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  4. Recombinant Mouse Gelsolin (Gsn)

Recombinant Mouse Gelsolin (Gsn)

  • 货号：
    CSB-YP009965MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP009965MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP009965MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP009965MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP009965MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Gsn; GsbGelsolin; Actin-depolymerizing factor; ADF; Brevin
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    26-780
  • 氨基酸序列
    ATTSR GRAQERAPQS RVSEARPSTM VVEHPEFLKA GKEPGLQIWR VEKFDLVPVP PNLYGDFFTG DAYVILKTVQ LRNGNLQYDL HYWLGNECSQ DESGAAAIFT VQLDDYLNGR AVQHREVQGF ESSTFSGYFK SGLKYKKGGV ASGFKHVVPN EVVVQRLFQV KGRRVVRATE VPVSWDSFNN GDCFILDLGN NIYQWCGSGS NKFERLKATQ VSKGIRDNER SGRAQVHVSE EGGEPEAMLQ VLGPKPALPE GTEDTAKEDA ANRRLAKLYK VSNGAGSMSV SLVADENPFA QGALRSEDCF ILDHGRDGKI FVWKGKQANM EERKAALKTA SDFISKMQYP RQTQVSVLPE GGETPLFKQF FKNWRDPDQT DGPGLGYLSS HIANVERVPF DAATLHTSTA MAAQHGMDDD GTGQKQIWRI EGSNKVPVDP ATYGQFYGGD SYIILYNYRH GGRQGQIIYN WQGAQSTQDE VAASAILTAQ LDEELGGTPV QSRVVQGKEP AHLMSLFGGK PMIIYKGGTS RDGGQTAPAS IRLFQVRASS SGATRAVEVM PKSGALNSND AFVLKTPSAA YLWVGAGASE AEKTGAQELL KVLRSQHVQV EEGSEPDAFW EALGGKTAYR TSPRLKDKKM DAHPPRLFAC SNRIGRFVIE EVPGELMQED LATDDVMLLD TWDQVFVWVG KDSQEEEKTE ALTSAKRYIE TDPANRDRRT PITVVRQGFE PPSFVGWFLG WDDNYWSVDP LDRALAELAA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

