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  4. Recombinant Mouse Dual specificity protein phosphatase 1 (Dusp1)

Recombinant Mouse Dual specificity protein phosphatase 1 (Dusp1)

  • 货号：
    CSB-YP007238MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP007238MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP007238MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP007238MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP007238MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Dusp1; 3ch134; Mkp1; Ptpn10; Ptpn16Dual specificity protein phosphatase 1; EC 3.1.3.16; EC 3.1.3.48; Mitogen-activated protein kinase phosphatase 1; MAP kinase phosphatase 1; MKP-1; Protein-tyrosine phosphatase 3CH134; Protein-tyrosine phosphatase ERP
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-367
  • 氨基酸序列
    MVMEVGILDA GGLRALLREG AAQCLLLDCR SFFAFNAGHI AGSVNVRFST IVRRRAKGAM GLEHIVPNAE LRGRLLAGAY HAVVLLDERS ASLDGAKRDG TLALAAGALC REARSTQVFF LQGGYEAFSA SCPELCSKQS TPTGLSLPLS TSVPDSAESG CSSCSTPLYD QGGPVEILSF LYLGSAYHAS RKDMLDALGI TALINVSANC PNHFEGHYQY KSIPVEDNHK ADISSWFNEA IDFIDSIKDA GGRVFVHCQA GISRSATICL AYLMRTNRVK LDEAFEFVKQ RRSIISPNFS FMGQLLQFES QVLAPHCSAE AGSPAMAVLD RGTSTTTVFN FPVSIPVHPT NSALNYLKSP ITTSPSC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle.
  • 基因功能参考文献：
    1. Low DUSP1 expression is associated with cerebral ischaemia reperfusion injury. PMID: 30138595
    2. Results indicate that the upregulation of anterior cingulate cortex (ACC, areas 24a and 24b) MKP-1 is necessary for the expression of depressive symptoms induced by chronic pain, chronic stress, and optogenetic activation of the ACC. PMID: 28359564
    3. The results suggested that DUSP1 gene silencing promoted the release of proinflammatory cytokines through activation of the MAPK signaling pathway in mice with acute pancreatitis. PMID: 29393354
    4. The cardiac ischemia/reperfusion injury - induced DUSP1 deficiency promoted the activation of JNK which upregulated the expression of the mitochondrial fission factor (Mff). PMID: 29149759
    5. Study demonstrates that MKP-1 serves as a master-regulator of macrophage phenotype and function and its dysregulation by metabolic stress may be a major contributor to atherogenesis and the progression of atherosclerotic plaques. PMID: 27670844
    6. FAM3D inhibits glucagon secretion via MKP1-dependent suppression of ERK1/2 signaling. PMID: 28247283
    7. these data show that MKP-1 is an important endogenous suppressor of innate immune responses involved in the regulation of blood-testis barrier barrier dynamic PMID: 27783995
    8. MKP-1 regulates IL-1beta production through stabilization of HIF-1alpha. The induction of HIF-1alpha protein in MKP-1 deficiency is partly due to p38MAPK activation in response to LPS. PMID: 28238855
    9. this work identifies a previously unrecognized role for DUSP1 in regulating autophagy. PMID: 27459239
    10. c-FOS and DUSP1 expression levels determine the threshold of tyrosine kinase inhibitor (TKI) efficacy, such that growth-factor-induced expression of c-FOS and DUSP1 confers intrinsic resistance to TKI therapy in a wide-ranging set of leukemias. PMID: 28319094
    11. PTHrP counteracts the pro-apoptotic actions of reactive oxygen species by a mechanism dependent on MKP1-induced dephosphorylation. PMID: 27357344
    12. A2AR signaling regulates both basal and LPS-induced DUSP1 levels in macrophages via activating the adenylate cyclase pathway. PMID: 27066978
    13. these data suggest an important role for DUSPs in regulating MAPK dephosphorylation, with an emphasis on DUSP1, during early adipogenesis PMID: 26566083
    14. Loss of DUSP1 does not cause changes in cartilage degeneration and gait in a mouse model of spontaneous osteoarthritis at 21 months of age. PMID: 26562438
    15. Nr4a1 induction is dependent on ERK1/2 and that MKP-1 negatively regulates this induction. PMID: 25657047
    16. MKP-1 negatively regulates chemokine-driven osteoclast formation and subsequent bone resorption in response to LPS stimulation PMID: 25261746
    17. DUSP1 and tristetraprolin cooperate to regulate macrophage responses to lipopolysaccharide. PMID: 26019272
    18. MKP-1 regulated the expression of MMP-12. PMID: 25310910
    19. CYLD negatively regulates nontypeable Haemophilus influenzae-induced IL-8 expression via MKP-1-dependent inhibition of ERK. PMID: 25389768
    20. Ozone exposure decreased the protein expression of MKP-1 in an asthma model. PMID: 25287866
    21. hepatic MKP-1 serves as a selective regulator of MAPK-dependent signals that contributes to the maintenance of glucose homeostasis and peripheral tissue energy balance PMID: 25312648
    22. MKP-1 and MKP-2 stability is regulated by ERK-mediated phosphorylation through a degradation pathway independent of polyubiquitination PMID: 25204653
