考文献：
    1. Geminin plays important roles in pre-meiotic DNA replication and subsequent spermatogenesis. PMID: 28690291
    2. Lack of Geminin promotes adult neural stem cell commitment toward the oligodendrocytic lineage at the expense of the neuronal differentiation without altering their ability to self-renew. PMID: 28557659
    3. These results provide proof-of-principle that preventing geminin function could prevent malignancy in tumors derived from pluripotent cells by selectively eliminating the progenitor cells with little harm to normal cells. PMID: 27821018
    4. cell penetrating (CP) Geminin is imported into the nucleus after incorporation and also the incorporated CP-Geminin directly interacted with Cdt1 or Brahma/Brg1 as the same manner as Geminin PMID: 27195810
    5. Study shows that ablation of Geminin induces massive rereplication as a result of unrestrained Cdt1 activity in embryonic stem cells, whereas it has no such effect in embryonic fibroblasts in which alternative regulation of Cdt1 activity is intact. PMID: 28296014
    6. Maternal geminin does not regulate oogenesis and oocyte meiotic maturation, but it does control accurate DNA replication and timely cleavage of fertilized eggs. PMID: 26764091
    7. Regulation of gene expression by geminin occurs only after pluripotent cells differentiate into cells in which geminin is not essential for viability. PMID: 26140583
    8. Geminin is an important regulator of self-renewal and survival of enteric nervous system progenitor cells. PMID: 26658318
    9. geminin is indispensable for fetal hematopoiesis and regulates the generation of a physiological pool of stem and progenitor cells in the fetal hematopoietic system. PMID: 25516969
    10. these data demonstrate a requirement for Geminin for neural tube patterning and neuronal differentiation during mammalian neurulation in vivo. PMID: 24995796
    11. geminin is required for Sox2 expression, and thus for the maintenance of totipotency, pluripotency and the early neural lineage. PMID: 24069236
    12. geminin acts both like a component of the FGF4 signal transduction pathway that governs trophoblast proliferation and differentiation, and geminin is required to maintain endocycles. PMID: 24412371
    13. Data indicate that geminin supported neural differentiation. PMID: 23249188
    14. Geminin dysregulation may be restored by derepressed Hoxb4 and Hoxa9 in Scmh1-deficient mice. PMID: 23207902
    15. Geminin is required during preimplantation development. Geminin knockdown inhibited the epithelial to mesenchymal transition via its ability to affect Wnt signaling and E-cadherin expression. PMID: 22335560
    16. Geminin promotes the neuronal precursor cell state by modulating both the epigenetic status and expression of genes encoding neurogenesis-promoting factors. PMID: 22949506
    17. Geminin is absolutely required for mitotic proliferation of spermatogonia but does not regulate their differentiation. PMID: 22898305
    18. Geminin regulates cortical progenitor proliferation and differentiation PMID: 21681860
    19. Geminin has been shown to coordinate proliferation and differentiation by regulating cell cycle progression, chromatin organization, and transcription in the nervous system. (Review) PMID: 21740351
    20. Idas as a novel Geminin binding partner, implicated in cell cycle progression, and a putative regulator of proliferation-differentiation decisions during development. PMID: 21543332
    21. Geminin is largely dispensable for most of embryonic and adult mammalian neurogenesis. PMID: 21408022
    22. Data suggest that transduced Hoxb4 down-regulates Geminin protein probably by constituting the E3 ubiquitin ligase for Geminin to provide hematopoietic stem and progenitor cells with proliferation potential. PMID: 21098278
    23. role for Geminin as a cell intrinsic regulator of neural fate acquisition that promotes expression of neural genes by regulating chromatin accessibility and histone acetylation. PMID: 21300881
    24. CD34(+) Gmnn(-/-) leukocyte precursors displayed DNA overreplication and formed extremely small granulocyte and monocyte colonies in methylcellulose. PMID: 21041951
    25. Data indicate that T cells deficient in Geminin show a reduced ability to repopulate lymphopenic hosts in vivo. PMID: 20107189
    26. role in inhibiting Cdt1-MCM6 interactions as well as a novel intrinsic Cdt1 DNA binding activity PMID: 12192004
    27. murine geminin associates transiently with members of the Hox-repressing polycomb complex, with the chromatin of Hox regulatory DNA elements and with Hox proteins PMID: 14973489
    28. geminin does not have a role in Cdk-phosphorylation/negative regulation of Cdt1 PMID: 14993212
    29. Data show that geminin is regulated transcriptionally by the retinoblastoma tumor suppressor (RB)/E2F pathway PMID: 15084580
    30. crystal structure of the mouse geminin-Cdt1 complex using tGeminin (residues 79-157, truncated geminin) and tCdt1 (residues 172-368, truncated Cdt1) PMID: 15286659
    31. Geminin is also down-regulated in wild-type blastocysts during S and gap-like phases by proteasome-mediated degradation, suggesting that loss of geminin is part of the mechanism regulating endoreduplication. PMID: 16847348
    32. geminin is essential for cooperative progression of the cell cycle through S phase to M phase during the preimplantation stage of development PMID: 17054725
    33. Geminin is present from S to M phase in the cell cycle PMID: 17533120
    34. These results suggested that, at least in vitro, oleic acid-containing cell membranes of the lipid bilayer inhibit Cdt1-geminin complex formation by binding to Cdt1 and thereby liberating Cdt1 from inhibition by geminin. PMID: 18288374
    35. PcG complex 1, involving Rae28 and Cdt1, supports the activity of hematopoietic stem cells by enhancing cycling capability and hematopoietic activity through direct regulation of Geminin PMID: 18650381
    36. Hepatitis B virus X protein increases the Cdt1-to-geminin ratio inducing DNA re-replication and polyploidy PMID: 18693245
    37. TIPT2 synergizes with geminin and TBP in the activation of TATA box-containing promoters. PMID: 19515240
    38. Geminin, which interacts with HOXD13 as well, blocks HOXD13-mediated assembly of pre-replication complex proteins and inhibits HOXD13-induced DNA replication. PMID: 19703996

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Geminin family
  • 数据库链接：

    KEGG: mmu:57441

    STRING: 10090.ENSMUSP00000006898

    UniGene: Mm.12239