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靶点详情

  • 功能：
    Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
  • 基因功能参考文献：
    1. Induction of cell stress alters the actin cytoskeleton in intestinal epithelial cells via changes in the actin-binding proteins villin-1 and gelsolin. PMID: 29274870
    2. Myelin loss and subsequent axon degeneration contributes to a variety of neurological diseases, such as multiple sclerosis (MS). PMID: 28193690
    3. free actin likely contributes to impaired host defense by blocking scavenger receptor binding of bacteria. PMID: 28385809
    4. gelsolin plays a critical role in airway smooth muscle relaxation. PMID: 28118027
    5. We conclude that a nanobody-based gene therapy using adeno-associated viruses shows great potential as a novel strategy in gelsolin amyloidosis and potentially other amyloid diseases. PMID: 28334940
    6. The data presented here reveal an additional level of interplay between CCT and actin mediated via gelsolin, suggesting that CCT may influence processes depending on gelsolin activity, such as cell motility. PMID: 26364302
    7. novel roles for actin-depolymerizing factor and cofilin-1 in regulating the remodeling and permeability of epithelial junctions PMID: 26878213
    8. The data of this study identify overlapping functions for ADF and n-cofilin in presynaptic physiology and vesicle in mice. trafficking. PMID: 24770705
    9. GSNs might play a regulatory role in the suppression of the tissue damage induced by acute radiation exposure PMID: 25164111
    10. Influence of gelsolin deficiency on excitation contraction coupling in adult murine cardiomyocytes PMID: 26084219
    11. our data demonstrate that a cell-autonomous program consisting of a stress-induced Atf3-gelsolin cascade affects the change in dendritic spine morphology in models of tuberous sclerosis complex PMID: 26224859
    12. Plasma gelsolin levels decrease in the blood of mice with type II diabetes. PMID: 25478578
    13. The actin-binding proteins eps8 and gelsolin have complementary roles in regulating the growth and stability of mechanosensory hair bundles of mammalian cochlear outer hair cells. PMID: 24475274
    14. Burn-induced cerebral gelsolin expression is involved in the activation of both monocytes and astroglial cells, thereby playing a crucial role in the subsequent inflammation-induced neural apoptosis following burn injury. PMID: 23079629
    15. study concludes that cell adhesion to collagen presented on beads activates Ca(2 ) entry and promotes the formation of phagosomes enriched with NMMIIA and gelsolin PMID: 23325791
    16. The F-actin severing activity of ADF/Co fi lin organizes space for the protrusion and bundling of microtubules, the backbone of neurites. ADF/Co fi lin organizes the cytoskeleton to drive actin retrograde fl ow and thus break the spherical shape of neurons. PMID: 23259946
    17. Gelsolin deficiency alters the equilibrium between erythrocyte actin polymerization and depolymerization, causing impaired terminal maturation. PMID: 22271892
    18. data support a mechanistic model for gelsolin's role in insulin exocytosis: gelsolin clamps unsolicited soluble N-ethylmaleimide-sensitive factor attachment receptor-regulated exocytosis through association with Syn4 which is relieved upon stimulus-induced calcium influx to activate gelsolin to facilitate insulin exocytosis PMID: 22108804
    19. The ablation of actin-depolymerizing factor did not change cell death progression. PMID: 22139132
    20. Gsn knockdown suppressed expression of lipogenic genes but strikingly increased that of Tnfalpha and Il6, which may have connections with lipolytic capacity of these genes. PMID: 22445754
    21. gelsolin and NM myosin IIA interact at collagen adhesion sites to enable NM myosin IIA filament assembly and localized, Ca(2+)-dependent remodeling of actin at the nascent phagosome PMID: 21828045
    22. Data show that dendritic cells form podosomes independently of gelsolin, that there are no major alterations in their dynamics of formation and disassembly, and that they exhibit matrix-degrading function. PMID: 21779330
    23. identify the actin filament remodeling protein gelsolin as a CCT-binding partner, and although it does not behave as a classical folding substrate, gelsolin binds to the molecular chaperone CCT with a degree of specificity PMID: 20890741
    24. Gelsolin was identified as the additional interacting partners to p55 in the whirlin interactome of hair cell stereocilia. PMID: 20661277
    25. Granzyme B-induced and caspase 3-dependent cleavage of gelsolin by mouse cytotoxic T cells modifies cytoskeleton dynamics PMID: 20395300
    26. Gelsolin is necessary for macrophage motility in culture, and its absence is likely to impair the recruitment of macrophages to the injury site. PMID: 20014276
    27. Increased hippocampal neurogenesis in Gsn(-/-) mice was associated with a special microenvironment characterized by enhanced density of perfused vessels, increased regional cerebral blood flow, and increased NOS-III. PMID: 20203201
    28. explores the structure-function relationship of the gelsolin-PPI interaction PMID: 12270712
    29. Results suggest that proline-rich tyrosine kinase 2 (PYK2) is a regulator of gelsolin, revealing a novel PYK2-gelsolin pathway in regulating actin cytoskeletal organization in multiple cells, including osteoclasts. PMID: 12578912
    30. Deficiency of gelsolin in mice affects pulmonary vascular permeability and ischemic injury. PMID: 12654637
    31. Ability of gelsolin to remodel actin filaments is important for collagen-induced calcium entry PMID: 14617805
    32. Actin remodeling by endogenous gelsolin or analogues protects neurons from apoptosis mediated by mitochondria and caspase-3. PMID: 14962741
    33. concluded that gelsolin is the primary Ca2+-sensitive actin filament recycling protein in the cell and is capable of enhancing Listeria actin tail disassembly at normal resting [Ca2+]i PMID: 15671163
    34. separate functions of gelsolin mediate sequential stages of collagen phagocytosis: Ca2+-dependent actin severing facilitates collagen binding, whereas PI(4,5)P2-dependent regulation of gelsolin promotes the actin assembly PMID: 16120646
    35. Results suggest that the spatiotemporal upregulation of gelsolin in the hippocampus is induced by entorhinal deafferentation. PMID: 16261560
    36. Gelsolin exerts an overall anti-apoptotic effect in vivo. PMID: 16507893
    37. Gelsolin plays a pro-survival and apoptotic role in pancreatic beta-cells. PMID: 17192468
    38. PIP5KIgamma-mediated generation of phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) at sites of N-cadherin contacts regulates intercellular adhesion strength, an effect due in part to PI(4,5)P2-mediated regulation of gelsolin. PMID: 17538019
    39. GSN was up-regulated in the airways upon ventilator-induced lung injury. Genetic ablation led to protection. GSN fragmentation was an effector mechanism in VILI-induced apoptosis. GSN expression was necessary for efficient neutrophil infiltration PMID: 19202007
    40. Gelsolin regulates cardiac remodeling after myocardial infarction through DNase I-mediated apoptosis. PMID: 19246681

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  • 亚细胞定位：
    [Isoform 2]: Cytoplasm, cytoskeleton.; [Isoform 1]: Secreted.
  • 蛋白家族：
    Villin/gelsolin family
  • 数据库链接：

    KEGG: mmu:227753

    STRING: 10090.ENSMUSP00000028239

    UniGene: Mm.21109