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  4. Recombinant Mouse Erythropoietin (Epo)

Recombinant Mouse Erythropoietin (Epo)

  • 货号：
    CSB-YP007743MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP007743MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP007743MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP007743MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP007743MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    EpoErythropoietin
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    27-192
  • 氨基酸序列
    APPR LICDSRVLER YILEAKEAEN VTMGCAEGPR LSENITVPDT KVNFYAWKRM EVEEQAIEVW QGLSLLSEAI LQAQALLANS SQPPETLQLH IDKAISGLRS LTSLLRVLGA QKELMSPPDT TPPAPLRTLT VDTFCKLFRV YANFLRGKLK LYTGEVCRRG DR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass. Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3.
  • 基因功能参考文献：
    1. Increases in plasma erythropoietin and erythropoietin receptor activation are mechanisms implicated in the increase of plasma FGF23 in acute kidney injury. PMID: 29395333
    2. Compared to wild type (WT) animals, Epo-TAg(h) female mice exhibited higher ventilation in hypoxia. However, when data were separated into luteal and follicular phases of the estrous cycle, basal ventilation and hypoxic ventilation were not different in both mice strains. Experiments with mifepristone (progesterone receptor antagonist) suggest that this effect is independent from the respiratory effects of progesterone. PMID: 28735074
    3. this study revealed a new mechanism wherein EPO alleviates hepatic steatosis by activating autophagy via SIRT1-dependent deacetylation of LC3. PMID: 29522896
    4. This study suggests that moderate elevated brain Epo levels provide clinically significant neuroprotection in experimental autoimmune encephalomyelitis without modulation of the immune response making a significant contribution. PMID: 29029628
    5. The functional role of signal cascades involved in the production of erythropoietin by T cells is determined by the stage of the common adaptation syndrome. PMID: 28744650
    6. data identify the PHD2:HIF-2alpha:EPO axis as a so far unknown regulator of osteohematology by controlling bone homeostasis. PMID: 27082941
    7. Data indicated the possible involvement of Jak2/STAT3/STAT6 pathway in the augmentation of EPO on M2 polarization. These results improved the understanding of the immunoregulatory capacity of EPO on macrophages, which might optimize the therapeutic modalities of EPO. PMID: 28383559
    8. The lack of effect of erythropoietin on hepcidin expression in mask mice can not be explained by changes in erythroferrone synthesis, as splenic erythroferrone content increased after erythropoietin administration in both C57BL/6 and mask mice. PMID: 29073189
    9. EPO expression in myoblasts and myotubes is increased by hypoxia and exercise PMID: 26885637
    10. these findings define a cis-regulatory enhancer network for Epo signaling during erythropoiesis, and provide the framework for future studies involving the interplay of epigenetics and Epo signaling. PMID: 28410882
    11. FOXD1 lineage renal interstitial cells consist of distinct subpopulations that differ in their responsiveness to Phd2 inactivation and thus regulation of HIF-2 activity and EPO production under hypoxia or conditions of pharmacologic or genetic PHD inactivation. PMID: 27088801
    12. This study suggests a possible role of EPO in embryonic endodermal development and a new agent for directing the differentiation into endodermal lineages like pancreatic beta-cells. PMID: 28298334
    13. Smad1 and Smad5 have overlapping functions to govern hepcidin transcription. Moreover, erythropoietin and erythroferrone target Smad1/5 signaling and require Smad1/5 to suppress hepcidin expression. PMID: 28438754
    14. EPO role in the glucose homeostasis, thermogenesis and endocrine function of classical brown adipose tissue PMID: 28288167
    15. Epo transcription in brain pericytes was HIF-2 dependent and cocontrolled by PHD2 and PHD3, oxygen- and 2-oxoglutarate-dependent prolyl-4-hydroxylases that regulate HIF activity. PMID: 27683416
    16. This study showed that polycythemia alone and increased levels of plasma Epo blunt the hypercapnic ventilatory response (HCVR); mice with an augmented level of cerebral Epo also had a decreased HCVR. PMID: 26936784
    17. Epo gene regulation in EPO-producing cells is a complex process that utilizes multiple regulatory influences. PMID: 27920250
    18. this study shows that EPO could directly promote tumor progression via EPO receptor-expressing macrophages PMID: 27262376
    19. pharmacologic downstream inhibition of the Bmp-Smad pathway by dorsomorphin, which targets the BMP receptors, improves the hepcidin responsiveness to EPO in Tmprss6 KO mice. PMID: 26755707
    20. In mice, inflammation increases blood levels of MIR122, which reduces expression of Epo in the kidney. PMID: 27477940
    21. findings reveal a novel function of LRRK2 in regulating EPO expression and imply a potentially novel relationship between PD genes and hematopoiesis. PMID: 27872856
    22. PI3K, MAPK/ERK 1/2, and p38-signaling proteins are not the main regulators of local production of erythropoietin after 30% loss of circulating blood volume. PMID: 27878722
