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  4. Recombinant Mouse E3 ubiquitin-protein ligase UHRF1 (Uhrf1)

Recombinant Mouse E3 ubiquitin-protein ligase UHRF1 (Uhrf1)

  • 中文名称：
    小鼠Uhrf1重组蛋白
  • 货号：
    CSB-YP848684MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Uhrf1重组蛋白
  • 货号：
    CSB-EP848684MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Uhrf1重组蛋白
  • 货号：
    CSB-EP848684MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Uhrf1重组蛋白
  • 货号：
    CSB-BP848684MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Uhrf1重组蛋白
  • 货号：
    CSB-MP848684MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Uhrf1
  • Uniprot No.：
  • 别名：
    Uhrf1; Np95E3 ubiquitin-protein ligase UHRF1; EC 2.3.2.27; Nuclear protein 95; Nuclear zinc finger protein Np95; RING-type E3 ubiquitin transferase UHRF1; Ubiquitin-like PHD and RING finger domain-containing protein 1; mUhrf1; Ubiquitin-like-containing PHD and RING finger domains protein 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-782
  • 氨基酸序列
    MWIQVRTMDG KETHTVNSLS RLTKVQELRK KIEEVFHVEP QLQRLFYRGK QMEDGHTLFD YDVRLNDTIQ LLVRQSLALP LSTKERDSEL SDSDSGYGVG HSESDKSSTH GEGAAEADDK TVWEDTDLGL YKVNEYVDVR DNIFGAWFEA QVVQVQKRAL SEDEPCSSSA VKTSEDDIMY HVKYDDYPEH GVDIVKAKNV RARARTVIPW ENLEVGQVVM ANYNVDYPRK RGFWYDVEIC RKRQTRTARE LYGNIRLLND SQLNNCRIMF VDEVLMIELP KERRPLIASP SQPPPALRNT GKSGPSCRFC KDDENKPCRK CACHVCGGRE APEKQLLCDE CDMAFHLYCL KPPLTSVPPE PEWYCPSCRT DSSEVVQAGE KLKESKKKAK MASATSSSRR DWGKGMACVG RTTECTIVPA NHFGPIPGVP VGTMWRFRVQ VSESGVHRPH VAGIHGRSND GAYSLVLAGG YEDDVDNGNY FTYTGSGGRD LSGNKRTAGQ SSDQKLTNNN RALALNCHSP INEKGAEAED WRQGKPVRVV RNMKGGKHSK YAPAEGNRYD GIYKVVKYWP ERGKSGFLVW RYLLRRDDTE PEPWTREGKD RTRQLGLTMQ YPEGYLEALA NKEKSRKRPA KALEQGPSSS KTGKSKQKST GPTLSSPRAS KKSKLEPYTL SEQQANLIKE DKGNAKLWDD VLTSLQDGPY QIFLSKVKEA FQCICCQELV FRPVTTVCQH NVCKDCLDRS FRAQVFSCPA CRFELDHSSP TRVNQPLQTI LNQLFPGYGS GR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

Regarding your protein CSB-MP848684MO, I'm interested to know if this protein can be made with an N-terminal GST AND 6His-tag – have you ever expressed this protein like this, or would you expect this tag to be suitable?
Thanks so much!

A:
Thanks for your inquiry.
Regarding CSB-MP848684MO, sorry, we haven't expressed this protein before, as you request N-terminal GST AND 6His-tag, should both of the tags be located at the N-terminal?
Do you mean the N-terminal 6xHis-GST-tagged? We have expressed other MP proteins with N-terminal GST-tagged and C-terminal 6xHis-tagged,
In theory, we can try to express this protein with N-terminal 6xHis-GST-tagged, but we are not sure about the success rate.
If it fails, we can provide it with other similar tag. In addition, since this protein itself is very large, if adding a large tag, it will make it much more difficult to express.
Do you accept the partial protein? If yes, please let us know which region you want to express.

