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  4. Recombinant Mouse F-box/WD repeat-containing protein 7 (Fbxw7)

Recombinant Mouse F-box/WD repeat-containing protein 7 (Fbxw7)

  • 货号：
    CSB-YP851809MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP851809MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP851809MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP851809MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP851809MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Fbxw7; Fbw7; Fbwd6; Fbxw6F-box/WD repeat-containing protein 7; F-box and WD-40 domain-containing protein 7; F-box protein FBW7; F-box protein Fbxw6; F-box-WD40 repeat protein 6; SEL-10
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-629
  • 氨基酸序列
    MRVCVPSSVL VLSCVCWCWG VLLPVPLPNL PFLACLSMST LESVTYLPEK GLYCQRLPSS RTHGGTESLK GKNTENMGFY GTLKMIFYKM KRKLDHGSEV RSFSLGKKPC KVSDYTSTTG LVPCSATPTT FGDLRAANGQ GQQRRRITSV QPPTGLQEWL KMFQSWSGPE KLLALDELID SCEPTQVKHM MQVIEPQFQR DFISLLPKEL ALYVLSFLEP KDLLQAAQTC RYWRILAEDN LLWREKCKEE GIDEPLHIKR RKIIKPGFIH SPWKSAYIRQ HRIDTNWRRG ELKSPKVLKG HDDHVITCLQ FCGNRIVSGS DDNTLKVWSA VTGKCLRTLV GHTGGVWSSQ MRDNIIISGS TDRTLKVWNA ETGECIHTLY GHTSTVRCMH LHEKRVVSGS RDATLRVWDI ETGQCLHVLM GHVAAVRCVQ YDGRRVVSGA YDFMVKVWDP ETETCLHTLQ GHTNRVYSLQ FDGIHVVSGS LDTSIRVWDV ETGNCIHTLT GHQSLTSGME LKDNILVSGN ADSTVKIWDI KTGQCLQTLQ GPSKHQSAVT CLQFNKNFVI TSSDDGTVKL WDLKTGEFIR NLVTLESGGS GGVVWRIRAS NTKLVCAVGS RNGTEETKLL VLDFDVDMK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter bring them to the SCF complex for ubiquitination. Mediates ubiquitination and subsequent degradation of CCNE1 and MYC. Identified substrates include cyclin-E (CCNE1 or CCNE2), DISC1, JUN, MYC, NOTCH1 released notch intracellular domain (NICD), NOTCH2, MCL1 and probably PSEN1. Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation. SCF(FBXW7) complex mediates the ubiquitination and subsequent degradation of NFE2L1. Involved in bone homeostasis and negative regulation of osteoclast differentiation. Regulates the amplitude of the cyclic expression of hepatic core clock genes and genes involved in lipid and glucose metabolism via ubiquitination and proteasomal degradation of their transcriptional repressor NR1D1; CDK1-dependent phosphorylation of NR1D1 is necessary for SCF(FBXW7)-mediated ubiquitination. Also able to promote 'Lys-63'-linked ubiquitination in response to DNA damage. The SCF(FBXW7) complex facilitates double-strand break repair following phosphorylation by ATM: phosphorylation promotes localization to sites of double-strand breaks and 'Lys-63'-linked ubiquitination of phosphorylated XRCC4, enhancing DNA non-homologous end joining.
  • 基因功能参考文献：
    1. Loss of Fbxw7 in the presence of BrafV600E mutation is consequential and sufficient to drive melanoma development. PMID: 28581198
    2. A novel mouse line carrying a conditional knockin allele of a cancer-specific FBXW7 mutation was established for carcinogenesis study. PMID: 29386660
    3. results thus suggest that Fbxw7 controls the transcription of MyRF target genes in various tissues through regulation of MyRF protein stability in a manner dependent on MyRF phosphorylation by GSK-3. PMID: 29472293
    4. FBXW7 is critical for RIG-I stabilization during antiviral responses. PMID: 28287082
    5. FBXW7 is markedly downregulated in the liver of obese mice. Mechanistically, FBXW7 directly binds to hepatokine fetuin-A to induce its ubiquitination and subsequent proteasomal degradation, comprising an important mechanism maintaining glucose homeostasis. PMID: 29475832
    6. the regulatory crosstalk between KLF5, miR-29a, and Fbw7/CDC4 cooperatively promotes atherosclerotic development PMID: 29074464
