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  4. Recombinant Mouse Dual specificity protein phosphatase CDC14B (Cdc14b)

Recombinant Mouse Dual specificity protein phosphatase CDC14B (Cdc14b)

  • 货号：
    CSB-YP740782MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP740782MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP740782MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP740782MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP740782MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Cdc14bDual specificity protein phosphatase CDC14B; EC 3.1.3.16; EC 3.1.3.48; CDC14 cell division cycle 14 homolog B
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-485
  • 氨基酸序列
    MKRKSERRSA WATAPPCSRR SSSSSPGVKK SRSSTPQELH RLEQQDDLYL DITDRLCFAI LYSRPKSATN EHYFSIDNEL EYENFYADFG PLNLAMVYRY CCKINKKLKS ITMLRKKIIH FTGTDQRKQA NAAFLVGCYM VIYLGRTPED AYRTLIFGDT AYIPFRDAAY GSCSFYITLL DCFHAVKKAM QYGFFNFNSF NLDEYEHYEK AENGDFNWII PERFLAFCGP HSRSRLESGY HQHSPETYIP YFKNHNVTTI IRLNKRMYDA KRFTDAGFDH HDLFFPDGST PAESIVQEFL DICENVKGAI AVHCKAGLGR TGTLIGCYLM KHYRMTAAES IAWLRICRPG SVIGPQQQFL VMKQSSLWLE GDYFRQKLRG QENGPLREAF SKHLSDADDL SLNGLENQDN QEPEPYSDDD EVSGMTQGDR LRALKSRRQP KASAIPLTCP LAVLTSALCS VAIWWIVCDY ILPTLLFCLD GFRTQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Dual-specificity phosphatase involved in DNA damage response. Essential regulator of the G2 DNA damage checkpoint: following DNA damage, translocates to the nucleus and dephosphorylates FZR1/CDH1, a key activator of the anaphase promoting complex/cyclosome (APC/C). Dephosphorylates SIRT2 around early anaphase. Dephosphorylation of FZR1/CDH1 activates the APC/C, leading to the ubiquitination of PLK1, preventing entry into mitosis. Preferentially dephosphorylates proteins modified by proline-directed kinases.
  • 基因功能参考文献：
    1. Cdc14B knockout (Cdc14B(-/-)) mouse embryonic fibroblasts showed defects in repairing ionizing radiation-induced DNA double-strand breaks, which occurred only at late passages when Cdc14A levels were low. PMID: 26283732
    2. Cdc14b regulates mammalian RNA polymerase II and represses cell cycle transcription PMID: 22355704
    3. Cdc14B has oncogenic activity in mammals and point to the Ras-MAP kinase pathway as a major effector pathway during oncogenic transformation. PMID: 21502810
    4. Cdc14b is required for efficient DNA damage repair. PMID: 21262768
    5. CDC14B is a negative regulator of the 1-to-2-cell transition and of zygotic genome activation in mouse embryogenesis. PMID: 19923902
    6. CDC14B is a negative regulator of meiotic resumption and may regulate meiosis I in mouse oocytes. PMID: 19129509

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  • 亚细胞定位：
    Nucleus, nucleolus. Nucleus, nucleoplasm.
  • 蛋白家族：
    Protein-tyrosine phosphatase family, Non-receptor class CDC14 subfamily
  • 数据库链接：

    KEGG: mmu:218294

    STRING: 10090.ENSMUSP00000046003

    UniGene: Mm.25335