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  4. Recombinant Mouse E3 ubiquitin-protein ligase MGRN1 (Mgrn1)

Recombinant Mouse E3 ubiquitin-protein ligase MGRN1 (Mgrn1)

  • 中文名称：
    小鼠Mgrn1重组蛋白
  • 货号：
    CSB-YP880460MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Mgrn1重组蛋白
  • 货号：
    CSB-EP880460MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Mgrn1重组蛋白
  • 货号：
    CSB-EP880460MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Mgrn1重组蛋白
  • 货号：
    CSB-BP880460MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Mgrn1重组蛋白
  • 货号：
    CSB-MP880460MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Mgrn1; Kiaa0544E3 ubiquitin-protein ligase MGRN1; EC 2.3.2.27; Mahogunin RING finger protein 1; RING-type E3 ubiquitin transferase MGRN1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-532
  • 氨基酸序列
    GSILSRRIA GVEDIDIQAN SAYRYPPKSG NYFASHFFMG GEKFDTPHPE GYLFGENMDL NFLGSRPVQF PYVTPAPHEP VKTLRSLVNI RKDSLRLVRY KEDADSPTED GEKPRVLYSL EFTFDADARV AITIYCQAVE ELVNGVAVYS CKNPSLQSET VHYKRGVSQQ FSLPSFKIDF SEWKDDELNF DLDRGVFPVV IQAVVDEGDV VEVTGHAHVL LAAFEKHVDG SFSVKPLKQK QIVDRVSYLL QEIYGIENKN NQETKPSDDE NSDNSSECVV CLSDLRDTLI LPCRHLCLCT SCADTLRYQA NNCPICRLPF RALLQIRAVR KKPGALSPIS FSPVLAQSVD HDEHSSSDSI PPGYEPISLL EALNGLRAVS PAIPSAPLYE EITYSGISDG LSQASCPLAG LDRIMESGLQ KGKTQSKSPD STLRSPSFPI HEEDEEKLSE DSDAPLPPSG VELVLRESSS PESFGTEEGD EPSLKQGSRV PSIDDVLQDG SPQHHGCSQP VPPADIYLPA LGPESCSVGI EE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    E3 ubiquitin-protein ligase. Mediates TSG101 monoubiquitination at multiple sites. Plays a role in the regulation of endosome-to-lysosome trafficking. Impairs MC1R- and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production. Does not inhibit ADRB2-signaling. Does not promote MC1R ubiquitination. Acts also as a negative regulator of hedgehog signaling.
  • 基因功能参考文献：
    1. Mgrn1 can negatively regulate signaling through the Hedgehog pathway by reducing cell-surface and ciliary levels of Smoothened, an oncoprotein that transmits the Hedgehog signal across the membrane. PMID: 29290584
    2. With aging, the neuroprotective e3 ligase MGRN1 relocates from the cytosol to the nucleus of neurons, where it associates with chromatin and potentiates the cellular response to proteotoxic stress. This nuclear shift is due to a proteasome impairment dependent increase of a monoubiquitinated form of MGRN1. PMID: 28475871
    3. Findings suggest that mahogunin ring finger-1 contributes to the clearance of toxic mutant copper-zinc superoxide dismutase inclusions likely through autophagic pathway. PMID: 26607786
    4. Mahogunin-mediated alpha-tubulin ubiquitination via noncanonical K6 linkage regulates microtubule stability and mitotic spindle orientation. PMID: 24556679
    5. This study indicates the mahogunin deficiency results in the infertility of male mice, disruption of hormones secretion and impaired active progressive motility. PMID: 23210986
    6. ATRN also has no effect on prion disease onset or progression and discuss possible mechanisms that could cause vacuolation of the central nervous system in Mgrn1 and Atrn null mutant mice PMID: 23787699
    7. pigment-type switching likely requires MGRN1's ubiquitin ligase activity but not its ability to bind TSG101 or NEDD4. PMID: 23253940
    8. Levels of the Mahogunin Ring Finger 1 E3 ubiquitin ligase do not influence prion disease. PMID: 23383230
    9. positional cloning of mahoganoid, encoding a novel 494-amino acid protein containing a C3HC4 RING (really interesting new gene) domain that may function as an E3 ubiquitin ligase PMID: 12438443
    10. a null mutation for mahoganoid causes a similar age-dependent neuropathology that includes many features of prion diseases but without accumulation of protease-resistant prion protein PMID: 12560552
    11. Mahoganoid mutation (Mgrn1md) on insulin sensitivity suggests that Mgrn1md may be increasing insulin sensitivity via the hypothalamic melanocortin-3 receptor pathway. PMID: 16638826
    12. Mgrn1 mutant embryos show abnormal expression of asymmetrically expressed genes involved in LR patterning. PMID: 17075880
    13. Our results indicate that reduced levels of MGRN1 cause the pigmentation phenotypes of Mgrn1 mutant mice and that there are no significant differences in the function of the four MGRN1 isoforms in pigment-type switching. PMID: 17083490
    14. Loss of E3 ubiquitin ligase MGRN1 in mutant mice causes neurodegeneration via accumulation of its substrates. PMID: 17720281
    15. data show that different Mgrn1 isoforms are not functionally equivalent in vivo and that the presence of only isoform I or III is sufficient for normal development, pigmentation, and neuronal integrity PMID: 19422019
    16. study shows ASIP-MC1R signaling includes a cAMP-independent pathway through attractin and mahogunin, while the known cAMP-dependent component requires neither attractin nor mahogunin PMID: 19493315
    17. Study shows that both (Ctm)PrP and cyPrP can interact with and disrupt the function of Mahogunin (Mgrn), a cytosolic ubiquitin ligase whose loss causes spongiform neurodegeneration. PMID: 19524515

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  • 亚细胞定位：
    Early endosome.; [Isoform 1]: Cytoplasm. Cell membrane.; [Isoform 5]: Cytoplasm. Nucleus. Note=In the cytoplasm, predominantly localized to the perinuclear region and discrete vesicular structures. In the nucleus, broadly distributed, but excluded from nucleoli.
  • 组织特异性：
    Widely expressed, with highest levels in brain, heart, kidney and liver. In the CNS, especially prominent in the Purkinje cells of the cerebellum. In the skin, expressed in the basal layer of the epidermis and hair follicles, primarily in the outer root s
  • 数据库链接：

    KEGG: mmu:17237

    STRING: 10090.ENSMUSP00000023159

    UniGene: Mm.291326