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  4. Recombinant Mouse Eomesodermin homolog (Eomes)

Recombinant Mouse Eomesodermin homolog (Eomes)

  • 中文名称：
    小鼠Eomes重组蛋白
  • 货号：
    CSB-YP007701MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Eomes重组蛋白
  • 货号：
    CSB-EP007701MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Eomes重组蛋白
  • 货号：
    CSB-EP007701MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Eomes重组蛋白
  • 货号：
    CSB-BP007701MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Eomes重组蛋白
  • 货号：
    CSB-MP007701MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Eomes
  • Uniprot No.：
  • 别名：
    Eomes; Tbr2Eomesodermin homolog; T-box brain protein 2; T-brain-2; TBR-2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-707
  • 氨基酸序列
    MQLGEQLLVS SVNLPGAHFY SLESARGGGG GGGGGGGGGG GSVSLLPGAA PSPQRLDLDK ASKKFPGSLP CQAGSAEPAG AGAGAPAAML SDADAGDTFG STSAVAKPGP PDGRKGSPCA EEELPSAATA AATARYSMDS LSSERYYLPS PGPQGSELAA PCSLFQYPAA AGAAHGPVYP ASNGARYPYG SMLPPGGFPA AVCPPARAQF GPAAGSGSGA GSSGGGAGGP GAYPYGQGSP LYGPYAGTSA AGSCGGLGGL GVPGSGFRAH VYLCNRPLWL KFHRHQTEMI ITKQGRRMFP FLSFNINGLN PTAHYNVFVE VVLADPNHWR FQGGKWVTCG KADNNMQGNK MYVHPESPNT GSHWMRQEIS FGKLKLTNNK GANNNNTQMI VLQSLHKYQP RLHIVEVTED GVEDLNEPSK TQTFTFSETQ FIAVTAYQNT DITQLKIDHN PFAKGFRDNY DSMYTASEND RLTPSPTDSP RSHQIVPGGR YGVQNFFPEP FVNTLPQARY YNGERTVPQT NGLLSPQQSE EVANPPQRWL VTPVQQPVTN KLDIGSYESE YTSSTLLPYG IKSLPLQTSH ALGYYPDPTF PAMAGWGGRG AYQRKMAAGL PWTSRMSPPV FPEDQLAKEK VKEEISSSWI ETPPSIKSLD SSDSGVYNSA CKRKRLSPST PSNGNSPPIK CEDINTEEYS KDTSKGMGAY YAFYTSP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes.
  • 基因功能参考文献：
    1. IL-12 promoted increasing T-bet and down regulating Eomes to promote the CD8+ T cell differentiation to memory T-cells in invasive pulmonary aspergillosis mouse model. PMID: 28924369
    2. These data indicate that TBR2 contributes to suppressing RA signaling in INPs, thereby enabling them to re-enter the cell cycle and delay neuronal differentiation. PMID: 29305158
    3. These data identify TBR2 as a major determinant of the INP-specific traits by regulating both genetic and epigenetic pathways. PMID: 27600842
    4. Tbr2 regulates the tempo of laminar fate implementation for all cortical layers. PMID: 27320921
    5. results revealed Eomes expression is restricted to distinct tissues and Vgamma subsets; gammadelta T cells expressing Eomes presented a CD44+-activated memory phenotype with increased expression of Ly6C and Il2rb and showed higher IFN-gamma production upon stimulation; Eomes expression could be induced in peripheral and thymic gammadelta T cells by IL-12 and in particular by IL-4 PMID: 28386934
    6. activation of the Eomes target genes Foxa2 and Lhx1 is associated with higher order chromatin reorganization. PMID: 28174238
    7. this study shows that TGF-beta promotes salivary gland innate lymphoid cells differentiation by suppressing Eomes PMID: 27156386
    8. The Foxo3-Eomes pathway is central to achieve the complete specialized gene program required for pathogenic Th1 cell differentiation. PMID: 27742544
    9. HDAC11-knockout T cells displayed enhanced proliferation, proinflammatory cytokine production, and effector molecule expression of Eomes and TBX21 transcription factors previously shown to regulate inflammatory cytokine and effector molecule production. PMID: 28550044
    10. Results indicated that Eomes bound to the Ifng promoter and multiple conserved noncoding sequences in stimulation-dependent and stimulation-independent manners. PMID: 26749212
    11. Demonstrate by using the T-box brain-2(Cre) mouse line that progenitor cells derived cells contribute 67.5% of glutamatergic but not GABAergic or astrocytic cells to all cortical layers including the earliest generated subplate zone. PMID: 24927931
    12. a critical role for IRF4 in regulating protective anti-viral CD8+ T cell responses by ensuring a balanced ratio of T-bet to Eomes PMID: 26714260
    13. Eomes expression is a key determinant of conventional NK versus Type 1 helper Innate Lymphoid Cell maturation following lineage specification. PMID: 26792802
    14. T-cell-specific deletion of the Eomes gene remarkably ameliorates the late-onset experimental autoimmune encephalomyelitis. PMID: 26436530
    15. find that while stimulation of CD27 in isolation drives weak Eomesodermin(hi) T-bet(lo) CD8(+) T-cell responses to OVA immunization PMID: 26461455
    16. The data identify a novel role for Eomes in CD4+ T cells and indicate that Eomes expression may act by limiting Foxp3 induction, which may contribute to the association of EOMES to susceptibility to multiple sclerosis. PMID: 26453746
    17. type I interferon signalling in CD8(+) T cells drives Eomes expression. PMID: 25953241
    18. TGF-beta/tbr2 receptor signaling pathway may be required for prevention of beta cell death after initial cell proliferation following pancreatectomy. PMID: 25871744
    19. Interleukin-4 regulates eomesodermin in CD8+ T cell development and differentiation PMID: 25207963
    20. Transgenic overexpression of Wnt7b decreased expression of Tbr2 in both progenitor cells and post-mitotic neurons. PMID: 24953933
    21. Inflammation in acute microbial infection induced T-bet and Eomes. It promoted effector differentiation of self-reactive T cells in tolerance-favoring conditions. LCMV induced higher Eomes expression, which even without T-bet, made effector cytokines. PMID: 24823810
