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  4. Recombinant Mouse E3 ubiquitin-protein ligase NHLRC1 (Nhlrc1)

Recombinant Mouse E3 ubiquitin-protein ligase NHLRC1 (Nhlrc1)

  • 货号：
    CSB-YP807395MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP807395MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP807395MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP807395MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP807395MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Nhlrc1; Epm2b; E3 ubiquitin-protein ligase NHLRC1; EC 2.3.2.27; Malin; NHL repeat-containing protein 1; RING-type E3 ubiquitin transferase NHLRC1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-401
  • 氨基酸序列
    MGEEATAVAA AGVRPELVRE AEVSLLECKV CFERFGHWQQ RRPRNLPCGH VVCLACVAAL AHPRTLGLEC PFCRRACRAC DTSDCLPVLH LLELLGSTLH ASPAALSAAP FAPGTLTCYH AFGGWGTLVN PTGLALCPKT GRVVVVHDGK RRVKIFDSGG GGAHQFGEKG DAAHDVKYPL DVAVTNDCHV VVTDAGDCSL KVFDFFGQIK LVVGKQFSLP WGVEITPHNG VLVTDAEAGT LHLLEADFPE GVLRRIERLQ AHLCSPRGLA VSWLTGAIAV LEHPCAFGRT GCNNTRVKVF NSTMQLIGQV DSFGLNLLFP SKVTASAVTF DHQGNVIVAD TSGPAIVCLG KPEEFPALKP IITHGLSRPV ALAFTKENSL LVLDTASHSI KVFKVMEGNG G
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    E3 ubiquitin-protein ligase. Together with the phosphatase EPM2A/laforin, appears to be involved in the clearance of toxic polyglucosan and protein aggregates via multiple pathways. In complex with EPM2A/laforin and HSP70, suppresses the cellular toxicity of misfolded proteins by promoting their degradation through the ubiquitin-proteasome system (UPS). Ubiquitinates the glycogen-targeting protein phosphatase subunits PPP1R3C/PTG and PPP1R3D in a laforin-dependent manner and targets them for proteasome-dependent degradation, thus decreasing glycogen accumulation. Polyubiquitinates EPM2A/laforin and ubiquitinates AGL and targets them for proteasome-dependent degradation. Also promotes proteasome-independent protein degradation through the macroautophagy pathway.
  • 基因功能参考文献：
    1. The present study analyzes possible inflammatory responses in the mouse lines Epm2a (-/-) (laforin knock-out) and Epm2b (-/-) (malin knock-out) with disease progression. PMID: 27041370
    2. Loss of malin leads to reduced proteasomal activity in the heat-shocked cells. PMID: 27975203
    3. This study also suggests a malin function independent of laforin, possibly in lysosomal biogenesis and/or lysosomal glycogen disposal. PMID: 24914213
    4. Results indicate that malin has no effect on whole-body glucose metabolism and insulin sensitivity. PMID: 22186021
    5. Dysfunction of autophagy is a common feature of both laforin- and malin-deficient mice. PMID: 22361617
    6. malin functions to regulate laforin and that malin deficiency at least in part causes LB and LD through increased laforin binding to glycogen. PMID: 22669944
    7. Results show that a functional laforin-malin complex plays a critical role in disrupting Lafora bodies and relieving endoplasmic reticulum stres. PMID: 22578008
    8. Motor coordination, activity impairment, and memory deficits progressively increase with age in Epm2b deficient mice. PMID: 22487859
    9. Data conclude that EPM2B functions to maintain laforin associated with soluble glycogen and that its absence causes sequestration of laforin to an insoluble polysaccharide fraction where it is functionally inert. PMID: 20538597
    10. laforin and malin play a role protecting cells from ER-stress, likely contributing to the elimination of unfolded proteins PMID: 19529779

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  • 亚细胞定位：
    Endoplasmic reticulum. Nucleus.
  • 数据库链接：

    KEGG: mmu:105193

    STRING: 10090.ENSMUSP00000054990

    UniGene: Mm.25593