    7. found that Fbw7 loss caused activation of NF-kappaB signaling. Thus, FBW7 plays a protective role in acute intestinal inflammation by modulating the inflammatory response of NF-kappaB pathway. PMID: 29550488
    8. EglN2 might act as an FBW7 ubiquitin ligase substrate contributing to the progression of triple negative breast cancer. PMID: 28036276
    9. These findings highlight the molecular basis of Hajdu-Cheney syndrome (HCS) pathogenesis and provide clinical insights into potential targeted therapeutic strategies for skeletal disorders associated with the aberrant FBW7/NOTCH2 pathway as observed in patients with HCS. PMID: 29149593
    10. The findings reveal a PLK1-Fbw7-Myc signaling circuit that underlies tumorigenesis and validate PLK1 inhibitors, alone or with Bcl2 antagonists, as potential effective therapeutics for MYC-overexpressing cancers. PMID: 27773673
    11. Fbxw7 suppresses KrasG12D-induced pancreatic tumorigenesis via a Yap-dependent mechanism. PMID: 27764699
    12. Myoblast differentiation potential and muscle regeneration can be regulated by Fbxw7beta. PMID: 27594513
    13. Study identifies a REV-ERBalpha post-translational regulatory circuit in which cyclin-dependent kinase 1 (CDK1) phosphorylation of REV-ERBalpha is recognized by the F-box protein, FBXW7alpha, to direct REV-ERBalpha degradation via the proteasome. Disruption of this CDK1-FBXW7-mediated REV-ERBalpha degradation pathway in mouse liver alters circadian rhythmicity, in particular amplitude, and whole-body lipid/glucose home... PMID: 27238018
    14. Gene expression profiling reveals transcriptional regulation by Fbxw7/mTOR pathway in radiation-induced mouse thymic lymphomas. PMID: 26575021
    15. Prion infection induced the expression of FBXW7 in brain. PMID: 25579381
    16. FBXW7 facilitates nonhomologous end-joining via K63-linked polyubiquitylation of XRCC4 in tumor cells. PMID: 26774286
    17. data demonstrate that Fbw7alpha negatively regulates osteogenesis by targeting Runx2 for ubiquitin-mediated degradation in a GSK3beta-dependent manner PMID: 26542806
    18. In mice, an unusually direct antagonism between an E3 ligase and a deubiquitinase, Fbw7 and Usp28, modulate intestinal homeostasis and cancer. PMID: 25716680
    19. These data show that cell cycle-dependent mechanisms can control ciliary length through a CDK5-FBW7-NDE1 pathway. PMID: 26206584
    20. Dual regulation of Fbw7 activity by Usp28 is a safeguard mechanism for maintaining physiological levels of proto-oncogenic Fbw7 substrates, which is equivalently disrupted by loss or overexpression of Usp28. PMID: 25437563
    21. Ubiquitin-dependent degradation of GATA 2 is promoted by Fbw7, is cyclin B-CDK1-mediated Thr176 phosphorylation-dependent, and influences hematopoietic cell differentiation. PMID: 25670854
    22. Fbw7 is a master regulator of cell fate decisions in the pancreas PMID: 25105579
    23. FBXW7 modulates cellular stress response and metastatic potential through HSF1 post-translational modification. PMID: 25720964
    24. these results suggest that FBXW7 antagonizes cancer development in not only a cell-autonomous manner, but also a non-cell-autonomous manner PMID: 25555218
    25. demonstrate the importance of Fbw7-dependent cyclin E control to the hematopoietic system and highlight chromosome instability as a characteristic feature of dysfunction and malignancy induced by deregulated cyclin E PMID: 24958101
    26. p50 upregulated c-Myc protein expression mainly through inhibiting its degradation. p50 exhibited this novel property by suppression of FBW7 expression. PMID: 24457827
    27. Data propose that control of GATA3 levels by Fbw7 contributes to the fine-tuning of T-cell development. PMID: 24820417
    28. FBXW7 regulates spermatogonial stem cell self-renewal in a negative manner by degradation of MYC PMID: 24879440
