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  4. Recombinant Mouse G1/S-specific cyclin-D1 (Ccnd1)

Recombinant Mouse G1/S-specific cyclin-D1 (Ccnd1)

  • 货号：
    CSB-YP004811MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP004811MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP004811MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP004811MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP004811MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Ccnd1; Cyl-1G1/S-specific cyclin-D1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-295
  • 氨基酸序列
    MEHQLLCCEV ETIRRAYPDT NLLNDRVLRA MLKTEETCAP SVSYFKCVQK EIVPSMRKIV ATWMLEVCEE QKCEEEVFPL AMNYLDRFLS LEPLKKSRLQ LLGATCMFVA SKMKETIPLT AEKLCIYTDN SIRPEELLQM ELLLVNKLKW NLAAMTPHDF IEHFLSKMPE ADENKQTIRK HAQTFVALCA TDVKFISNPP SMVAAGSVVA AMQGLNLGSP NNFLSCYRTT HFLSRVIKCD PDCLRACQEQ IEALLESSLR QAQQNVDPKA TEEEGEVEEE AGLACTPTDV RDVDI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner.
  • 基因功能参考文献：
    1. autophagic degradation machinery and cyclin D1 linked to liver tumorigenesis PMID: 29328502
    2. Treatment of mice with established BCL1 leukemia using the scFv-targeted polymer conjugate leads to a markedly prolonged survival time of the experimental animals compared with the treatment using the free drug and the nontargeted polymer-pirarubicin conjugate. PMID: 28921867
    3. results suggest that DGKdelta controls the down-regulation of cyclin D1 expression by attenuating the PKC signaling pathway for C2C12 myogenic differentiation PMID: 29859210
    4. the focal adhesion component paxillin is a cytoplasmic substrate of Ccnd1.Cdk4. PMID: 27181366
    5. Since miR-290 cluster miRNAs are the most dominant stem-cell-specific miRNAs, our results revealed an important cause for the absence of Cyclin D1 in mouse embryonic stem cells PMID: 28510621
    6. Ablation of periostin suppresses post-infarction myocardial regeneration by inhibiting the PI3K/GSK3beta/cyclin D1 signalling pathway, indicating that periostin is essential for myocardial regeneration. PMID: 28453729
    7. Cyclin D1 is indispensable for normal hematopoiesis; in its absence, cyclins D2 and D3 are also not expressed, preventing hematopoietic cell division and differentiation at its earliest stage. The results demonstrate that not all functions of individual D cyclins are redundant, and highlight a master role of cyclin D1 in hematopoiesis. PMID: 27129404
    8. NMB or NMBR silencing inhibited M-CSF/c-Fms-mediated downstream signaling pathways like activation of ERK and Akt and induction of D-type cyclins, cyclin D1 and D2. PMID: 28780306
    9. Histone H2A T120 phosphorylation promotes oncogenic transformation via upregulation of cyclin D1. PMID: 27716482
    10. identify Pax5 and cyclin D1 as Zfp521 target genes, and suggest that excessive B-cell proliferation observed in mice with retroviral insertions near the Zfp521 gene is due to an up-regulation of cyclin D1 in B-cells. PMID: 27107743
    11. Data show that expression of the Oct-4, Sox2, Klf4, and c-Myc (OSKM) reprogramming factors induces Cyclin D1 expression, and the increased Cyclin D1 expression during reprogramming promotes continuing embryonic fibroblasts (MEFs) proliferation. PMID: 27790870
    12. study shows that PLCgamma1 controls osteoclast numbers via a CSF-1-dependent DAG/beta-catenin/cyclinD1 pathway. PMID: 27941021
    13. Regarding extratelomeric activities, our results showed a decrease of 64, 38 and 25% in the transcription of c-Myc, Cyc-D1 and TERT, respectively (p<0.05) after AZT treatment. Furthermore, we found an effect on cell migration, reaching an inhibition of 48% (p<0.05) and a significant passage-dependent increase on cell doubling time during treatment. PMID: 27633795
    14. Using Ccnd1 knockout mice, the study shows that Ccnd1 expression significantly contributes to tumor incidence in teratoma susceptible mice without being necessary for normal germ cell or testis development. PMID: 26901436
    15. Ras (G12V)-induced cyclin D1 protein synthesis was markedly suppressed by the knockdown of IL-33. PMID: 27155324
    16. Novel RNA-binding activity of MYF5 enhances Ccnd1 mRNA translation during myogenesis. PMID: 26819411
    17. The robust increase in the expression of cyclin D1 and CDK4 at day 2 suggests that Muller glia may be in G1 phase of the cell cycle at this stage. PMID: 26975029
    18. Over-expression of cyclinD1 promotes the proliferation of neural stem cells. PMID: 26162780
    19. show that the knockdown of smc1a in zebrafish impairs neural development, increases apoptosis, and specifically down-regulates Ccnd1 levels PMID: 26206533
    20. Data show that nonlinear responses were observed in lenses for EdU (5-ethynyl-2'-deoxy-uridine) incorporation, cyclin D1 staining and cell density after 24 h at 100 and 250 mGy. PMID: 25924630
    21. Cyclin D has a role in activating the Rb tumor suppressor by mono-phosphorylation PMID: 24876129
