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  4. Recombinant Mouse Dual specificity mitogen-activated protein kinase kinase 7 (Map2k7)

Recombinant Mouse Dual specificity mitogen-activated protein kinase kinase 7 (Map2k7)

  • 货号：
    CSB-YP807005MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP807005MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP807005MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP807005MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP807005MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Map2k7; Mkk7; Dual specificity mitogen-activated protein kinase kinase 7; MAP kinase kinase 7; MAPKK 7; EC 2.7.12.2; JNK-activating kinase 2; MAPK/ERK kinase 7; MEK 7; c-Jun N-terminal kinase kinase 2; JNK kinase 2; JNKK 2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-535
  • 氨基酸序列
    AASSLEQKL SRLEAKLKQE NREARRRIDL NLDISPQRPR PIIVITLSPA PAPSQRAALQ LPLANDGGSR SPSSESSPQH PTPPTRPRHM LGLPSTLFTP RSMESIEIDQ KLQEIMKQTG YLTIGGQRYQ AEINDLENLG EMGSGTCGQV WKMRFRKTGH IIAVKQMRRS GNKEENKRIL MDLDVVLKSH DCPYIVQCFG TFITNTDVFI AMELMGTCAE KLKKRMQGPI PERILGKMTV AIVKALYYLK EKHGVIHRDV KPSNILLDER GQIKLCDFGI SGRLVDSKAK TRSAGCAAYM APERIDPPDP TKPDYDIRAD VWSLGISLVE LATGQFPYKN CKTDFEVLTK VLQEEPPLLP GHMGFSGDFQ SFVKDCLTKD HRKRPKYNKL LEHSFIKHYE ILEVDVASWF KDVMAKTESP RTSGVLSQHH LPFFSTSVTW GAWPLAAQTP FQSGVIRCRG RVPSPRRATG GSGGQPCVCA GGPGPSFTEM GPSPSPMLSN TFFTPDPGAC PGASTWGLPR RRLCQLLTTS TPGCC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Has a specific role in JNK signal transduction pathway activated by proinflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Part of a non-canonical MAPK signaling pathway, composed of the upstream MAP3K12 kinase and downstream MAP kinases MAPK1/ERK2 and MAPK3/ERK1, that enhances the AP-1-mediated transcription of APP in response to APOE.
  • 基因功能参考文献：
    1. High Map2k7 expression is associated with T-cell acute lymphoblastic leukemia. PMID: 27872496
    2. Arrestin-3 directly interacts with MKK7 and promotes JNK3alpha2 phosphorylation by both MKK4 and MKK7 in vitro as well as in intact cells. PMID: 23960075
    3. MKK7 is specifically phosphorylated in the neurite shaft which triggers Map1b phosphorylation to regulate microtubule bundling leading to neurite elongation. PMID: 23226105
    4. Data indicate that mitogen activated protein kinase kinase 7 (MKK7) plays a critical regulatory role in the c-Jun N-terminal kinase pathway in a murine model of rheumatoid arthritis. PMID: 22353730
    5. a novel function for the stress kinase MKK7 as a regulator of the circadian clock in mammalian cells at steady state. PMID: 22267733
    6. This study demonistrated that Stress-activated protein kinase MKK7 regulates axon elongation in the developing cerebral cortex. PMID: 22090513
    7. The results reveal an essential role of MKK7 in cardiomyocytes for protecting the heart from hypertrophic insults thereby preventing the transition to heart failure. PMID: 21284947
    8. The role of NF-kappaB and c-jun N-terminal kinase (JNK) activation in RANKL-induced osteoclast differentiation was investigated using an adenovirus vector carrying the dominant negative 1kappaB kinase 2 gene or dominant negative MKK7 gene. PMID: 11918218
    9. myocardial MKK7D has a role in significant JNK activation, robust induction of the fetal gene program, and contractile dysfunction PMID: 14742426
    10. Genetic inactivation of the stress signalling kinase, MKK7, a direct activator of JNKs in mice, results in embryonic lethality and impaired proliferation of hepatocytes. PMID: 15039780
    11. Changes in MKK7 gene expression between subspecies of Mus musculus are most likely affected by positive selection. PMID: 16910979
    12. A noncanonical function of Map2k5 and Map3k2 domain is decribed for coordinating erk5 kinase and map2k7 signaling. PMID: 17452462

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase subfamily
  • 组织特异性：
    Expressed at high levels in brain, lung, liver, skeletal muscle, kidney, and testis and at lower levels in the heart and spleen.
  • 数据库链接：

    KEGG: mmu:26400

    STRING: 10090.ENSMUSP00000003027

    UniGene: Mm.3906