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  4. Recombinant Mouse Dual specificity protein phosphatase 6 (Dusp6)

Recombinant Mouse Dual specificity protein phosphatase 6 (Dusp6)

  • 货号：
    CSB-YP872042MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP872042MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP872042MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP872042MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP872042MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Dusp6; Mkp3; Dual specificity protein phosphatase 6; EC 3.1.3.16; EC 3.1.3.48; Mitogen-activated protein kinase phosphatase 3; MAP kinase phosphatase 3; MKP-3
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-381
  • 氨基酸序列
    MIDTLRPVPF ASEMAICKTV SWLNEQLELG NERLLLMDCR PQELYESSHI ESAINVAIPG IMLRRLQKGN LPVRALFTRC EDRDRFTRRC GTDTVVLYDE NSSDWNENTG GESVLGLLLK KLKDEGCRAF YLEGGFSKFQ AEFALHCETN LDGSCSSSSP PLPVLGLGGL RISSDSSSDI ESDLDRDPNS ATDSDGSPLS NSQPSFPVEI LPFLYLGCAK DSTNLDVLEE FGIKYILNVT PNLPNLFENA GEFKYKQIPI SDHWSQNLSQ FFPEAISFID EARGKNCGVL VHCLAGISRS VTVTVAYLMQ KLNLSMNDAY DIVKMKKSNI SPNFNFMGQL LDFERTLGLS SPCDNRVPTP QLYFTTPSNQ NVYQVDSLQS T
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Inactivates MAP kinases. Has a specificity for the ERK family. Plays an important role in alleviating acute postoperative pain. Necessary for the normal dephosphorylation of the long-lasting phosphorylated forms of spinal MAPK1/3 and MAP kinase p38 induced by peripheral surgery, which drives the resolution of acute postoperative allodynia. Also important for dephosphorylation of MAPK1/3 in local wound tissue, which further contributes to resolution of acute pain.
  • 基因功能参考文献：
    1. 257 genes among 55,681 genes examined were up-regulated after 30-min treatment of GT1-7 cells with GnRH. These up-regulated genes included four dual-specificity MAPK phosphatases (DUSPs), DUSP1, DUSP2, DUSP5, and DUSP6. PMID: 29899190
    2. findings suggest that DUSP6 connects TCR signaling to activation-induced metabolic commitment toward glycolysis and restrains TFH cell differentiation via inhibiting IL-21 production. PMID: 30087184
    3. mitogen-activated kinase phosphatase-3 overexpression resulted in a significant reduction of hypothalamic FoxO1 phosphorylation after insulin stimulation. PMID: 28866049
    4. DUSP6 may be a useful strategy to prevent neuronal death in neurodegenerative diseases. PMID: 28475917
    5. This study demonstrates that dusp6 deficiency is a strong genetic factor shaping gut microbiota, and that it confers obesity protection by ameliorating the gut microbiota response to diet-mediated stress. PMID: 27892926
    6. DUSP6 deficiency has limited impact on the regulation of energy metabolism, but impairs systemic glucose tolerance. PMID: 28873424
    7. Mitogen-activated protein kinase phosphatase 3 upregulation requires the activation of the Erk1/2 pathway, which correlates with the shutdown of this pathway. PMID: 26818088
    8. MKP3 could be an important factor in the regulation of brown adipocyte differentiation. PMID: 26325440
    9. exercised obese mice had a lower expression of MKP-3 and FoxO1/MKP-3 association in the liver PMID: 24396063
    10. c-Myb plays an important role in H-Ras-induced MKP-3 transcription PMID: 24978307
    11. Mice lacking MKP-3 protein develop an abnormal persistent state of mechanical allodynia following plantar incision, concurrent with long-lasting spinal phosphorylation of ERK-1/2 and p38. PMID: 24155322
    12. Depletion of DUSP6 reduced the viability of cancer cell lines and increased the cytotoxicity of EGFR and other targeted inhibitors, and cytotoxic agents. PMID: 23839489
    13. LH/hCG tightly up-regulates MKP-3 which in turn, dephosphorylates ERK1/2 and drives p21 expression. PMID: 23261984
    14. Insulin likely promotes MKP-3 protein degradation through activation of MEK/ERK pathway in liver cells. PMID: 22521266
    15. DUSP6 and PP2A participate in the regulation of melanogenesis PMID: 22544520
    16. DUSP6 is important in melanoma and it plays a different role in the distinct subtype of mouse melanoma compared with that in classic human melanoma. PMID: 22171919
    17. The reduced MKP3 expression increased the phosphorylation of Akt and Erk5, and this was correlated with an enhanced cell migration in response to PDGF-BB stimulation. PMID: 22100392
    18. ERK cascade can act as a coincidence detector to decode the simultaneous engagement of a receptor tyrosine kinase and of LRP-1 and as a signal integrator that encodes this information in a spatially and temporally distinct biological signal PMID: 21610072
    19. Results indicate that Dusp6 is a major regulator of epididymal cell proliferation in the caput and corpus regions (but not in the initial segment). PMID: 20650883
    20. mPyst1 messenger RNA is found in important signaling centers associated with cell proliferation and patterning in developing mouse embryos PMID: 11960712
    21. cloning and expression analysis during embryogenesis PMID: 12128234
    22. MKP3 mediates the cellular response to FGF8 in the mouse limb. PMID: 12766772
    23. During mouse limb bud development, Mkp3, a known MAPK/ERK regulator, is induced in the mesoderm by FGF8 signaling, through the PI3/Akt pathway. PMID: 12776124
    24. cytoplasmic localization of MKP3 is mediated by a chromosome region maintenance-1 (CRM1)-dependent nuclear export pathway; the ability of MKP3 to cause the cytoplasmic retention of ERK2 requires both a functional kinase interaction motif and NES PMID: 15269220
    25. CK2 selectively phosphorylates MKP3, suggesting cross-regulation between CK2alpha and MKP3, as well as a modulation of ERK2-MAPK signaling by CK2alpha via MKP3 PMID: 15284227
    26. MKP3 has a negative feedback action on the MAPK/ERK-mediated FGF8 pathway in the mouse neuroepithelium. PMID: 15572144
    27. Dusp6 is a negative feedback regulator of FGF-stimulated ERK signaling during mouse development. PMID: 17164422
    28. Data show that Dusp6 transcripts is expressed in partially overlapping patterns that correspond to regions of active FGF signaling, suggesting combinatorial roles in negative regulation of this pathway during ear development. PMID: 18058922
    29. In this study, association analyses were preformed in silico between the variation I62M (rs13480726) of the Dusp6 (Mkp3) gene and the mouse forebrain weight/structure in 385 mice of 20 strains. PMID: 18344456
    30. WT1 transactivates another important negative regulator of the Ras/MAPK pathway, MAPK phosphatase 3 (MKP3). PMID: 18644985
    31. The molecular basis of DUSP6 gene expression in undifferentiated state of ES cells is highly conserved during evolution. PMID: 19032937
    32. cellular microglial pathway triggered by CBR2 activation; reduction of pro-inflammatory factors and microglial migration via MKP-3 induction is part of the mechanism of action of CBR2 agonists PMID: 19476641

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Protein-tyrosine phosphatase family, Non-receptor class dual specificity subfamily
  • 数据库链接：

    KEGG: mmu:67603

    STRING: 10090.ENSMUSP00000020118

    UniGene: Mm.1791