    23. The here presented data unearthed EPO-dependent erythroferrone expression acts as an erythropoiesis-driven regulator of iron metabolism under phenylhydrazine-induced hemolytic anemia. PMID: 27067488
    24. DNA methyltransferase inhibition restores erythropoietin production in fibrotic murine kidneys PMID: 26731474
    25. cell-intrinsic role in macrophage signaling enhancing apoptotic cell clearance PMID: 26872696
    26. Midazolam suppresses hypoxia-induced up-regulation of EPO in brain. PMID: 26001375
    27. erythropoietin activates AKT, which phosphorylates GATA-1 at Ser310, thereby increasing GATA-1 affinity for FOG-1 PMID: 26680303
    28. a novel RIPC mechanism in which inhibition of infarct size by RIPC is produced through the renal nerve-mediated reduction of RBF associated with activation of the HIF1alpha-EPO pathway. PMID: 25833080
    29. Our data suggest that EPO-alpha and EPO-Z are not biosimilars for the wound healing effects. The higher efficacy of EPO-alpha might be likely due to its different conformational structure leading to a more efficient cell proliferation and skin remodelling. PMID: 26146639
    30. this study proposes a chemically regulated system of erythropoiesis that can be used as part of a peroral therapeutic strategy to treat Epo-deficiency anemia and provides us with the molecular mechanisms explaining the activation of the Epo-EpoR system PMID: 25790231
    31. Report cryopreservation of microencapsulated murine mesenchymal stem cells genetically engineered to secrete erythropoietin. PMID: 25708005
    32. EPO enhances chondrogenic and angiogenic responses during bone repair. PMID: 25003898
    33. findings are that chronic central Epo overexpression stimulates central breathing activity during hypoxia at early post-natal ages; results also suggest that erythropoietin accelerates the maturation of the newborn respiratory network and its response to hypoxia PMID: 24914467
    34. Decreased erythropoietin and lower erythropoietic activity in IL-10-deficient mice appears partially independent of the microbiota because spleen size was not significantly affected by cohousing the IL-10 knockout mice with the wild-type animals. PMID: 24973448
    35. In situ hybridization showed mRNA expression of erythropoietin in proximal convoluted tubules, distal convoluted tubules and cortical collecting ducts but not in the peritubular cells under normal conditions. PMID: 24832733
    36. The indispensable roles of Epo in primitive erythropoiesis.Erythropoietin production in neuroepithelial and neural crest cells during primitive erythropoiesis. PMID: 24309470
    37. Erythropoietin production in response to iron deficiency is partially modulated by angiotensin II type 1a receptor. PMID: 23398821
    38. Epo has a lineage instructive role in vivo, through suppression of non-erythroid fate options, demonstrating the ability of a cytokine to systematically bias successive lineage choices in favor of the generation of a specific cell type. PMID: 24493804
    39. Hypoxia signal, stimulated erythropoietin, which affected iron absorption by stabilizing duodenal FPN. PMID: 23656253
    40. erythropoietin contributes to skeletal muscle fiber programming and metabolism, and increases PGC-1alpha and AMPK activity during muscle development directly to affect the proportion of slow/fast twitch myofibers in mature skeletal muscle. PMID: 23523698
    41. Inherited super-anaemic mice (ISAM) were generated as a mouse model of adult-onset anaemia caused by erythropoietin deficiency. PMID: 23727690
    42. The phenotypic transition of renal Epo-producing cells to myofibroblasts, modulated by inflammatory molecules, underlies the connection between anemia and renal fibrosis in chronic kidney disease. PMID: 23833259
    43. Epo could be released by cardiomyocytes and that this source of Epo may be important for cardiac adaptation to hypoxia PMID: 23333855
    44. deletion of Vhl shifts the phenotype of juxtaglomerular cells from a renin- to erythropoietin-secreting cell type, presumably in response to HIF-2 accumulation PMID: 23393316
    45. The observed alterations in the muscle transcriptome suggest that physiological concentrations of EPO exert both direct and indirect muscle-protecting effects during exercise. PMID: 22748015
    46. These findings reveal a role of endogenous EPO/EPOR for cognition, at least in schizophrenic patients. PMID: 22669473
    47. High erythropoietin expression is associated with chronic hypoxia. PMID: 22879008
    48. RhEPO up-regulates the expression of FN and induced glomerular mesangial cells hypertrophy PMID: 22801437
    49. Data show it is feasible to reactivate hepatic erythropoietin production using systemically delivered siRNAs targeting the EglN1, EglN2 and EglN3 prolyl hydroxylase mRNA specifically in the liver. PMID: 22611156
    50. a novel role for GATA-4 and TAL1 to affect skeletal myogenic differentiation and EPO response via cross-talk with Sirt1. PMID: 22773876

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    EPO/TPO family
  • 组织特异性：
    Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
  • 数据库链接：

    KEGG: mmu:13856

    STRING: 10090.ENSMUSP00000031723

    UniGene: Mm.349116