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  4. Recombinant Mouse GDH/6PGL endoplasmic bifunctional protein (H6pd)

Recombinant Mouse GDH/6PGL endoplasmic bifunctional protein (H6pd)

  • 中文名称：
    小鼠H6pd重组蛋白
  • 货号：
    CSB-YP805580MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠H6pd重组蛋白
  • 货号：
    CSB-EP805580MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠H6pd重组蛋白
  • 货号：
    CSB-EP805580MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠H6pd重组蛋白
  • 货号：
    CSB-BP805580MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠H6pd重组蛋白
  • 货号：
    CSB-MP805580MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    H6pd
  • Uniprot No.：
  • 别名：
    H6pdGDH/6PGL endoplasmic bifunctional protein [Includes: Hexose-6-phosphate dehydrogenase; Glucose 1-dehydrogenase; EC 1.1.1.47; Glucose-6-phosphate dehydrogenase; EC 1.1.1.363); 6-phosphogluconolactonase; 6PGL; EC 3.1.1.31)]
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    17-789
  • 氨基酸序列
    QELK GHVSIILLGA TGDLAKKYLW QGLFQLYLDE AGKGHSFSFH GAALTAPQQG QKLMDKVLES LSCPKDLVPS RCDELKGQFL QLSQYRQLKT VEDYQTLNKD IETQVQQDGL WEAGRIFYFS VPPFAYADIA RNINSSCRPH PGAWLRVVFE KPFGHDHLSA QQLASELGSF FQEEEMYRVD HYLGKQAVAQ ILPFRDQNRK ALDGLWNRHH VERVEIILKE TIDAEGRASF YEEYGVIRDT LQNHLTEILT LVAMELPLNI SSSAAVLQHK LWAFQALRGL QKSSAILGQY QAYSGQVRRE LQKPDGFQSL TPTFAGVLVH IDNLRWEGVP FILMSGKALD ERVGYVRIVF KNRAYCTQSE RHWVPEQSRC LPQQIIFYIG HGELGHPAIL VSRNLFKPSL PTQKWKEVQD QPGLRLFGRP LSDYYAYRPV REQDAYSTLL SHIFHCRKES FITTENLLAS WVFWTPLLDS LAFEVPRPYP GGAENGQLLD FEFSGGQLTF SQQQLEVLIP DLGSVPKPSD FQVLGARYRQ SPLITAWPEE LISKLASDIE AAAVQAVRHF GKFHLALSGG SSPIALFQQL ATGHYSFPWA HTHLWLVDER CVPLSDPDSN FQGLQAHLLQ HVRVPYYNIH PMPVHLHQRL CAEEDQGAQT YASEISALVA NSSFDLVLLG MGTDGHTASL FPQSPTGLDG DQLVVLTESP FRPHQRMSLS LPLINRAKKV AVLVMGRTKR EITTLVSRVG HEPKKWPISG VVPLSGQLVW YMDYEAFLG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Bifunctional enzyme localized in the lumen of the endoplasmic reticulum that catalyzes the first two steps of the oxidative branch of the pentose phosphate pathway/shunt, an alternative to glycolysis and a major source of reducing power and metabolic intermediates for biosynthetic processes. Has a hexose-6-phosphate dehydrogenase activity, with broad substrate specificity compared to glucose-6-phosphate 1-dehydrogenase/G6PD, and catalyzes the first step of the pentose phosphate pathway. In addition, acts as a 6-phosphogluconolactonase and catalyzes the second step of the pentose phosphate pathway. May have a dehydrogenase activity for alternative substrates including glucosamine 6-phosphate and glucose 6-sulfate. The main function of this enzyme is to provide reducing equivalents such as NADPH to maintain the adequate levels of reductive cofactors in the oxidizing environment of the endoplasmic reticulum. By producing NADPH that is needed by reductases of the lumen of the endoplasmic reticulum like corticosteroid 11-beta-dehydrogenase isozyme 1/HSD11B1, indirectly regulates their activity.
  • 基因功能参考文献：
    1. We envisage these data and models finding utility when investigating the muscle-specific functions of the 11beta-HSD1/G6PT/H6PDH triad. PMID: 28749080
    2. Data suggest that Gh (growth hormone) represses H6pd through locally produced Igf1 (insulin-like growth factor 1); Gh directly represses Hsd11b1 (11-beta-hydroxysteroid dehydrogenase 1) mRNA rather than acting via Igf1 receptor. PMID: 24759003
    3. These results suggest that increased H6PDH expression specifically in adipose tissue is sufficient to cause intra-adipose glucocorticoid production and adverse metabolic phenotypes. PMID: 24381005
    4. Findings suggest that increased hepatic H6PDH and G6PT production contribute to 11beta-HSD1 upregulation of local glucocorticoid action that may be related to the development of type 2 diabetes. PMID: 21052977
    5. These data are consistent with an 11beta-HSD1-independent function for H6PDH in which sarcoplasmic reticulum G6P metabolism and NAD/NADH redox status are important for maintaining muscle homeostasis. PMID: 21106871
    6. High-glucose-mediated decrease in G6PD activity may provide a mechanistic explanation for the gradual loss of beta cells in patients with diabetes. PMID: 20032314
    7. Data suggest that glucose-6-phosphate dehydrogenase (G6PD) is the only NADPH-producing enzyme activated in response to oxidative stress. PMID: 12466018
    8. Data suggest that aberrant increase of glucose-6-phosphate dehydrogenase in obese and/or diabetic subjects would alter lipid metabolism and adipocytokine expression, thereby resulting in failure of lipid homeostasis and insulin resistance in adipocytes PMID: 15923630
    9. Required for 11 beta-hydroxysteroid dehydrogenase type 1 activity PMID: 16356929
    10. Both glucose output and glucose use are abnormal in mice lacking hexose-6-phosphate dehydrogenase. PMID: 17656460
    11. Data postulate that increased glycogen synthesis may reflect increased flux of glucose-6-phosphate (H6PDH substrate) through to glycogen in the absence of hexose-6-phosphate dehydrogenase mediated metabolism. PMID: 17823265
    12. Lack of hexose-6-phosphate dehydrogenase impairs lipid mobilization from mouse adipose tissue. PMID: 18218694
    13. absence of H6PD induces a progressive myopathy by altering the sarcoplasmic reticulum redox state PMID: 18222920

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  • 亚细胞定位：
    Endoplasmic reticulum lumen.
  • 蛋白家族：
    Glucose-6-phosphate dehydrogenase family; Glucosamine/galactosamine-6-phosphate isomerase family, 6-phosphogluconolactonase subfamily
  • 组织特异性：
    Expressed in liver (at protein level). Expressed in muscles. Expressed in adipose tissues.
  • 数据库链接：

    KEGG: mmu:100198

    STRING: 10090.ENSMUSP00000030830

    UniGene: Mm.22183