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  4. Recombinant Mouse GTPase HRas (Hras1)

Recombinant Mouse GTPase HRas (Hras1)

  • 货号：
    CSB-YP730737MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP730737MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP730737MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP730737MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP730737MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Hras; Hras1; GTPase HRas; H-Ras-1; Transforming protein p21; c-H-ras; p21ras) [Cleaved into: GTPase HRas; N-terminally processed]
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-186
  • 氨基酸序列
    MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHQYREQI KRVKDSDDVP MVLVGNKCDL AARTVESRQA QDLARSYGIP YIETSAKTRQ GVEDAFYTLV REIRQHKLRK LNPPDESGPG CMSCKC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
  • 基因功能参考文献：
    1. gene expression profiles of each of the Ras isoforms in a panel of mouse tissues derived from a full developmental time course, are reported. PMID: 28117393
    2. This study demonstrates that H- ras deletion protects against AngII-induced cardiac remodeling, possibly via a mechanism in which PKG-Ibeta overexpression could play a partial role, and points to H-Ras and/or downstream proteins as potential therapeutic targets in cardiovascular disease. PMID: 29054855
    3. HRas (G12V/G12V) mice showed robust upregulation of ERK signaling, neuronal hypertrophy, increased brain volume, spatial learning deficits, and impaired mGluR-dependent long-term depression (LTD). PMID: 28455524
    4. the oncogenic Hras mutation modulates energy homeostasis in vivo. PMID: 29254681
    5. Loss of wild-type Hras promotes the earliest stages of pancreatic tumorigenesis, and moreover results in more rapid progression of the disease. As such, mechanisms leading to activation of wild-type Ras proteins, including but not limited to redox-dependent reactions, may influence the development of pancreatic cancer. PMID: 28162272
    6. High HRAS expression is associated with hepatocarcinogenesis. PMID: 28481866
    7. this study shows that retinoic acid stabilizes HRas protein during neurogenesis. PMID: 27185863
    8. we provide genetic evidence that the wild-type H-Ras and K-Ras proteins are bioequivalent in spite of their different structural and biological properties PMID: 27872088
    9. loss of one allele of Hras increased the sensitivity of mice to this carcinogen, and this effect was further exacerbated by the loss of the second Hras allele. However, loss of one or both alleles of Nras failed to alter tumor burden, either in the absence or presence of Hras, after exposure to urethane. PMID: 27911940
    10. H-ras isoform mediates protection against pressure overload-induced cardiac dysfunction in part through activation of AKT/PI3K signaling pathway. PMID: 28193718
    11. The long intergenic non-coding RNA CCR492 functions as a let-7 competitive endogenous RNA to de-repress c-Myc expression and to promote cell transformation assisted by the constitutively active H-Ras. PMID: 27344374
    12. these contrasting signatures precisely match those proposed to confer bias toward Hras(CAA61CTA) versus Braf(GTG636GAG) mutations in the original tumor sets. Our findings highlight a novel mechanism whereby exposure history acts through strand-biased mutagenesis to specify activation of preferred oncogenes PMID: 27207659
    13. We find that the tumor suppressive effects of Hras are nullified in a homozygous mutant p53 background. As such, loss of wild-type Hras fosters the earliest stages of pancreatic cancer in a p53-dependent manner. PMID: 26452271
    14. This study establishes a role for the loss of microRNA-203 in promoting selection and expansion of Hras mutated cells and identifies a mechanism through which microRNA-203 antagonizes Hras-mediated tumorigenesis. PMID: 26203562
    15. The data suggest a role for redox-dependent activation of wild-type KRAS through cysteine 118 in oncogenic HRAS-driven tumorigenesis. PMID: 25902334
    16. Phenotypic Screening Identifies Protein Synthesis Inhibitors as H-Ras-Nanocluster-Increasing Tumor Growth Inducers. PMID: 26568031
    17. H-Ras mediates the inhibitory effect of epidermal growth factor on the epithelial Na+ channel PMID: 25774517
    18. Conditional inactivation of p53 in urothelial cells of transgenic mice expressing mutant HRAS results in carcinoma in situ and basal-subtype muscle-invasive urothelial carcinomas of the bladder with focal squamous differentiation. PMID: 25795707
    19. Hras allelic imbalance is an obligate step in papilloma development and occurs only at the papilloma stage. PMID: 24240680
    20. the Arf-Egr-C/EBPbeta axis as an important determinant of cellular responses (senescence or transformation) to oncogenic Ras signaling PMID: 25535333
    21. MicroRNA 17-92 cluster mediates ETS1 and ETS2-dependent RAS-oncogenic transformation PMID: 24968297
    22. CARD9 regulates H-Ras activation by linking Ras-GRF1 to H-Ras, which mediates Dectin-1-induced extracellular signal-regulated protein kinase (ERK) activation and proinflammatory responses when stimulated by their ligands. PMID: 25267792