    29. Heterozygous Fbxw7 propellor tip (R482Q) mutations promote intestinal tumors in mice. Heterozygous null Fbxw7 mutations also promote tumours, but the effect is weaker than R482Q. Findings explain the FBXW7 mutation spectrum found in human cancers. PMID: 23676439
    30. Fbw7 together with GSK3beta negatively regulates G-CSFR expression and its downstream signaling. PMID: 23820376
    31. the downstream Notch signalling effector HES5 directly represses transcription of the E3 ligase Fbw7beta. PMID: 23776410
    32. Loss of parkin function through biallelic mutation of PARK2 may lead to death of dopaminergic neurons through unregulated SCF(Fbw7beta)-mediated ubiquitylation-dependent proteolysis of Mcl-1. PMID: 23858059
    33. inhibition of mTOR signaling pathway suppresses the contribution of Fbxw7 loss toward tumor development. PMID: 23454868
    34. Fbxw7 controls proliferation and differentiation of keratinocytes, and exerts both inhibitory and stimulatory actions in skin carcinogenesis by counteracting the proliferation-promoting effect of c-Myc and tumor-suppressive effect of Notch. PMID: 22665065
    35. findings show that Fbxw7 plays a pivotal role in maintenance of quiescence in nondividing leukemia-initiating cells of chronic myeloid leukemia by reducing the level of c-Myc PMID: 23518349
    36. We demonstrate here the essential function of the Fbw7 E3 ligase for the initiation and the progression of chronic myelogenous leukemia. PMID: 23518350
    37. a number of miR-25 candidate gene targets PMID: 22912667
    38. SCF(Fbw7) modulates the NFkB signaling pathway by targeting NFkB2 for ubiquitination and destruction. PMID: 22708077
    39. Fbxw7 is a potent positive regulator of angiogenesis that limits the activity of Notch in the endothelium of the growing vasculature. PMID: 22848434
    40. Depletion of Fbxw7 resulted in promotion of induced pluripotent stem cell generation. PMID: 22897623
    41. Fbxw7 was decreased in NAFLD and negatively correlated with SREBP-1, indicating that the Fbxw7-SREBP-1 axis may play a key pathological role in the development of NAFLD. PMID: 22710480
    42. identify Fbxw7 as a p53-dependent tumor susceptibility gene. PMID: 22348067
    43. The F-box protein Fbw7 is required for cerebellar development. PMID: 21827743
    44. Fbxw7 as a key regulator of the maintenance and differentiation of neural stem cells in the brain. PMID: 21349854
    45. Loss of Fbxw7 is associated with oxidative stress. PMID: 21205095
    46. FBXW7 influences murine intestinal homeostasis and cancer, targeting Notch, Jun, and DEK for degradation. PMID: 21282377
    47. the E3 ubiquitin ligase SCF(FBW7) governs cellular apoptosis by targeting MCL1, a pro-survival BCL2 family member, for ubiquitylation and destruction in a manner that depends on phosphorylation by glycogen synthase kinase 3 PMID: 21368833
    48. Hepatic ablation of Fbxw7 resulted in hepatomegaly and steatohepatitis, with massive deposition of triglyceride, a phenotype similar to that observed in humans with nonalcoholic steatohepatitis. PMID: 21123947
    49. C/EBPdelta directly inhibits expression of the tumour suppressor F-box and WD repeat-domain containing 7 gene (FBXW7, FBW7, AGO, Cdc4), encoding an F-box protein that promotes degradation of the mammalian target of rapamycin (mTOR). PMID: 21076392
    50. miR-223 expression is responsive to acute alterations in cyclin E regulation by the Fbw7 pathway. PMID: 20826802

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  • 亚细胞定位：
    Nucleus, nucleoplasm. Chromosome.
  • 组织特异性：
    Widely expressed with highest levels in brain, heart and testis.
  • 数据库链接：

    KEGG: mmu:50754

    STRING: 10090.ENSMUSP00000103305

    UniGene: Mm.196475