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  4. Recombinant Mouse Forkhead box protein O4 (Foxo4)

Recombinant Mouse Forkhead box protein O4 (Foxo4)

  • 货号：
    CSB-YP894383MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP894383MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP894383MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP894383MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP894383MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Foxo4; Afx; Afx1; Fkhr3; Mllt7Forkhead box protein O4; Afxh; Fork head domain transcription factor AFX1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-505
  • 氨基酸序列
    MDPENKKSAT GAAAILDLDP DFEPQSRPRS CTWPLPRPDL ATEPHEPSEV EPSLGQKVPT EGHSEPILLP SRLPEPAGGP QPGILGAVTG PRKGGSRRNA WGNQSYAELI SQAIESAPEK RLTLAQIYEW MVRTVPYFKD KGDSNSSAGW KNSIRHNLSL HSKFIKVHNE ATGKSSWWML NPDGGKGGKA PRRRAASMDS SSKLLRGRSK GPKKKPSVLP APPEGATPRS PLGHFAKWSS SPCPRNREEA DVWTTFRPRS SSNASTVSTR LSPMRPESEV LAEEEMPASA SSYAGGVPPT LSEDLELLDG LNLASPHSLL SRSGLSGFSL QHPGLAGPLH SYGASLFGPI DGSLSAGEGC FSSSQSLEAL LTSDTPPPPA DVLMTQVDPI LSQAPTLLLL GGMPSSSKLG TGVSLCPTPL EGPGPSNLVP NLSVMAPPPV MAGAPIPKVL GTPVLASPTE DSSHDRMPQD LDLDMYMENL ECDMDNIISD LMDGEGLDFN FEPDP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity. Represses smooth muscle cell differentiation by inhibiting the transcriptional coactivator activity of myocardin.
  • 基因功能参考文献：
    1. FOXO transcription factors play a salutary role in the protection against the diet-induced fatty liver disease. PMID: 28300161
    2. FoxOs exacerbate the loss of cancellous bone mass associated with type 1 diabetes. PMID: 27491024
    3. Foxo1, Foxo3a, and Foxo4 in chondrocytes regulate endochondral bone formation. PMID: 26232637
    4. The effect of Sirt1 stimulators on osteoclastogenesis was abrogated in cells lacking FoxO1, FoxO3, and FoxO4. PMID: 26287518
    5. FoxO4 is collectively required to maintain MODYrelated gene networks, which in turn are required to enable a gene expression program that permits proper substrate selection (glucose versus fatty acids) for mitochondrial oxidative phosphorylation. PMID: 25264246
    6. FoxO4 transcription factor is present during both oocyte and embryo in vivo maturation and FoxOs may regulate in vitro embryo development under stress conditions. PMID: 25929834
    7. FoxO4 activates Arg1 transcription in endothelial cells in response to MI, leading to downregulation of nitric oxide and upregulation of neutrophil infiltration to the infarct area. PMID: 26438688
    8. Foxo1, Foxo3, and Foxo4 transcriptional network regulates autophagy and the ubiquitin-proteasome system during muscle atrophy. PMID: 25858807
    9. Liver-specific ablation of FoxO1,FoxO3 and FoxO4 prevents the induction of glucose-6-phosphatase and the repression of glucokinase during fasting, thus increasing lipogenesis. PMID: 25307742
    10. FOXO1, FOXO3a and FOXO4, are indispensable for SIRT1-dependent cell survival against oxidative stress. PMID: 24040102
    11. FoxO1/3/4 transcription factors play important roles in hepatic glucose homeostasis. PMID: 24015318
    12. Mice lacking Foxo1, -3, and -4 in bipotential progenitors of osteoblast and adipocytes exhibit increased osteoblast number and high bone mass. PMID: 23867625
    13. Foxk1 promotes muscle progenitor cell proliferation by repressing Foxo4 transcriptional activity and inhibits myogenic differentiation by repressing Mef2 activity. PMID: 22956541
    14. Genetic ablation of the 3 genes encoding FoxO isoforms 1, 3a, and 4, in myeloid cells resulted in an expansion of the granulocyte/monocyte progenitor compartment and was associated with increased atherosclerotic lesion formation. PMID: 23420833
    15. FoxO4 inhibits atherosclerosis through bone marrow derived cells, possibly by inhibition of reactive oxygen species and inflammatory cytokines that promote monocyte recruitment and/or retention. PMID: 22005198
    16. In NIH3T3 cells, tetrahydrocurcumin induced nuclear accumulation of FOXO4 PMID: 22156377
    17. Data show that glomeruli isolated from diabetic db/db mice have increased acetylation of Foxo4, suppressed expression of Sirt1, and increased expression of Bcl2l11 compared to non-diabetic littermates. PMID: 21858169
    18. Foxo4 may be a useful target for suppression in the treatment of HBV-associated hepatocellular carcinoma cells. PMID: 21567078
    19. Data indicate that FoxOs work in concert to regulate multiple aspects of hepatic glucose metabolism. PMID: 20880840
    20. a conserved critical Ku70 role for FOXO function toward coordination of a survival program PMID: 20570964
    21. Using transcriptional assays, we observed that Fhl2, in a dose-dependent fashion, promotes Foxk1 transcriptional repression of Foxo4 activity. PMID: 20013826
    22. The forkhead transcription factor AFX activates apoptosis by induction of the BCL-6 transcriptional repressor. PMID: 11777915
    23. AFX (a Forkhead transcription factor = Foxo4) is expressed in the rodent ovary PMID: 11875118
    24. plays an important role in the regulation of GADD45 in response to oxidative stress and thereby contributes to G(2)-M checkpoint PMID: 12048180
    25. the MLL-AFX fusion protein requires the transcriptional effector domains of AFX to transform myeloid progenitors and interferes with forkhead protein function PMID: 12192052
    26. mechanism for oncogenic activation of MLL by forkhead family proteins designated FKHRL1 and AFX PMID: 12393557
    27. AGE-RAGE interaction contributes to podocyte apoptosis by activation of the FOXO4 transcription factor. PMID: 17667983
    28. FoxO factors mediate Myc-induced Arf expression and provide direct genetic evidence for their tumor-suppressive capacity. PMID: 17974917
    29. Mdm2 induces mono-ubiquitination of FOXO4 PMID: 18665269
    30. The effect of tumor necrosis factor on atrogin-1 expression via Foxo protein metabolism is reported. PMID: 18701653
    31. A link between AMPK, glucose clearance, foxo4 and the leptin axis, is suggested. PMID: 19410631
    32. FoxO4 inhibits NF-kappaB and protects mice against colonic injury and inflammation. PMID: 19560465
    33. FOXO proteins enforce DNA damage-induced cell cycle arrest in hematopoietic cells. PMID: 19671690

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 组织特异性：
    Strongly expressed in brown adipose tissue and weakly in white adipose tissue (at protein level). Expressed in skeletal muscle.
  • 数据库链接：

    KEGG: mmu:54601

    STRING: 10090.ENSMUSP00000059420

    UniGene: Mm.240299