Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Mouse Friend virus susceptibility protein 1 (Fv1)

Recombinant Mouse Friend virus susceptibility protein 1 (Fv1)

  • 货号：
    CSB-YP304889MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP304889MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP304889MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP304889MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP304889MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Fv1
  • Uniprot No.：
  • 别名：
    Fv1Friend virus susceptibility protein 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-459
  • 氨基酸序列
    MNFPRALAGF SSWLFKPELA EDSPDNDSPD NDTVNPWREL LQKINVADLP DSSFSSGKEL NDSVYHTFEH FCKIRDYDAV GELLLAFLDK VTKERDQFRD EISQLRMHIN DLKASKCVLG ETLLSYRHRI EVGEKQTEAL IVRLADVQSQ VMCQPARKVS ADKVRALIGK EWDPVTWDGD VWEDIDSEGS EEAELPTVLA SPSLSEESGY ALSKERTQQD KADAPQIQSS TSLVTSEPVT RPKSLSDLTS QKHRHTNHEL NSLAHSNRQK AKEHARKWIL RVWDNGGRLT ILDQIEFLSL GPLSLDSEFN VIARTVEDNG VKSLFDWLAE AWVQRWPTTR ELQSPDTLEW YSIEDGIERL RELGMIEWLC VKATCPQWRG PEDVPITRAM RITFVRETRE TWKSFVFSLL CIKDITVGSV AAQLHDLIEL SLKPTAAGLT SVGSVGVLSL SPWKHQSNS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Retroviral restriction factor that prevents infection by gammaretroviruses. Acts by interacting with the capsid protein ca after entry of the virus into the cell. This interaction presumably disrupt the capsid thereby inactivating the viral genome, making it unable to enter host nucleus and integrate into host genome.
  • 基因功能参考文献：
    1. Regions of Fv1 adapt cooperatively, highlighting its preference for repeated structures and suggesting that this functionally constrained aspect of the retroviral capsid lattice presents a common target in the evolution of intrinsic immunity. PMID: 30224488
    2. data indicate that for some but not all Fv1 proteins, gain of restriction activities could be achieved by increasing the expression level of Fv1; however such a concentration dependent effect is not seen with most Fv1s and cannot explain the recently reported activities against non-MLVs PMID: 27342974
    3. These results support the hypothesis that Fv1 defended mice against waves of retroviral infection PMID: 24603659
    4. The antiparallel organization of the NtD regions of Fv1 and Trim5alpha dimers correctly positions C-terminal specificity and N-terminal effector domains and facilitates stable binding to adjacent capsid hexamers in viral cores. PMID: 24979782
    5. Ordered assembly of murine leukemia virus capsid protein on lipid nanotubes directs specific binding by the restriction factor, Fv1. PMID: 21436027
    6. an examination of expression and intracellular localization of this protein PMID: 12667816
    7. A variety of amino acid changes affecting Fv1 tropism were identified, at mouse leukemia virus CA protein positions 82, 92 to 95, 105, 114, and 117, and they all were mapped to the apparent exterior of virion-associated CA. PMID: 15331691
    8. defined the time point at which Fv1 interacts with virus and shown that it can work additively and independently of TRIM5; conclude that Fv1 and TRIM5 can both recognize retroviral particles early after cell entry and before reverse transcription PMID: 16474118
    9. A model for the arrangement of domains in Fv1 suggesting that dimerization of the N-terminal domain is necessary for Fv1 function to allow the protein to interact with multiple capsid protomers in retroviral cores was proposed. PMID: 16873278
    10. Virus with deletion of 114His in the capsid gene failed to replicate in Fv1-expressing cells. PMID: 18467880

    显示更多

    收起更多

  • 数据库链接：

    KEGG: mmu:14349

    STRING: 10090.ENSMUSP00000092054

    UniGene: Mm.422607