    22. Tbr2 regulates intrinsically photosensitive retinal ganglion cells formation and survival. PMID: 25253855
    23. Tbr2 is essential for proper morphogenesis of the OB and SVZ-RMS, and is important for the generation of multiple lineages of glutamatergic olfactory bulb neurons. PMID: 24550175
    24. The data of this study demonstrated that Tbr2 acts to execute RGC type choice and/or survival in a set of RGCs that mediates light-induced subconscious behaviors. PMID: 24741035
    25. Eomes is a specific target repressed by TGF-beta via Smad4 and Smad3 in CD8(+) T cells. PMID: 24127404
    26. E4bp4 is required for commitment to the natural killer (NK) lineage and promotes NK development by directly regulating the expression of the downstream transcription factors Eomes and Id2. PMID: 24663216
    27. Findings indicate that the transcription factor Nfil3 (E4bp4) drives the formation of mature NK cells by inducing Eomes expression and reveal the differential requirements of NK cell subsets for Nfil3. PMID: 24532575
    28. Mutually exclusive expression of T-bet and Eomes drives the development of different NK cell lineages with complementary functions. PMID: 24516120
    29. Active STAT5 regulates T-bet and eomesodermin expression in CD8 T cells and imprints a T-bet-dependent Tc1 program with repressed IL-6/TGF-beta1 signaling. PMID: 24006458
    30. Consistent with N-ras coupling Eomes to T cell memory, retrovirally enforced expression of Eomes in N-ras-deficient CD8+ T cells effectively rescued their memory differentiation. PMID: 23776078
    31. Jmjd3 sequentially associates with two T-box factors, Tbx3 and Eomes to drive stem cell differentiation towards the definitive endoderm lineage. PMID: 23584530
    32. Eomesodermin (Eomes) plays an essential role in AVE recruitment, in part by directly activating the homeobox transcription factor Lhx1 PMID: 23651855
    33. Systemic 4-1BB activation induces a novel T cell phenotype driven by high expression of Eomesodermin. PMID: 23547098
    34. cortical areas and connections are shaped by sequential maps of regional identity, propagated by the Pax6 --> Eomes --> Tbr1 TF cascade PMID: 23431145
    35. These studies indicate that Tbr2 is explicitly required for morphogenesis of the DG and participates in multiple aspects of the intricate developmental process of this structure. PMID: 23447624
    36. Data indicate that IRF4 functions as a regulator of CD8+ T-cell differentiation via suppression of Eomes expression after TCR signaling via IL-2 inducible T-cell kinase (ITK). PMID: 23011795
    37. This study suggested that Tbr2 is required for the proper differentiation of mitral, tufted cells and for the establishment of functional neuronal circuitry in the OB, maintenance of excitatory-inhibitory balance crucial for odor information processing. PMID: 22745484
    38. Eomes expression converts the functions of fully differentiated Th cells toward those of CTL by eliciting perforin and fasl expression. PMID: 22425747
    39. Rapid development of exhaustion and down-regulation of eomesodermin limit the antitumor activity of adoptively transferred murine natural killer cells. PMID: 22544698
    40. These results place Eomes upstream of the Mesp1-dependent programme of cardiogenesis, and at the intersection of mesodermal and endodermal specification, depending on the levels of Activin/Nodal signalling. PMID: 22402664
    41. IL-4 induction of IFN-gamma was explained by its ability to induce Eomesodermin and T-bet transcription factors whose expression was further increased by CD3/CD28. PMID: 22078635
    42. These findings suggest that Tbr2 expression in INPs is critical for neuronal differentiation in the dentate gyrus and that INPs are an essential stage in the lineage from NSCs to new granule neurons in the dentate gyrus. PMID: 22553033
    43. Eomes expression in the gastrulating mouse embryo is likely to be regulated by a distinct cis-acting region located from 20.1 to 23.6 kb upstream of the transcriptional start site. PMID: 21763783
    44. mice lacking both transcription factors Eomesodermin (Eomes) and T-bet failed to develop NK cells PMID: 22261438
    45. IL-5 production in memory Th2 cells is regulated by Eomesodermin via the inhibition of GATA3 activity. PMID: 22118525
    46. Data suggest that stress-induced MAPK8/9 and PRKAA1/2 regulate transcription factors Eomes/HAND1 and ID2, respectively. Together this network mediates induction of CSH1 by stress. PMID: 21710638
    47. Data show that Eomes promotes formation of cardiovascular progenitors by directly activating the bHLH transcription factor gene Mesp1 upstream of the core cardiac transcriptional machinery. PMID: 21822279
    48. the current study identifies for the first time a specific CD134 plus CD137 costimulatory pathway and an intracellular mechanism relying on Eomesodermin that induces cytotoxic CD4 Th1 cells PMID: 21880986
    49. Data show that development of the CD8+ ILLs present in SH2 domain-containing leukocyte protein, 76-kDa Y145F mice require Eomes and PLZF. PMID: 21383242
    50. suppression of Eomes by TGF-beta via the JNK pathway is an important mechanism for Smad-independent Th17 cell differentiation PMID: 21600798

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  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Expressed in CD8+ T-cells.
  • 数据库链接：

    KEGG: mmu:13813

    STRING: 10090.ENSMUSP00000035020

    UniGene: Mm.200692