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  4. Recombinant Mouse Forkhead box protein O1 (Foxo1)

Recombinant Mouse Forkhead box protein O1 (Foxo1)

  • 货号：
    CSB-YP865641MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP865641MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP865641MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP865641MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP865641MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Foxo1; Fkhr; Foxo1aForkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-652
  • 氨基酸序列
    MAEAPQVVET DPDFEPLPRQ RSCTWPLPRP EFNQSNSTTS SPAPSGGAAA NPDAAASLAS ASAVSTDFMS NLSLLEESED FARAPGCVAV AAAAAASRGL CGDFQGPEAG CVHPAPPQPP PTGPLSQPPP VPPSAAAAAG PLAGQPRKTS SSRRNAWGNL SYADLITKAI ESSAEKRLTL SQIYEWMVKS VPYFKDKGDS NSSAGWKNSI RHNLSLHSKF IRVQNEGTGK SSWWMLNPEG GKSGKSPRRR AASMDNNSKF AKSRGRAAKK KASLQSGQEG PGDSPGSQFS KWPASPGSHS NDDFDNWSTF RPRTSSNAST ISGRLSPIMT EQDDLGDGDV HSLVYPPSAA KMASTLPSLS EISNPENMEN LLDNLNLLSS PTSLTVSTQS SPGSMMQQTP CYSFAPPNTS LNSPSPNYSK YTYGQSSMSP LPQMPMQTLQ DSKSSYGGLN QYNCAPGLLK ELLTSDSPPH NDIMSPVDPG VAQPNSRVLG QNVMMGPNSV MPAYGSQASH NKMMNPSSHT HPGHAQQTAS VNGRTLPHVV NTMPHTSAMN RLTPVKTPLQ VPLSHPMQMS ALGSYSSVSS CNGYGRMGVL HQEKLPSDLD GMFIERLDCD MESIIRNDLM DGDTLDFNFD NVLPNQSFPH SVKTTTHSWV SG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'-TT[G/A]TTTAC-3'. Activity suppressed by insulin. Main regulator of redox balance and osteoblast numbers and controls bone mass. Orchestrates the endocrine function of the skeleton in regulating glucose metabolism. Also acts as a key regulator of chondrogenic commitment of skeletal progenitor cells in response to lipid availability: when lipids levels are low, translocates to the nucleus and promotes expression of SOX9, which induces chondrogenic commitment and suppresses fatty acid oxidation. Acts synergistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity. Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A and CEBPA to activate the expression of genes such as IGFBP1, G6PC1 and PCK1. Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and STK4/MST1. Promotes neural cell death. Mediates insulin action on adipose tissue. Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake. Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells. Required for the autophagic cell death induction in response to starvation or oxidative stress in a transcription-independent manner. Mediates the function of MLIP in cardiomyocytes hypertrophy and cardiac remodeling. Regulates endothelial cell (EC) viability and apoptosis in a PPIA/CYPA-dependent manner via transcription of CCL2 and BCL2L11 which are involved in EC chemotaxis and apoptosis.
  • 基因功能参考文献：
    1. Furthermore, chromatin immunoprecipitation (ChIP) followed by luciferase assays revealed direct binding of Foxo1 to both the Il9 and Irf4 promoters and induces their transactivation. PMID: 29339772
    2. Japanese encephalitis virus (JEV) induced cell apoptosis by inhibiting STAT3-Foxo-Bcl-6/p21 pathway, which provides a novel insight into JEV-caused encephalitis. PMID: 29885805
    3. data reveal the pervasive role of forkhead box O1(FoxO1) in mediating the effects of insulin on not only glucose metabolism but also other hormonal signaling pathways and even some aspects of lipid metabolism PMID: 29300910
    4. FOXO1 deletion in epithelium led to impaired healing that included decreased formation of new connective tissue. PMID: 28220813
    5. Distinct levels of phosphorylated FoxO1 were observed. PMID: 29701296
    6. the results of the present study suggest that moderate overexpression of SIRT1 (~3fold of normal level) may directly or indirectly inhibit apoptosis of OBs via the FOXO1 and betacatenin signaling pathway. PMID: 29512706
    7. insulin-activated SREBP1c downregulates gluconeogenesis through CRY1-mediated FOXO1 degradation. PMID: 27412556
    8. Our study demonstrated that Arachidonic acid (AA) inhibits macrophage viability by inducing S phase cell cycle arrest. The JNK signaling pathway and the downstream FoxO transcription factors are involved in AA-induced RAW264.7 cell cycle arrest. PMID: 29426338
    9. the transcription of Rho-associated coiled-coil containing protein kinase 1 (ROCK1), which phosphorylates Drp1 at Ser616, was shown by luciferase assay to be directly regulated by FOXO1. These findings suggested that FOXO1 is critical to preserve mitochondrial quantity and function in ECs, and FOXO1 may serve as a therapeutic target for microvascular complications of diabetes. PMID: 29654945
    10. Chimeric antigen receptor T cells releasing IL-18 convert to T-Bet(high) FoxO1(low) effectors that exhibit augmented activity against advanced solid tumors. PMID: 29241547
