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  4. Recombinant Mouse E3 ubiquitin/ISG15 ligase TRIM25 (Trim25)

Recombinant Mouse E3 ubiquitin/ISG15 ligase TRIM25 (Trim25)

  • 货号：
    CSB-YP733787MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP733787MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP733787MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP733787MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP733787MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Trim25; Efp; Zfp147; Znf147; E3 ubiquitin/ISG15 ligase TRIM25; EC 6.3.2.n3; Estrogen-responsive finger protein; RING-type E3 ubiquitin transferase; EC 2.3.2.27; RING-type E3 ubiquitin transferase TRIM25; Tripartite motif-containing protein 25; Ubiquitin/ISG15-conjugating enzyme TRIM25; Zinc finger protein 147
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-634
  • 氨基酸序列
    MAELNPLAEE LSCSVCLELF KEPVTTPCGH NFCMSCLDET WVVQGPPYRC PQCRKVYQVR PQLQKNTVMC AVVEQFLQAE QARTPVDDWT PPARFSASSA ATQVACDHCL TEIAVKTCLV CMASFCQEHL RPHFDSPAFQ DHPLQSPIRD LLRRKCTQHN RLRELFCPEH GECICHICLV EHKTCSPTTL SQASADLEYK LRNKLTIMHS HINGATKALE DVRSKQQCVQ DSMKRKMEQL RQEYMEMKAV IDAAETSSLR KLKEEEKRVY GKFDTIYQVL VKKKSEMQKL KAEVELIMDK GDEFEFLEKA AKLQGESTKP VYIPKIDLDH DLIMGIYQGA ADLKSELKHS IKKLQKKSEE HNGSGNKGDQ TQSTFKPVQP SKKTIQEKKT KKTPVAPGPP SHFSPNKLPT FGAPGQSLDS KATSPDAAPK ASAAQPDSVG VKAKVLENFL TKSRTELLEY FVKVIFDYNT AHNKVSLSNK YTTASVSDGL QHYRSHPQRF TYCSQVLGLH CYKNGIHYWE VELQKNNFCG VGICYGSMER QGPESRLGRN PNSWCVEWFN NKISAWHNNV EKTLPSTKAT RVGVLLNCDH GFVIFFAVTE KVHLMYKFKV DFTEALYPAF WVFSAGTTLS ICSK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58 and IFIH1. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region and may play a role in signal transduction that leads to the production of interferons in response to viral infection. Mediates 'Lys-63'-linked polyubiquitination of IFIH1. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3. Plays a role in promoting the restart of stalled replication forks via interaction with the KHDC3-OOEP scaffold and subsequent ubiquitination of BLM, resulting in the recruitment and retainment of BLM at DNA replication forks.
  • 基因功能参考文献：
    1. TRIM25 as a key determinant of breast cancer metastasis. PMID: 28813674
    2. Studies indicated that mice with disrupted Trim25 were viable and fertile, uterine response to oestrogen was greatly attenuated and the uteri were underdeveloped. PMID: 27528750
    3. Two key residues (Asp(488) and Trp(621)) in the TRIM25 B30.2 domain are critical for binding to the RIG-I CARDs. PMID: 24015671
    4. Two well-known E3 ubiquitin ligases, Trim25 (also called Efp) and Trim71 (also called Lin41), are validated as RNA-binding proteins, revealing a potential link between RNA biology and protein-modification pathways. PMID: 23912277
  • 亚细胞定位：
    Cytoplasm. Cytoplasm, Stress granule. Nucleus.
  • 组织特异性：
    Ubiquitous.
  • 数据库链接：

    KEGG: mmu:217069

    STRING: 10090.ENSMUSP00000103528

    UniGene: Mm.248445