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  4. Recombinant Mouse G protein-coupled receptor kinase 6 (Grk6)

Recombinant Mouse G protein-coupled receptor kinase 6 (Grk6)

  • 货号：
    CSB-YP009927MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP009927MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP009927MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP009927MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP009927MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Grk6; Gprk6; G protein-coupled receptor kinase 6; EC 2.7.11.16; G protein-coupled receptor kinase GRK6
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-576
  • 氨基酸序列
    MELENIVANT VLLKAREGGG GNRKGKSKKW RQMLQFPHIS QCEELRLSLE RDYHSLCERQ PIGRLLFREF CATRPELTRC TAFLDGVSEY EVTPDEKRKA CGRRLMQNFL SHTGPDLIPE VPRQLVSNCA QRLEQGPCKD LFQELTRLTH EYLSTAPFAD YLDSIYFNRF LQWKWLERQP VTKNTFRQYR VLGKGGFGEV CACQVRATGK MYACKKLEKK RIKKRKGEAM ALNEKQILEK VNSRFVVSLA YAYETKDALC LVLTLMNGGD LKFHIYHMGQ AGFPEARAVF YAAEICCGLE DLHRERIVYR DLKPENILLD DHGHIRISDL GLAVHVPEGQ TIKGRVGTVG YMAPEVVRNE RYTFSPDWWA LGCLLYEMIA GQSPFQQRKK KIKREEVERL VKEVAEEYTD RFSSQARSLC SQLLSKDPAE RLGCRGGGAR EVKEHPLFKK LNFKRLGAGM LEPPFKPDPQ AIYCKDVLDI EQFSTVKGVD LEPTDQDFYQ KFATGSVSIP WQNEMVETEC FQELNVFGLD GSVPPDLDWK GQPTAPPKKG LLQRLFSRQD CCGNCSDSEE ELPTRL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Specifically phosphorylates the activated forms of G protein-coupled receptors. Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their desensitization. Seems to be involved in the desensitization of D2-like dopamine receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor, LRP6 during Wnt signaling (in vitro).
  • 基因功能参考文献：
    1. Conditions for optimal dendritic cells guidance are perfectly provided by the CCL21 gradients measured in vivo. Furthermore, CCR7 signal termination by the G-protein-coupled receptor kinase 6 (GRK6) is crucial for haptotactic but dispensable for chemotactic CCL21 gradient sensing in vitro and confirm those observations in vivo. PMID: 28457871
    2. These studies suggest a previously unrecognized and novel role for GRK6 in neutrophil migration specific to pulmonary tissue during bacterial infection. PMID: 28939643
    3. Silencing of GRK6 expression by siRNA impairs CXCR4-serine 339 phosphorylation and migration of cardiac stem cellss caused by SCF. In vivo, knockdown of GRK6 impairs the ability of CSCs to migrate into peri-infarcted areas. PMID: 27245949
    4. This work demonstrates the importance of GRK6 in regulation of hematopoietic stem cell self-renewal and reveals its potential role in participation of stress response. PMID: 27882944
    5. GRK6-mediated OXTR desensitization in labor is necessary for normal uterine contractile patterns and optimal fetal outcome. PMID: 26886170
    6. It regulates apoptotic engulfment in phagocytes. (review) PMID: 26234352
    7. these results indicate that GRK6 complexes with AGS3-Galphai2 to regulate CXCR2-mediated leukocyte functions at different levels, including downstream effector activation, receptor trafficking, and expression at the cell membrane. PMID: 24510965
    8. data demonstrate that GRK6 plays a feedback regulatory role in CXCR2-mediated tumor development, invasion and metastasis PMID: 23589623
    9. G-protein-coupled receptor kinase 6 (GRK6) is involved in apoptotic cell clearance. PMID: 23443560
    10. Chemokine receptor CXCR2 interacts with GRK6 to negatively regulate receptor sensitization and trafficking, thus affecting cell signaling and angiogenesis. PMID: 22869904
    11. Results identify GRK5/6 as novel kinases for the single transmembrane receptor LRP6 during Wnt signaling. PMID: 19801552
    12. deficiency causes defective lymphocyte chemotaxis PMID: 12032308
    13. Postsynaptic D2-like dopamine receptors are physiological targets for GRK6 and suggests that this regulatory mechanism contributes to central dopaminergic supersensitivity. PMID: 12718862
    14. In the absence of GRK6, the G-CSF-induced increase in circulating neutrophils is profoundly impaired. After injection of pegylated-G-CSF, significantly lower numbers of circulating neutrophils were observed in GRK6-/- as compared with wild-type (WT) mice. PMID: 14704365
    15. The results suggest that mouse G-protein-coupled receptor kinase isoform 6-C may be considered a basic, prototypic representative of the GRK4-like kinases. PMID: 16302968
    16. GRK6 and -2, but not GRK5, are involved in the pathogenesis of acute arthritis in the K/BxN model. In particular, GRK6 may dampen inflammatory responses by regulating granulocyte trafficking toward chemoattractants. PMID: 18662895
    17. Data show an important protective role for GRK6 against post-inflammatory visceral hyperalgesia. PMID: 18977289
    18. these results suggest that GRK6 may play a role in regulating some, but not all morphine-mediated responses. PMID: 19497686

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  • 亚细胞定位：
    Membrane; Lipid-anchor.
  • 蛋白家族：
    Protein kinase superfamily, AGC Ser/Thr protein kinase family, GPRK subfamily
  • 组织特异性：
    Expressed in the brain in striatal neurons.
  • 数据库链接：

    KEGG: mmu:26385

    STRING: 10090.ENSMUSP00000001115

    UniGene: Mm.10193