靶点详情

  • 功能：
    Multidomain protein that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Specifically recognizes and binds hemimethylated DNA at replication forks via its YDG domain and recruits DNMT1 methyltransferase to ensure faithful propagation of the DNA methylation patterns through DNA replication. In addition to its role in maintenance of DNA methylation, also plays a key role in chromatin modification: through its tudor-like regions and PHD-type zinc fingers, specifically recognizes and binds histone H3 trimethylated at 'Lys-9' (H3K9me3) and unmethylated at 'Arg-2' (H3R2me0), respectively, and recruits chromatin proteins. Enriched in pericentric heterochromatin where it recruits different chromatin modifiers required for this chromatin replication. Also localizes to euchromatic regions where it negatively regulates transcription possibly by impacting DNA methylation and histone modifications. Has E3 ubiquitin-protein ligase activity by mediating the ubiquitination of target proteins such as histone H3 and PML. It is still unclear how E3 ubiquitin-protein ligase activity is related to its role in chromatin in vivo. Plays a role in DNA repair by cooperating with UHRF1 to ensure recruitment of FANCD2 to interstrand cross-links (ICLs) leading to FANCD2 activation ().
  • 基因功能参考文献：
    1. Epigenetic enhancement of the post-replicative DNA mismatch repair of mammalian genomes by a Hemi-(m)CpG-Np95-Dnmt1 axis has been demonstrated for humans and mice. PMID: 27886214
    2. These results indicate that Uhrf1 governs cell type-specific transcriptional regulation by controlling the genome-wide DNA methylation status and regulating consequent cell differentiation and skeletal maturation. PMID: 29180567
    3. UHRF1 ubiquitinates PAF15 at Lys 15 and Lys 24 and promotes its binding to PCNA during late S-phase PMID: 29055779
    4. Whole genome bisulfite sequencing revealed that blastocysts derived from KO oocytes have a greatly reduced level of CG methylation, suggesting that maternal UHRF1 is essential for maintaining CG methylation, particularly at the imprinting control regions, in preimplantation embryos. PMID: 28976982
    5. SETDB1 maintains silencing of intracisternal A particle, but in the absence of DNMT1, prolonged binding of NP95 to hemimethylated DNA transiently disrupts SETDB1-dependent H3K9me3 deposition. PMID: 27151458
    6. deletion of Uhrf1 lead to global DNA hypomethylation with a strong activation of the intracisternal A particle (IAP) family of endogenous retroviral elements, accompanied by an increase in 5-hydroxymethylcytosine PMID: 27798843
    7. Our results provide insights into the molecular basis of DNMT1 dysfunction in hereditary sensory and autonomic neuropathies with dementia and hearing loss patients and emphasize the importance of the TS domain in the regulation of DNA methylation in pluripotent and differentiating cells PMID: 28334952
    8. results identify UHRF1 as a novel HSP90 client protein and shed light on the regulation of UHRF1 stability and function. PMID: 27489107
    9. Uhrf1 regulation of the Akt-mTOR signaling pathway is required for invariant natural killer T cell development. PMID: 27050515
    10. Hemi-methylated DNA opens a closed conformation of UHRF1 to facilitate its H3 histone recognition. PMID: 27045799
    11. the histone modification status at the DMRs of imprinted genes may dictate whether DNA methylation could be restored upon UHRF1 re-expression PMID: 25900245
    12. UHRF1 is strongly up-regulated during liver regeneration independently of BIRC5. PMID: 24818710
    13. report identifies PARP1 as a novel modulator of two UHRF1-regulated heterochromatin-associated events: the accumulation of H4K20me3 and the clearance of DNMT1 PMID: 24782312
    14. Uhrf1 deficiency resulted in derepression of the gene (Cdkn1a) that encodes the cyclin-dependent kinase inhibitor p21 due to hypomethylation of its promoter region, which resulted in cell-cycle arrest of Treg cells. PMID: 24777532
