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  4. Recombinant Mouse Eukaryotic elongation factor 2 kinase (Eef2k)

Recombinant Mouse Eukaryotic elongation factor 2 kinase (Eef2k)

  • 中文名称：
    小鼠Eef2k重组蛋白
  • 货号：
    CSB-YP007435MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Eef2k重组蛋白
  • 货号：
    CSB-EP007435MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Eef2k重组蛋白
  • 货号：
    CSB-EP007435MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Eef2k重组蛋白
  • 货号：
    CSB-BP007435MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Eef2k重组蛋白
  • 货号：
    CSB-MP007435MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Eef2k
  • Uniprot No.：
  • 别名：
    Eef2kEukaryotic elongation factor 2 kinase; eEF-2 kinase; eEF-2K; EC 2.7.11.20; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-724
  • 氨基酸序列
    MADEDLIFCL EGVDGGRCSR AGHNADSDTD SDDDEGYFIC PITDDHMSNQ NVSSKVQSYY SNLTKTECGS TGSPASSFHF KEAWKHAIEK AKHMPDPWAE FHLEDIATEH ATRHRYNAVT GEWLKDEVLI KMASQPFGRG AMRECFRTKK LSNFLHAQQW KGASNYVAKR YIEPVDRSVY FEDVQLQMEA KLWGEDYNRH KPPKQVDIMQ MCIIELKDRP GQPLFHLEHY IEGKYIKYNS NSGFVRDDNI RLTPQAFSHF TFERSGHQLI VVDIQGVGDL YTDPQIHTEK GTDFGDGNLG VRGMALFFYS HACNRICQSM GLTPFDLSPR EQDAVNQSTR LLQSAKTILR GTEEKCGSPR IRTLSSSRPP LLLRLSENSG DENMSDVTFD SLPSSPSSAT PHSQKLDHLH WPVFGDLDNM GPRDHDRMDN HRDSENSGDS GYPSEKRSDL DDPEPREHGH SNGNRRHESD EDSLGSSGRV CVETWNLLNP SRLHLPRPSA VALEVQRLNA LDLGRKIGKS VLGKVHLAMV RYHEGGRFCE KDEEWDRESA IFHLEHAADL GELEAIVGLG LMYSQLPHHI LADVSLKETE ENKTKGFDYL LKAAEAGDRH SMILVARAFD TGLNLSPDRC QDWSEALHWY NTALETTDCD EGGEYDGIQD EPQYALLARE AEMLLTGGFG LDKNPQRSGD LYTQAAEAAM EAMKGRLANQ YYEKAEEAWA QMEE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.
  • 基因功能参考文献：
    1. This study provides new insights into the control of eEF2K by AMPK. PMID: 28502587
    2. eEF2K activity is increased in postmortem Alzheimer's disease (AD) patient cortex and hippocampus, and in the hippocampus of aged transgenic AD mice. eEF2K inhibition using pharmacological or genetic approaches prevented the toxic effects of Abeta42 oligomers on neuronal viability and dendrite formation in vitro. Findings highlight the potential utility of eEF2K inhibition to reduce Abeta-mediated oxidative stress in AD. PMID: 27752775
    3. results suggest that protective versus pro-apoptotic roles of eEF2K depend on the type of cells: eEF2K is protective in highly proliferative cells, such as small intestinal stem cells and cancer cells, which are more susceptible to mitotic catastrophe. PMID: 27466362
    4. Using an eEF2K gain-of-function design in vitro as well as eEF2K-KO mice, we show that eEF2K activity regulates the excitation/inhibition balance by downregulating vesicle release at inhibitory synapses and tonic inhibition. This translates into a reduced susceptibility of eEF2K-KO mice for seizures without major behavioral alterations with the exception of impaired contextual and trace fear memory PMID: 27005990
    5. eEF2K protects cancer cells against nutrient starvation by inhibiting protein synthesis rather than by activating autophagy. PMID: 26795954
    6. The findings of this study suggested that eEF2K likely contributes to neuronal function by regulating the synthesis of microtubule-related proteins. PMID: 26485687
    7. eEF2K mediates PDGF-BB-induced SMCs proliferation and migration through activating ERK, Akt, p38 and HSP27 signals in a calmodulin-dependent manner PMID: 25069823
    8. Results show that eEF2K is rapidly activated in response to acidosis in cells, an effect that is followed by its downregulation. PMID: 25776553
    9. Data show that eEF2K is activated during hypoxia or upon inhibition of prolyl hydroxylases and inhibited by its hydroxylation on a highly conserved proline residue, restricting its activity during normoxia. PMID: 25755286
    10. data suggest that achieving an active conformation, rather than eEF2K activity per se, is required for its susceptibility to degradation. PMID: 25670349
    11. Because eEF2K activity in cells of the hematopoietic compartment contributes to atherosclerosis development, drugs inhibiting eEF2K might have a beneficial effect in treatment of atherosclerosis. PMID: 25475470
    12. Loss of eEF2K leads to increase in abnormal antral follicles and unhealthy oocytes. PMID: 24582807
    13. Data highlight a conserved role for eEF2K in protecting cells from nutrient deprivation and in conferring tumor cell adaptation to metabolic stress. PMID: 23706743
    14. Adequate levels of eEF2K phosphorylation are essential for cortical-dependent associative learning; malfunction of memory processing at the systems level can lead to associative memory impairment. PMID: 22366775
    15. Resveratrol inhibits the proliferation and migration of vascular endothelial cells by activating eukaryotic elongation factor-2 kinase. PMID: 20472894
    16. During late embryonic and early postnatal stages, elongation factor 2 kinase is up-regulated in the cerebral cortex and hippocampal pyramidal cell layer. PMID: 12213630

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  • 蛋白家族：
    Protein kinase superfamily, Alpha-type protein kinase family
  • 组织特异性：
    Ubiquitously expressed. Particularly abundant in skeletal muscle and heart.
  • 数据库链接：

    KEGG: mmu:13631

    STRING: 10090.ENSMUSP00000046595

    UniGene: Mm.25997