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  4. Recombinant Mouse Four and a half LIM domains protein 2 (Fhl2)

Recombinant Mouse Four and a half LIM domains protein 2 (Fhl2)

  • 货号：
    CSB-YP008664MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP008664MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP008664MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP008664MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP008664MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Fhl2Four and a half LIM domains protein 2; FHL-2; Skeletal muscle LIM-protein 3; SLIM-3
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-279
  • 氨基酸序列
    MTERFDCHHC NESLYGKKYI LKEENPHCVA CFEELYANTC EECGTPIGCD CKDLSYKDRH WHEGCFHCSR CGSSLVDKPF AAKEEQLLCT DCYSNEYSSK CQECKKTIMP GTRKMEYKGS SWHETCFTCQ RCQQPIGTKS FIPKENQNFC VPCYEKQYAL QCVQCKKPIT TGGVTYREQP WHKECFVCTA CKKQLSGQRF TARDEFPYCL TCFCDLYAKK CAGCTNPISG LGGTKYISFE ERQWHNDCFN CKKCSLSLVG RGFLTERDDI LCPDCGKDI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. Inhibits the transcriptional activity of FOXO1 and its apoptotic function by enhancing the interaction of FOXO1 with SIRT1 and FOXO1 deacetylation. Negatively regulates the calcineurin/NFAT signaling pathway in cardiomyocytes.
  • 基因功能参考文献：
    1. Fhl2 anticipates the emerging inflammation and specifically the development of psoriatic arthritis by impeding the Adam17-mediated release of TNF PMID: 28823868
    2. FHL2 facilitates ovarian granulosa cell tumor progression via controlling AKT1 transcription. PMID: 27415427
    3. Loss of FHL2 is associated with liver fibrosis. PMID: 28223370
    4. loss of Fhl2 leads to an altered immune response to myocardial ischemia, which results in smaller infarcts and better hemodynamic performance up to 21 days after myocardial ischemia reperfusion. PMID: 26921632
    5. also found that phosphorylation of ERK1/2 is markedly attenuated in FHL2-KO lung PMID: 26222912
    6. Overexpression of FHL2 alone had no effect on Erbin expression, but in the presence of MITF, Erbin expression was decreased. PMID: 26025865
    7. these data identify a previously unrecognized role for FHL2 in the pathogenesis of pulmonary granulomatous inflammation PMID: 26300344
    8. we conclude that FHL2 has both structural and functional protein-protein interactions with b-catenin in the podocyte nucleus and that FHL2 protein inhibition can mitigate Wnt/b-catenin-induced podocytopathy. PMID: 25855776
    9. FHL2 serves as a protective factor and that, rather than promoting the pathology, the upregulation of FHL2 in RA occurs in frame of a regenerative attempt. PMID: 25125695
    10. FHL2 regulates hematopoietic stem cell functions under transplantation-induced stress conditions. PMID: 25179730
    11. The LIM-only protein FHL2 reduces vascular lesion formation involving inhibition of proliferation and migration of smooth muscle cells. PMID: 24736599
    12. a database of genes that are regulated by FHL2 in liver PMID: 24453047
    13. FHL2-deficient mice developed a severe and long-lasting lung pathology due to enhanced expression of tenascin C and impaired activation of inflammation-resolving macrophages. PMID: 24260575
    14. Early Wnt/beta-catenin-dependent transcriptional activation mediated by FHL2 is important for the transition to and expansion of early cardiogenic mesodermal cells. PMID: 23341242
    15. Deficiency of FHL2 reduced susceptibility to diethylnitrosamine-induced hepatocarcinogenesis, correlating with the activator function of FHL2 in NF-kappaB signaling. PMID: 23775124
    16. Deficiency of FHL2 results in aggravation of murine liver fibrosis. PMID: 23311569
    17. Ex vivo analysis showed decreased basal and Wnt3a-induced Wnt5a and Wnt10b mRNA expression in FHL2-deficient bone marrow cells, further indicating that this defect may contribute to the reduced osteoblast function in FHL2 deficient mice PMID: 23201222