    15. These findings show that apart from the targeting of DNMT1 to the replicated DNA UHRF1 increases the activity and specificity of DNMT1, thus exerting a multifaceted influence on the maintenance of DNA methylation. PMID: 24368767
    16. The DNA methyltransferase Dnmt1 directly interacts with the SET and RING finger-associated (SRA) domain of the multifunctional protein Uhrf1 to facilitate accession of the catalytic center to hemi-methylated DNA. PMID: 24253042
    17. UHRF1 can target DNMT1 for DNA maintenance methylation through binding either histone 3 lysine 9 methylation 2/3 or hemi-methylated CpG. PMID: 23463006
    18. The UHRF1 is not functionally redundant in DNA methylation maintenance and reveals the cell-cycle-dependent interaction between UHRF1 and DNMT1 as a key regulatory mechanism targeting DNMT1 for DNA methylation. PMID: 22064703
    19. results reveal that the disruption of Dnmt1/PCNA/UHRF1 interactions acts as an oncogenic event and that one of its signatures (i.e. the low level of mMTase activity) is a molecular biomarker associated with a poor prognosis in GBM patients PMID: 20613874
    20. Np95, Dnmt3a and Dnmt3b in mediating epigenetic silencing through histone modification followed by DNA methylation PMID: 19798101
    21. Np95 is specifically induced in terminally differentiated mouse 3T3 cells in vitro by adenovirus E1A proteins and is essential for S phase entry. PMID: 12058012
    22. NP95 functions as a common component in the multiple response pathways against DNA damage and replication arrest and thereby contributes to genomic stability. PMID: 12084726
    23. Np95 is a chromatin-associated ubiquitin ligase that binds to histones in vivo and in vitro PMID: 14993289
    24. Np95 is a new relevant protein involved in heterochromatin replication and formation. PMID: 17182844
    25. data suggest that UHRF1 may help recruit DNMT1 to hemimethylated DNA to facilitate faithful maintenance of DNA methylation PMID: 17673620
    26. Np95 is essential in vivo to maintain global and local DNA methylation and to repress transcription of retrotransposons and imprinted genes PMID: 17994007
    27. the PHD domain of Np95-ICBP90 contributes to the opening and/or stabilization of dense chromocenter structures to support the recruitment of modifying enzymes, like HDAC and DNMT1, required for the replication and formation of PH PMID: 18508923
    28. These findings link Eme1 with the replication-associated chromatin modifier functions of Np95 in the cellular response to DNA damage. PMID: 18692478
    29. Crystal structure; UHRF1 contains a previously unknown DNA-binding module and is the first example of a non-enzymatic, sequence-specific DNA-binding protein domain to use the base flipping mechanism to interact with DNA PMID: 18772888
    30. the crystal structures of the SET and RING-associated (SRA) domain of Uhrf1 in free and hemi-methylated DNA-bound states PMID: 18772891
    31. UHRF1 recruited and cooperated with G9a to inhibit the p21 promoter activity, which correlated with the elevated p21 protein level in murine Uhrf1-null embryonic stem cells. PMID: 19056828
    32. UHRF1 may bring two components (histones and DNA) carrying appropriate markers (on the tails of H3 and hemimethylated CpG sites) ready to be assembled into a nucleosome after replication PMID: 19077538
    33. UHRF1 inhibits the late phase of oligodendrocyte differentiation. PMID: 17065439

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  • 亚细胞定位：
    Nucleus. Note=Localizes to replication foci. Enriched in pericentric heterochromatin. Also localizes to euchromatic regions.
  • 组织特异性：
    Expressed in thymus, testis, spleen and lung. Within testis, expressed in almost all cells except elongated spermatids.
  • 数据库链接：

    KEGG: mmu:18140

    STRING: 10090.ENSMUSP00000001258

    UniGene: Mm.42196