    11. FOXO1 is dispensable for naive T cell expression of TCF7, it is essential for the expression of TCF7 in a small subset of T cells within days following primary infection. PMID: 28973925
    12. Foxo1 expression compromised embryonic stem cell self-renewal PMID: 28622295
    13. we identified that Sirtuin 1 (SIRT1), a deacetylase that suppresses FoxO1 acetylation in granulosa cell (GCs), was downregulated by miR-181a and reversed the promoting effects of H2O2 and miR-181a on FoxO1 acetylation and GC apoptosis. PMID: 28981116
    14. Study identified the roles of Foxo1 as a positive regulator and Foxp1 as a negative regulator of TH9 cell differentiation and antitumor activity. PMID: 29018172
    15. miR-150-mediated suppression of Foxo1 regulates the balance between effector and memory cell differentiation PMID: 28903041
    16. Inhibition of FOXO1 enhanced angiogenesis in human bio-engineered capillaries, and resulted in microvascular regeneration and improved function in mouse models of injury-repair. PMID: 28711779
    17. s found that the repress effect of alphaMSH in adipocytes apoptosis is acting through Foxo1/mTORC2 pathway. These findings indicate that, alphaMSH has a strong inhibitory effect on ROS-induced adipocyte apoptosis and underlying mechanism is interacting with key factors in mTOR signal pathway. PMID: 28388573
    18. Sirt3 activation is essential to improve autophagy flux by reducing the acetylation modification on FoxO1, which in turn alleviates myocardial hypertrophy. PMID: 27880725
    19. The data reveal a novel mechanism in which the elevated miR-425 in IBD mediates pathogenic Th17 cell generation through down-regulation of Foxo1. PMID: 29331376
    20. we found that in db/db VSMC, the occupancy in promoter regions of inflammatory genes by FOXO1 was reduced.miR-135a increased the inflammatory responses of VSMC involved in complications of vascular diseases by downregulating the expression of FOXO1. PMID: 29332916
    21. FoxO1 is a key mediator involved in glucose homeostasis and indicate that 1,25(OH)2D3 improves glucose metabolism and bone development PMID: 29042442
    22. Data show that the mitochondrial enriched GCN5-like 1 protein (GCN5L1) controls hepatic glucose production by regulating FoxO1 protein levels. PMID: 28900165
    23. Setdb1 regulates PTEN/AKT/FOXO1 pathway to inhibit Spermatogonial stem cells apoptosis. PMID: 28890329
    24. These findings indicate that IGF-II reduces PGC-1alpha expression in skeletal muscle cells through a mechanism involving PI3K-Akt-FoxO1 but not p38 MAPK or Erk1/2 MAPK pathways. PMID: 28374141
    25. a critical role for FOXO transcription factors in mediating these proliferative versus apoptotic fates PMID: 29103953
    26. L. donovani triggered AKT activation to regulate GSK-3beta/beta-catenin/FOXO-1 axis. PMID: 27662364
    27. The data suggest autocrine nitric oxide/atrial natriuretic peptide-induced activation of protein kinase G type Ialpha/p-AKT/p-FOXO1 promotes survival and proliferation in pancreatic beta-cells. PMID: 28631500
    28. Myocardial ischemia is associated with downregulation and posttranslational modification of cardiac FoxO1. In a mouse model of postischemic heart failure, posttranslational modulation of FoxO1 alters heart function involving collagen and protein metabolism. PMID: 27105158
    29. Cell fate and metabolic state are linked by transcriptional regulators, such as IRF4 and FoxO1, with dual roles in lineage and metabolic choice. Instructing some cells to utilize nutrients for anabolism and differentiation while other cells catabolically self-digest and self-renew may enable growth and repair in metazoa. PMID: 28009285
    30. results suggest that FoxO1OB might be involved in the regulation of 1,25(OH)2D3 on glucose homeostasis and bone formation, and that FoxO1OB might act as a key modulator of the capacity of the skeleton regulating metabolic homeostasis. Our study also provides a new idea that a combination of systemic 1,25(OH)2D3 and local FoxO1 inhibitor may be a new approach to enhance implant osseointegration. PMID: 29080745
    31. data reveal apelin as a novel regulator of FoxO1 in cardiac cells and provide evidence for the potential of apelin-13 in prevention of apoptosis and mitochondrial damage in conditions combining I/R injury and obesity. PMID: 27005319
    32. Besides, both in vivo and in votro studies suggested that K145 stimulated insulin dependent Akt phosphorylation and subsequently activates FoxO1 phosphorylation therefore inhibited gluconeogenetic genes expression including PEPCK and G6pase. Our study figures out a potential extent increase the value of developing K145 as therapeutic candidate for diabetes. PMID: 28911865