    22. Cyclin D1 is capable of transcriptionally upregulating Pax6, which promoted reprogramming cells to commit to a neural progenitor fate rather than a pluripotent cell fate PMID: 25261251
    23. Cyclin D1 binds to STAT3, a transcription factor downstream of astrogliogenic cytokines, and suppresses its transcriptional activity on the glial fibrillary acidic protein (Gfap) gene. PMID: 24302516
    24. These results reveal that GCNF plays a key role in regulating activation of cyclin D1 expression via inhibition of Mir302a. PMID: 24578347
    25. binds to the promoter region of the cell growth regulatory genes Cyclin D1 and COX-2 PMID: 23813870
    26. Two conserved MyoD-binding sites were identified in an intronic enhancer of Fra-1 and shown to be required for the stable repression of Fra-1 and, in turn, cyclin D1 PMID: 25006242
    27. The population of mature oligodendrocytes is markedly reduced in the absence of cyclin D1. PMID: 24550001
    28. These studies reveal a novel role for cyclin D1 on metabolism via PGC1alpha and reveal a potential link between cell-cycle regulation and metabolic control of glucose homeostasis. PMID: 24947365
    29. Downregulation of cyclin D1 expression is necessary for the maintenance of cell cycle exit in adult cardiomyocytes. PMID: 24821722
    30. FGF2 signaling results in the phosphorylation of Erk1/2, and activation of c-Fos and c-Jun that lead to elevated cyclin D mRNA levels. PMID: 24155149
    31. insulin activates cyclin D1-cyclin-dependent kinase 4 (Cdk4), which, in turn, increases GCN5 acetyltransferase activity and suppresses hepatic glucose production independently of cell cycle progression PMID: 24870244
    32. RanBPM-dependent Dyrk1B cytosolic retention and degradation is reverted in the presence of Cend1 resulting in cyclin D1 destabilization PMID: 24312406
    33. Induction of Dicer by cyclin D1 coordinates microRNA biogenesis in mammary gland neoplasms. PMID: 24287487
    34. Protein malnutrition suppressed cell cycle progression by downregulating cyclin D1 and overexpression p21/p27. PMID: 24290603
    35. This is the first report to show that DIF-1 inhibits tumor growth in vivo, consistent with its in-vitro action, suggesting that this compound may have potential as a novel anti-tumor agent. PMID: 24670930
    36. miR-499 regulates the proliferation and apoptosis of embryonic stem cells cells in the late stage of cardiac differentiation via its effects on Sox6 and cyclin D1. PMID: 24040263
    37. Cdk6/Ccnd1 overexpression inhibits chondrocyte maturation and enhances G1/S transition by phosphorylating pRb; the chondrocytes then undergo p53-dependent apoptosis PMID: 23624920
    38. Jianpi Bushen Prescription activates expression of the cyclin D1 gene in irradiated mice. PMID: 24114209
    39. This study reveals a pivotal role of combined Dmp1 loss and cyclin D1 overexpression in breast cancer. PMID: 23938323
    40. Cyclin D1 has a role in prostate cancer that involves coordinating restraint of epithelial-mesenchymal transition and stem cell expanding gene expression PMID: 24282282
    41. Cyclin D1 mediates the convergence of ERalpha and growth factor signaling at a common cis-element of growth factor genes. PMID: 23864650
    42. p107 retinoblastoma protein is regulated by Cdk6/Ccnd1 in growth plates. PMID: 23803733
    43. This study demonistrated that Cyclin D1 is required for proliferation of Olig2-expressing progenitor cells in the injured cerebral cortex. PMID: 23839966
    44. Our data indicate that decreasing cyclin D1 levels would be an efficient treatment for tumors induced by Notch-1 signaling PMID: 22907433
    45. Relaxin alleviated airway inflammation, airway smooth muscle thickening and airway remodelling in a murine model of chronic asthma, partially by down-regulating the expression level of cyclin D(1). PMID: 22883994
    46. PI3K signal pathway and Notch signal pathway may coordinately regulate the cell proliferation and differentiation processes through up-regulating cyclinD1 and down-regulating p27kip1 of CD4+ T lymphocytes in asthma PMID: 23557855
    47. Data indicate that selenium (Se) promoted G1-phase cell cycle progression along with induction of cyclin D1 expression at the mRNA and protein level. PMID: 22641559
    48. Acupuncture and moxibustion therapy could improve Cyclin D1 expression in mice with cyclophosphamide induced myelosuppression. PMID: 21425582
    49. A previously unrecognized function of cyclin D1 in suppressing autophagy in the mammary epithelium. PMID: 23041550
    50. SUMO modification of Stra13 is required for repression of cyclin D1 expression and cellular growth arrest PMID: 22905217

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  • 亚细胞定位：
    Nucleus. Cytoplasm. Nucleus membrane.
  • 蛋白家族：
    Cyclin family, Cyclin D subfamily
  • 数据库链接：

    KEGG: mmu:12443

    STRING: 10090.ENSMUSP00000091495

    UniGene: Mm.273049