    23. Data show that peroxisome proliferator-activated receptor-beta/delta (PPARbeta/delta) promotes HRAS-induced senescence to inhibit tumorigenesis by potentiating p-ERK1/2 and repressing p-AKT signaling. PMID: 24213576
    24. the function of the Ras pathway and its many effectors, including MAPK and PI3K, in the teeth of mice PMID: 24057668
    25. These observations provide genetic evidence for an essential role of Ras proteins in the control of keratinocyte and epidermal proliferation. PMID: 23831572
    26. Computational modeling revealed that complexes containing H-ras conformers and galectin-1 affect both the number and lifetime of nanoclusters and thus determine the specific Raf effector recruitment. PMID: 24569991
    27. Positive feedback between PI3K and HRas is essential for fibroblasts to spontaneously self-organize and generate a productive migratory response in the absence of spatial cues. PMID: 23676667
    28. Data indicate that Hras heterozygosity increases the rate of malignant progression of skin tumors. PMID: 22945650
    29. we induced tumors in mice via the injection of transposons encoding three oncogenes(HRAS, c-Myc and shp53) and a plasmid-expressing transposase. PMID: 23380875
    30. Results suggest L1-mediated signaling by the L1-ezrin-NF-kappaB pathway, that induces IGFBP-2 expression, has an important role in colorectal cancer progression. PMID: 22847612
    31. These results show that CD8(+) T cells can orchestrate skin inflammation with psoriasis-like pathology in response to constitutive RAS activation in keratinocytes, and this is primarily mediated through IFN-gamma. PMID: 23151849
    32. Oxidative stress differentially regulates the expression of Ha-Ras and Ki-Ras in cultured astrocytes. PMID: 23213349
    33. ASPP2 modulates oncogenic RAS-induced autophagic activity to dictate the cellular response to RAS: to proliferate or senesce. PMID: 22847423
    34. The findings revealed a novel mechanism by which PEA-15 positively regulates Ras/ERK signaling and increases the proliferation of H-Ras-transformed epithelial cells through enhanced phospholipase D1 expression and activation. PMID: 22105357
    35. Upregulation of NKG2D ligands by H-RasV12 increased sensitivity of cells to NK cell-mediated cytotoxicity PMID: 22798674
    36. H-Ras isoform is responsible for extracellular matrix synthesis, proliferation, and migration in fibroblasts. PMID: 22094331
    37. disruption of the RP-Mdm2-p53 pathway by an Mdm2(C305F) mutation does not accelerate prostatic tumorigenesis induced by inactivation of the pRb family proteins (pRb/p107/p130) PMID: 21747916
    38. these results suggest that the expression of H-ras12V oncogene leads to elevated levels of ROS and apolipoprotein A-I that contribute to steatosis. PMID: 21600874
    39. we demonstrate for the first time that H-ras and N-ras act as critical controllers of Th1 responses PMID: 21444916
    40. the activation of H-ras and N-ras isoforms does not play a major role in the early renal damage induced by unilateral ureteral obstruction PMID: 19288266
    41. The activated CDK4 cooperates with the oncogenic HRAS(G12V) protein to increase the susceptibility of melanoma development in vivo.(Hras protein) PMID: 20703083
    42. H-ras(G12V) expression also accelerated the increase following MD in the frequency of miniature excitatory potentials, mirroring accelerated plasticity in vivo. PMID: 20937865
    43. Data suggest that constantly high Ras activity in differentiated neurons downregulates hippocampal precursor cell generation in the neuronal lineage, but is modulated by sex-dependent factors. PMID: 20398060
    44. Data show that p66(Shc) coordinates Ras-dependent control of proliferation and anchorage sensation. PMID: 20676142
    45. Ha-ras oncogene regulates morphogenesis, tumorigenesis, and metastasis through suppressing nm23 expression and modulation of immune cell function. PMID: 20944141
    46. H-Ras and N-Ras have distinct functional roles during initial stages of the cell cycle PMID: 19895680
    47. Ha-ras overexpression increases phosphorylation of Stat3 at serine-727 and tyrosine-705 in 293T cells and enhances oncogenicity of the cell. PMID: 19182994
    48. Studies with transgenic mice expressing a constitutively active H-Ras suggest that despite the common origin of the lens & cornea, these 2 tissues possess distinct sets of downstream targets that regulate the cellular responses to Ras activation. PMID: 20105280
    49. Using lung cell lines expressing oncogenic K-Ras, s show that NF-kappaB is activated in these cells in a K-Ras-dependent manner and that NF-kappaB activation by K-Ras. PMID: 20406971
    50. Angiotensin II-induced activation of c-Ret signaling with RAS is critical in ureteric bud branching morphogenesis PMID: 19961928

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  • 亚细胞定位：
    Cell membrane. Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus. Golgi apparatus membrane; Lipid-anchor.
  • 蛋白家族：
    Small GTPase superfamily, Ras family
  • 数据库链接：

    KEGG: mmu:15461

    STRING: 10090.ENSMUSP00000026572

    UniGene: Mm.334313