    33. this study shows that Foxo1 is a positive regulatory factor for the proliferation and activity of Treg cells PMID: 28283017
    34. Data show that Fos-Related Antigen-2 (Fra-2) is a key upstream regulator of forkhead box O1 (Foxo1) and interferon regulatory factor 4 (Irf4) expression and influences proliferation and differentiation of B cells at multiple stages. PMID: 28566276
    35. Link between the antioxidative activity of FoxO1 with PINK1/Parkin-induced mitophagy, indicating a novel role of FoxO1 in diabetic nephropathy. PMID: 28505239
    36. the overlap of IR and IGF1R signaling is critical to the regulation of muscle protein turnover, and this regulation depends on suppression of FoxO-regulated, autophagy-mediated protein degradation PMID: 27525440
    37. These data strongly suggest that intestinal apoC-III is not a FoxO1 target and support the idea that apoC-III is not regulated coordinately with hepatic apoC-III, and establishes another key aspect of apoC-III that is unique in the intestine from the liver. PMID: 28739253
    38. this study shows that miR-183C drives Th17 pathogenicity in experimental autoimmune encephalomyelitis via inhibition of Foxo1 PMID: 27332731
    39. The findings of this study suggest that Foxo-regulated pathways are downstream of Isl1 in the survival and/or differentiation of direct pathway striatal projection neurons. PMID: 28213137
    40. Endothelial FoxO proteins promote insulin resistance in HF diet, which may in part result from FoxO proteins establishing an antiangiogenic and proinflammatory microenvironment within skeletal muscle. These findings provide mechanistic insight into the development of microvascular dysfunction in the progression of type 2 diabetes.- PMID: 27235148
    41. ablation of Foxo1 after Germinal center (GC) development led to the loss of the dark zone GC B cells and disruption of the GC architecture. PMID: 28351982
    42. Suppression of Sirt1 or Foxo-1 expression significantly impaired exendin-4-induced adiponectin expression. Consistently, exendin-4 up-regulated Sirt1 and Foxo-1 expression in vivo. Our work is the first study demonstrating the role of Sirt1/Foxo-1 in regulating the regulatory function of a GLP-1 receptor agonist in adiponectin expression both in vitro and in vivo. PMID: 28122026
    43. Increased Forkhead box protein 1 (FoxO1) acetylation and nuclear retention was followed by progressive FoxO1 loss in beta cells that marked the onset of diabetes. PMID: 28057752
    44. Results show that microRNA miR-181c attenuated nitration stress through regulating forkhead box O1 (FoxO1) expression and affecting endothelial cell function, suggesting a target for the development of preventive or therapeutic agents against diabetes mellitus (DM). PMID: 28223216
    45. Lysophosphatidic acid/PKD-1 signaling leads to nuclear accumulation of histone deacetylase 7, where it interacts with forkhead box protein O1 to suppress endothelial CD36 transcription and mediates silencing of antiangiogenic switch, resulting in proangiogenic and proarteriogenic reprogramming. PMID: 27013613
    46. A role of FOXO1 in the regulatory T cell differentiation process.TGF-beta induces the phosphorylation of FOXO1 in CD4+ T cells. PMID: 27697523
    47. Fucosterol inhibited adipogenesis of 3T3-L1 preadipocytes through modulation of FoxO signalling pathway. PMID: 28134973
    48. We verified a positive correlation between the level of mir- 182 and symptom severity of experimental autoimmune encephalomyelitis (EAE) in mice and provide evidence that mir-182 inhibited Treg cells specialization through Foxo1 dependent pathway, during the pathogenesis of EAE PMID: 27664932
    49. These findings suggest that specific overexpression of renal FoxO1 decreases podocyte apoptosis, which may be explained in part by its regulation of PINK1, and that targeting FoxO1 may represent a novel therapeutic approach for diabetic nephropathy. PMID: 27475499
    50. However, Prep1 doesn't affect gluconeogenesis in the absence of insulin. Also, we observed that nuclear content of gluconeogenic transcription factor FOXO1 was greatly reduced in Prep1 knockout hepatocytes. These findings suggest that Prep1 may control hepatic insulin sensitivity by targeting FOXO1 nuclear stability. PMID: 27815072

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 组织特异性：
    Expressed in liver, white and brown adipose tissues (at protein level).
  • 数据库链接：

    KEGG: mmu:56458

    STRING: 10090.ENSMUSP00000055308

    UniGene: Mm.29891