    18. The four and a half LIM-only protein 2 regulates liver homeostasis and contributes to carcinogenesis. PMID: 22796152
    19. ROCK2 is essential for the development of cardiac hypertrophy and that up-regulation of FHL2 may contribute to the antihypertrophic phenotype that is observed in cardiac-specific ROCK2-deficient mice. PMID: 23271052
    20. Deficiency of FHL2 impairs ischemia-induced neovascularization, and these suppressive effects may occur through a reduction in proangiogenic cell mobilization, migration, and vasculogenesis functions. PMID: 23413425
    21. FHL2 might play an important role in ameloblast and odontoblast differentiation, secretion of enamel and dentin matrix, mineralization of enamel, molar crown morphogenesis, as well as root formation. PMID: 22461197
    22. Observed is increased expression of isoproterenol-activated NFAT target genes in Fhl2-/- mice. PMID: 22851699
    23. FHL2-mediated induction of IL-6 was regulated by the activation of IL-6 promoter through stimulating NF-kappaB and p38 MAPK signaling pathway. PMID: 22633286
    24. role of FHL2 in intestinal tumorigenesis in which activation of the Wnt pathway by mutations in the adenomatous polyposis coli gene (Apc) or in beta-catenin constitutes the primary transforming event PMID: 20442768
    25. Collectively, these studies define a Fhl2-Foxk1 cascade that regulates the myogenic progenitor cell activity in adult skeletal muscle and enhances our understanding of muscle regeneration. PMID: 20013826
    26. A role for FHL2 is demonstrated in atherogenesis, the regulation of circular nitric oxide release, and expression of gap junctions within aortic endothelium. PMID: 20096293
    27. FHL2 and FHL3, respectively, are colocalized with alpha(7)beta(1) integrin receptor at the periphery of Z-discs, suggesting a role in mechanical stabilization of muscle cells PMID: 15117962
    28. data describe a novel differential regulation of Hand1 heterodimers versus homodimers by association of the cofactor FHL2 and provide insight into the potential for a tertiary level of control of Hand1 activity in the developing heart PMID: 15509787
    29. antagonizes induction of smooth muscle genes by competing with the coactivator MAL/MRTF-A for SRF binding PMID: 15610731
    30. Fhl2 increases the transcriptional activity of runt-related transcription factor 2 (Runx2) PMID: 16079911
    31. FHL2 plays an important role in osteoblast differentiation and bone formation. PMID: 16355270
    32. four-and-a-half LIM 2 has a role in bone mineral content and bone mineral density in femur and tibia bones PMID: 16927043
    33. MuRF3 has an essential role in maintaining cardiac integrity and function after acute myocardial infarction and turnover of FHL2 and gamma-filamin contributes to this cardioprotective function of MuRF3 PMID: 17360532
    34. Fhl2 is a lipid-triggered signaling molecule in mesenchymal cells regulating migration and contraction during cutaneous wound healing. PMID: 17420295
    35. Role of FHL2 in bundling of focal adhesion structures, in integrin-mediated ERK activation, and subsequently in proper allocation of matrix proteins on the cell surface. PMID: 18356303
    36. The decreased chemical-induced corneal angiogenesis found in the FHL2-null mice in this study indicated that FHL2 protein plays a role in inhibiting inflammatory angiogenesis. PMID: 18708619
    37. FHL2 is an important factor regulating collagen expression in the early phase of wound healing. PMID: 18950620
    38. deregulation of FHL2 contributes to the development of human myeloid disorders. PMID: 19369964

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Cytoplasm, myofibril, sarcomere, Z line.
  • 组织特异性：
    Highly expressed in heart but also detectable in brain and skeletal muscle.
  • 数据库链接：

    KEGG: mmu:14200

    STRING: 10090.ENSMUSP00000008280

    UniGene: Mm.6799