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  4. Recombinant Mouse E3 SUMO-protein ligase PIAS3 (Pias3)

Recombinant Mouse E3 SUMO-protein ligase PIAS3 (Pias3)

  • 货号：
    CSB-YP017959MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP017959MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP017959MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP017959MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP017959MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Pias3; E3 SUMO-protein ligase PIAS3; EC 2.3.2.-; E3 SUMO-protein transferase PIAS3; Protein inhibitor of activated STAT protein 3
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-628
  • 氨基酸序列
    MAELGELKHM VMSFRVSELQ VLLGFAGRNK SGRKHELLAK ALHLLKSSCA PSVQMKIKEL YRRRFPRKTL GPSDLSLLSL PPGTSPVGSP GPLAPIPPTL LTPGTLLGPK REVDMHPPLP QPVHPDVTMK PLPFYEVYGE LIRPTTLAST SSQRFEEAHF TFALTPQQLQ QILTSREVLP GAKCDYTIQV QLRFCLCETS CPQEDYFPPN LFVKVNGKLC PLPGYLPPTK NGAEPKRPSR PINITPLARL SATVPNTIVV NWSSEFGRNY SLSVYLVRQL TAGTLLQKLR AKGIRNPDHS RALIKEKLTA DPDSEVATTS LRVSLMCPLG KMRLTVPCRA LTCAHLQSFD AALYLQMNEK KPTWTCPVCD KKAPYESLII DGLFMEILNS CSDCDEIQFM EDGSWCPMKP KKEASEVCPP PGYGLDGLQY SAVQEGIQPE SKKRVEVIDL TIESSSDEED LPPTKKHCPV TSAAIPALPG SKGALTSGHQ PSSVLRSPAM GTLGSDFLSS LPLHEYPPAF PLGADIQGLD LFSFLQTESQ HYGPSVITSL DEQDTLGHFF QYRGTPSHFL GPLAPTLGSS HRSSTPAPPP GRVSSIVAPG SSLREGHGGP LPSGPSLTGC RSDVISLD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Repressor of STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Repressor of MITF transcriptional activity. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Sumoylates CCAR2 which promotes its interaction with SIRT1. Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus.
  • 基因功能参考文献：
    1. Results show that PIAS3 levels are reduced in a mouse model of atherosclerotic lesions and that its expression decreases in conjunction with increases in interleukin-6 expression and disease severity. These findings provide evidence that results indicate that PIAS3 is a critical repressor of atherosclerosis progression. PMID: 27845432
    2. Data indicate that PIAS3 (protein inhibitor of activated STAT3) interaction modulates EKLF (erythroid Kruppel-like factor) activity in a promoter-dependent and SUMO-independent manner. PMID: 25713074
    3. PIAS3 suppresses acute graft-versus-host disease by modulating effector T and B cell subsets through inhibition of STAT3 activation. PMID: 24718277
    4. These data indicate that tachypacing decreased ATBF1, leading to enhanced STAT3 DNA-binding activity due to the reduced formation of a binary complex of ATBF1 and PIAS3. PMID: 24857987
    5. MRL/lpr mice have significantly increased expressions of STAT3 mRNA and protein and decreased expression of mRNA PIAS3 in the kidneys compared with BALB/C mice. PMID: 22699062
    6. L97A, R99N and R99Q mutations of the PINIT domain (PIAS3(85-272) ) were found to abrogate binding to STAT3, suggesting that these residues were part of a potential binding surface. PMID: 21812053
    7. Our results indicate that Pias3-dependent SUMOylation of photoreceptor-specific transcription factors is a common mechanism that controls both rod and cone photoreceptor subtype specification, regulating distinct molecular targets in the two cell types PMID: 20729845
    8. there is an interaction between Zimp7 and PIAS proteins with higher preference for PIAS3, in androgen receptor-mediated transcription PMID: 20159969
    9. results indicate a potential role of PIAS3 as transcriptional modulator of TIF2-mediated signalling PMID: 12208521
    10. role in inducing SUMO-1 modification and transcriptional repression of IRF-1 PMID: 12387893
    11. identified a novel conserved domain of 180 residues in PIAS proteins and showed that its 'PINIT' motif as well as other conserved motifs (in the SAP box and in the RING domain) are independently involved in nuclear retention of PIAS3L PMID: 14596924
    12. Zip domain is the region of MITF that is involved in the direct interaction between MITF and PIAS3. PMID: 14645519
    13. The interplay between Mitf, Pias3, and Stat3 was studied in mast cell and melanocytes. PMID: 15572665
    14. Taken together, these data suggest that PIAS3 may play an inhibitory role in adipogenesis by modulating insulin-activated transcriptional activation events. PMID: 16329991
    15. The overexpression of PIAS3 attenuates osteoclast formation and down-regulates the expression of NFATc1 and osteoclast-associated receptor (OSCAR), which are important modulators in osteoclastogenesis. PMID: 17442941
    16. PIAS3 is a new regulator of ATF1 that regulates the ARE-mediated transcription of the ferritin H gene PMID: 17565989
    17. PIAS3 negatively regulates RANKL-mediated osteoclastogenesis directly in osteoclast precursors and indirectly via osteoblasts. PMID: 18952894
    18. Our data thus identify Pias3-mediated SUMOylation of photoreceptor-specific transcription factors as a key mechanism of rod specification. PMID: 19186166

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  • 亚细胞定位：
    Cytoplasm. Nucleus. Nucleus speckle.
  • 蛋白家族：
    PIAS family
  • 组织特异性：
    Expressed in kidney, heart, spleen, brain and cerebellum; weak expression, if any, in liver and lung.
  • 数据库链接：

    KEGG: mmu:229615

    STRING: 10090.ENSMUSP00000069259

    UniGene: Mm.1635