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  4. Recombinant Mouse Follitropin subunit beta (Fshb)

Recombinant Mouse Follitropin subunit beta (Fshb)

  • 货号：
    CSB-YP717119MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP717119MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP717119MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP717119MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP717119MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    FshbFollitropin subunit beta; Follicle-stimulating hormone beta subunit; FSH-B; FSH-beta; Follitropin beta chain
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    21-130
  • 氨基酸序列
    SCELTNITIS VEKEECRFCI SINTTWCAGY CYTRDLVYKD PARPNTQKVC TFKELVYETV RLPGCARHSD SLYTYPVATE CHCGKCDSDS TDCTVRGLGP SYCSFSEMKE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Together with the alpha chain CGA constitutes follitropin, the follicle-stimulating hormone, and provides its biological specificity to the hormone heterodimer. Binds FSHR, a G protein-coupled receptor, on target cells to activate downstream signaling pathways. Follitropin is involved in follicle development and spermatogenesis in reproductive organs.
  • 基因功能参考文献：
    1. FSH induces autophagy via upregulation of HIF-1A in mouse granulosa cells. PMID: 28817115
    2. the in vivo bioactivities of FSH glycoforms in Fshb null mice using a pharmacological rescue approach, was investigated. PMID: 27561202
    3. These mice showed an increase in FSH levels, but they remained in a prepubertal state. Together with previous findings, our data indicate that leptin-selective action in gonadotropes serves a role in adult reproductive physiology but is not sufficient to allow pubertal maturation in mice. PMID: 27101301
    4. GnRH stimulated the secretion of the VGF-derived peptide NERP1. NERP1 caused a concentration-dependent decrease in Fshb gene induction. PMID: 27466366
    5. Data show that estradiol or bisphenol A decreased expression of luteinizing hormone beta (Lhb), follicle stimulating hormone beta (Fshb), and intracellular adhesion molecule-5 (Icam5) in females but only decreased expression of Icam5 in males. PMID: 26789235
    6. GnRH stimulation of Fshb expression is dependent on miR-132/212 and involves a SIRT1-FOXO1 pathway. PMID: 25635942
    7. results suggest that FOXO1 binding to the proximal Fshb promoter as well as FOXO1 interaction with SMAD3/4 proteins may result in decreased activin induction of Fshb in gonadotropes PMID: 25423188
    8. SCGB3A2 regulates FSH/LH production in the anterior pituitary lobe. PMID: 24514953
    9. Data suggest that the BMP2-like Activin A/BMP2 chimera AB215 regulates follicle stimulating hormone beta (FSHbeta) induction in LbetaT2 gonadotroph cells due to its ability to block activin A signaling. PMID: 25100748
    10. Two regions of the proximal Fshb promoter (-50/-41 and -30/-21) appear to be necessary for FOXO1 suppression of GnRH-induced Fshb transcription. PMID: 24065703
    11. Data suggest BMP2 (bone morphogenetic protein 2) stimulates SMAD2/3 signaling in gonadotrophs via Bmpr1a (bone morphogenetic protein receptor type 1A); such signaling via SMAD3 appears to be necessary for up-regulation of Fshb transcription. PMID: 24601881
    12. The activin A signals via SMAD proteins, but not TAK1 or p38, to regulate murine and ovine Fshb transcription in gonadotrope-like cells. PMID: 22549017
    13. The insulin/IGF signaling pathway is required for FSH-mediated Sertoli cell proliferation. PMID: 23518924
    14. FOXL2 is required for FSH synthesis in vivo. PMID: 23340250
    15. Data indicate that free fatty acids up-regulated luteinizing hormone beta (Lhb) mRNA expression acutely but suppressed follicle stimulating hormone beta (Fshb) mRNA expression. PMID: 23525221
    16. Blocking antibody to the beta-subunit of FSH prevents bone loss by inhibiting bone resorption and stimulating bone synthesis. PMID: 22908268
    17. Protein-protein interactions among FOXL2, SMAD3, and progesterone receptor (PR) recruited to the Fshb promoter play a key role in facilitating Fshb transcription before the secondary FSH surge in rodents. PMID: 22294749
    18. the pituitary gonadotrope contains an ultra-short, activin-responsive feedback loop utilizing two different isoforms of Smad3, one which acts as an agonist and another that acts as an intracrine antagonist to regulate FSH-beta production. PMID: 21664424
    19. These data indicate that, in addition to its role in the ovary, FoxL2 function in the pituitary is required for normal expression of FSH. PMID: 21700720
    20. SMADs and FOXL2 synergistically regulate murine follicle stimulating hormone beta transcription via a conserved proximal promoter element PMID: 21622537
    21. The LIM-homeodomain transcription factor Isl-1 functions to increase FSHbeta/LHbeta gene transcription, and mediates the effects of leptin on gonadotropin synthesis. PMID: 20702576
    22. It was shown that SMAD4 has a necessary role in activin A induction of the murine FSHb gene in immortalized gonadotropes. PMID: 20371653
    23. FoxL2 plays a key role in activin induction of the FSHbeta gene, by binding to sites conserved across multiple species. PMID: 20233786
    24. FSH promotes primordial follicle formation in mice by stimulating local activin signaling pathways and the expression of oocyte-specific transcription factors. PMID: 19641178
    25. suggest that inducible cAMP early repressor production antagonizes the stimulatory action of cyclic AMP response element binding protein to attenuate FSH beta transcription at high gonadotropin-releasing hormone pulse frequencies. PMID: 20008557
    26. GnRH regulation of the FSHbeta gene occurs through induction of multiple Fos and Jun isoforms. PMID: 14570911
    27. Data suggest that activin receptor II signaling is critical only for follicle-stimulating hormone homeostasis and not for gonadotrophin-releasing hormone (GnRH)biosynthesis or induction of pituitary GnRH receptor expression. PMID: 14654247
    28. activins use both SMAD2- and SMAD3-dependent mechanisms to stimulate FSHbeta transcription in mouse gonadotrope cells. PMID: 14701940
    29. androgens, glucocorticoids, and progestins act via their receptors to directly activate FSHbeta gene expression in the pituitary gonadotrope PMID: 16675544
    30. Hypogonadal bone loss is caused, at least in part, by enhanced FSH secretion, which in turn increases TNFalpha production to expand the number of bone marrow osteoclast precursors. PMID: 17003115
    31. Synergistic interaction between activin and glucocorticoids within gonadotrope cell. This synergy can occur directly at level of mouse FSHbeta promoter. PMID: 17082263
    32. Results show that GnRH overcomes histone deacetylase (HDAC)-mediated repression of the FSH and LH beta-subunit genes in immature gonadotropes. PMID: 17371839
    33. Steroid receptors physically interact with Smads and are sufficient for the synergism to occur on the FSHbeta promoter. PMID: 18079204
    34. The knockdown of PRPF19 or EIF4A3 decreased splicing of Lhb, or of both beta subunit transcripts, respectively. PMID: 18480465
    35. Bone morphogenetic protein 2 signals via BMPR1A to regulate murine follicle-stimulating hormone beta subunit transcription. PMID: 19211807
    36. study concludes that the rapid effect of GNRH1 on Fshb expression in LbetaT2 cells is mediated by PGR phosphorylation and loading at the PGR response element within the Fshb promoter together with NCOA3 PMID: 19357364

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Glycoprotein hormones subunit beta family
  • 数据库链接：

    KEGG: mmu:14308

    STRING: 10090.ENSMUSP00000028533

    UniGene: Mm.249525