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  4. Recombinant Mouse Dual specificity protein kinase CLK2 (Clk2)

Recombinant Mouse Dual specificity protein kinase CLK2 (Clk2)

  • 货号：
    CSB-YP005558MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP005558MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP005558MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP005558MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP005558MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Clk2
  • Uniprot No.：
  • 别名：
    Clk2Dual specificity protein kinase CLK2; EC 2.7.12.1; CDC-like kinase 2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-499
  • 氨基酸序列
    MPHPRRYHSS ERGSRGSYHE HYQSRKHKRR RSRSWSSSSD RTRRRRREDS YHVRSRSSYD DHSSDRRLYD RRYCGSYRRN DYSRDRGEAY YDTDFRQSYE YHRENSSYRS QRSSRRKHRR RRRRSRTFSR SSSHSSRRAK SVEDDAEGHL IYHVGDWLQE RYEIVSTLGE GTFGRVVQCV DHRRGGTQVA LKIIKNVEKY KEAARLEINV LEKINEKDPD NKNLCVQMFD WFDYHGHMCI SFELLGLSTF DFLKDNNYLP YPIHQVRHMA FQLCQAVKFL HDNKLTHTDL KPENILFVNS DYELTYNLEK KRDERSVKST AVRVVDFGSA TFDHEHHSTI VSTRHYRAPE VILELGWSQP CDVWSIGCII FEYYVGFTLF QTHDNREHLA MMERILGPVP SRMIRKTRKQ KYFYRGRLDW DENTSAGRYV RENCKPLRRY LTSEAEDHHQ LFDLIENMLE YEPAKRLTLG EALQHPFFAC LRTEPPNTKL WDSSRDISR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing and can cause redistribution of SR proteins from speckles to a diffuse nucleoplasmic distribution. Acts as a suppressor of hepatic gluconeogenesis and glucose output by repressing PPARGC1A transcriptional activity on gluconeogenic genes via its phosphorylation. Phosphorylates PPP2R5B thereby stimulating the assembly of PP2A phosphatase with the PPP2R5B-AKT1 complex leading to dephosphorylation of AKT1. Phosphorylates: PTPN1, SRSF1 and SRSF3. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Phosphorylates PAGE4 at several serine and threonine residues and this phosphorylation attenuates the ability of PAGE4 to potentiate the transcriptional activator activity of JUN.
  • 基因功能参考文献：
    1. CLK2 is a regulatory component of diet-induced thermogenesis in brown adipose tissue. PMID: 28089567
    2. Liver-specific Clk2 knockout mice fed a high-fat diet exhibit increased fasting levels of blood ketone bodies, reduced respiratory exchange ratio, and increased gene expression of fatty acid oxidation and ketogenic pathways. PMID: 24458359
    3. LAMMER/Cdc2-like kinase, represented by Doa and its mammalian homolog CLK2, is a critical and conserved component in the regulatory program of the mitosis-to-meiosis switch PMID: 23376537
    4. Cdc2-like kinase 2 (Clk2) directly phosphorylates the SR domain on PGC-1alpha, resulting in repression of gluconeogenic gene expression and hepatic glucose output. PMID: 20074525
  • 亚细胞定位：
    Nucleus. Nucleus speckle.
  • 蛋白家族：
    Protein kinase superfamily, CMGC Ser/Thr protein kinase family, Lammer subfamily
  • 数据库链接：

    KEGG: mmu:12748

    STRING: 10090.ENSMUSP00000113390

    UniGene: Mm.288098