    23. MKP-1 negatively regulates MAPK signaling in the mouse hypothalamus. PMID: 24686178
    24. The results of this study imply that MKP-1 possesses anti-apoptotic and anti-inflammatory properties by simultaneously controlling the activities of JNK and microglial p38. PMID: 24842488
    25. DUSP1 is actively involved in the antiviral host defense mechanism against a poxvirus infection. PMID: 24244156
    26. MKP-1 promoted angiogenic and arteriogenic neovascular growth, potentially through dephosphorylation of histone H3 serine 10 on coding-region DNA to control transcription of angiogenic genes, such as fractalkine. PMID: 24578378
    27. results show that HDAC1, -2, and -3 deacetylate MKP-1 and that this post-translational modification increases MAPK signaling and innate immune signaling PMID: 24374966
    28. rapamycin treatment led to an increased MKP1 expression in BMDM from WT but failed to do so in BMDMs lacking the AKT1 isoform or MEK1 and MEK2. PMID: 24126911
    29. Mitogen-activated protein kinase phosphatase (Mkp)-1 protects mice against acetaminophen-induced hepatic injury. PMID: 22623522
    30. MKP-1 provides neuroprotection in a mouse model of Huntington's disease by inhibiting p38 and JNK kinase. PMID: 23392662
    31. Dusp1 and Dusp4 are cardioprotective genes that play a critical role in the heart by dampening p38 MAPK signaling that would otherwise reduce contractility and induce cardiomyopathy. PMID: 22993413
    32. Notch3 inhibits the myogenic transcription factor Mef2c by increasing the activity of Mkp1. PMID: 23055528
    33. MKP-1 regulates RANKL gene expression and is essential for vitamin D signaling. PMID: 22899855
    34. A sexual dimorphism exists in the periapical inflammatory process, where male mkp-1(-/-) mice have more inflammation than female mkp-1(-/-) mice. The increase in inflammatory infiltrate correlates to more bone loss in the male mice. PMID: 22794213
    35. The MKP-1 activity is required to exclude C/EBPbeta from the nucleus basally, and that MKP-1 antagonizes C/EBPbeta expression after global forebrain ischemia, particularly within the vulnerable CA1 sector of the hippocampus. PMID: 22522596
    36. MKP-1 suppressed Tristetraprolin expression by inhibiting p38 MAPK pathway. PMID: 23030431
    37. Data indicate that knockdown of MKP-1 mimicked the priming effects of metabolic stress. PMID: 22991462
    38. MKP-1 is regulated the evolution as well as the resolution of inflammation during muscle stem cell-dependent tissue repair by controlling p38-mediated AKT activation. PMID: 22361726
    39. mapracorat exerts its anti-inflammatory effects, at least in part, by augmenting MKP-1 expression PMID: 22898817
    40. Data revealed that ZFP36L1 and ZFP36L2 bound to the 3' untranslated region (UTR) of MAPK phosphatase-1 (MKP-1) mRNA and downregulated Mkp-1 3'UTR-mediated luciferase activity. PMID: 22701344
    41. nocodazole increases the ERK activity to enhance Dusp1(MKP-1) expression which inhibits p38 activation induced by TNF-alpha. PMID: 22294037
    42. DUSP-1 is a critical regulator of osteoclast activity and limits bone destruction in an experimental model of rheumatoid arthritis PMID: 22275313
    43. GR dimerization inhibits JNK2 through MKP1 and protects from TNF-induced apoptosis and lethal inflammation. PMID: 22585571
    44. The results support a pivotal role of Mkp-1 as a negative regulator of mucosal immune responses and highlight its protective function against inflammatory bowel disease. PMID: 22461024
    45. MKP-1, one of immediate early genes in stress conditions, was induced at transcriptional level in hypoxia/re-oxygenation in neuroblastoma N1E115 cells. PMID: 22197701
    46. MKP1 represents an important negative feedback mechanism limiting pro-inflammatory response in the heart during sepsis. PMID: 22198506
    47. Upregulation of MKP-1 by vitamin D is a novel pathway by which vitamin D inhibits lipopolysaccharide-induced p38 MAP kinase activation and cytokine production in monocytes/macrophages. PMID: 22301548
    48. Data shows MKP-1 plays an important role in the control of macrophage activation by the adaptive immune system. PMID: 21956667
    49. novel regulatory mechanism involving the appropriate balance of p38, MKP-1, miR-21, and AKT activities may have implications in chronic inflammatory degenerative diseases. PMID: 21987635
    50. TSA upregulates the expression of C/EBP-beta and MKP-1, which may downregulate pro-osteoclastogenic factors and signaling molecules, ultimately suppressing osteoclastogenesis. PMID: 22172035

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Protein-tyrosine phosphatase family, Non-receptor class dual specificity subfamily
  • 数据库链接：

    KEGG: mmu:19252

    STRING: 10090.ENSMUSP00000025025

    UniGene: Mm.239041