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  4. Recombinant Mouse Double-strand-break repair protein rad21 homolog (Rad21)

Recombinant Mouse Double-strand-break repair protein rad21 homolog (Rad21)

  • 货号：
    CSB-YP713978MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP713978MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP713978MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP713978MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP713978MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Rad21; Hr21; Scc1; Double-strand-break repair protein rad21 homolog; mHR21; Pokeweed agglutinin-binding protein 29; PW29; SCC1 homolog) [Cleaved into: 64-kDa C-terminal product; 64-kDa carboxy-terminal product)]
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-635
  • 氨基酸序列
    MFYAHFVLSK RGPLAKIWLA AHWDKKLTKA HVFECNLESS VESIISPKVK MALRTSGHLL LGVVRIYHRK AKYLLADCNE AFIKIKMAFR PGVVDLPEEN REAAYNAITL PEEFHDFDQP LPDLDDIDVA QQFSLNQSRV EEITMREEVG NISILQENDF GDFGMDDREI MREGSAFEDD DMLVSTSASN LLLEPEQSTS NLNEKMNHLE YEDQYKDDNF GEGNDGGILD DKLISNNDGG IFDDPPALSE AGVMLPEQPA HDDMDEDDNG SLGGPDSPDS VDPVEPMPTM TDQTTLVPNE EEAFALEPID ITVKETKAKR KRKLIVDSVK ELDSKTIRAQ LSDYSDIVTT LDLAPPTKKL MMWKETGGVE KLFFLPAQPL WNNRLLKLFT RCLTPLVPED LRKRRKGGEA DNLDEFLKEF ENPEVPREEQ QPQQQQPQPQ RDVIDEPIIE EPSRLQDSVM EASRTTIEES AMPPPPPQGV KRKAGQIDPE PSIPPQQVEQ MEIPPVELPP EEPPNICQLI PELELLPEKE KEKEKEKEEE EEEEDEDASG GDQDQEERRW NKRTQQMLHG LQRALAKTGA ESISLLELCR NTNRKQAAAK FYSFLVLKKQ QAIELTQEEP YSDIIATPGP RFHII
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    As a member of the cohesin complex, involved in sister chromatid cohesion from the time of DNA replication in S phase to their segregation in mitosis, a function that is essential for proper chromosome segregation, post-replicative DNA repair, and the prevention of inappropriate recombination between repetitive regions. The cohesin complex may also play a role in spindle pole assembly during mitosis. In interphase, cohesins may function in the control of gene expression by binding to numerous sites within the genome. May control RUNX1 gene expression. Binds to and represses APOB gene promoter. May play a role in embryonic gut development, possibly through the regulation of enteric neuron development.; May promote apoptosis.
  • 基因功能参考文献：
    1. The results demonstrate that the zygotic genome folds into loops and domains that critically depend on Scc1-cohesin and that are regulated in size and linear density by Wapl. PMID: 29217590
    2. n keratinocytes, the promoter-enhancer anchoring regions in the gene-rich transcriptionally active TADs are enriched for the binding of chromatin architectural proteins CTCF, Rad21 and chromatin remodeler Brg1. In contrast to gene-rich TADs, gene-poor TADs show preferential spatial contacts with each other, do not contain active enhancers and show decreased binding of CTCF, Rad21 and Brg1 in keratinocytes PMID: 28863138
    3. the cohesin complex regulates PRC2 targeting to silence Hoxa7 and Hoxa9 and negatively regulate self-renewal. Our studies identify a novel epigenetic mechanism underlying leukemogenesis in AML patients with cohesin mutations. PMID: 27554164
    4. Knockdown of Rad21 reduces expression of the constitutive, biallelically expressed Pcdhalpha isoforms alphac1 and alphac2. PMID: 22550178
    5. show that spermatocytes from mice lacking the two meiosis-specific cohesin subunits RAD21L and REC8 were unable to initiate RAD51- but not DMC1-mediated double-strand break repair PMID: 22711701
    6. findings firmly establish a cell-division-independent role for cohesin in Tcra locus rearrangement and provide a comprehensive account of the mechanisms by which cohesin enables cellular differentiation PMID: 21832993
    7. knock-down of RAD21 causes expression changes that are similar to expression changes after Nanog depletion, demonstrating the functional relevance of the RAD21--pluripotency transcriptional network association PMID: 21589869
    8. The signal of RAD21 was prominent in somatic cells outside the seminiferous tubules or in spermatogonia, and was also detected in some spermatocytes localizing away from the basement membrane. PMID: 21242291
    9. Rad21 is a DNA-damage response gene that markedly affects animal and cell survival PMID: 20711430
    10. a cohesin complex containing Rad21 and STAG2 cooperates with a STAG3-specific complex to maintain sister chromatid cohesion during the diplotene stage of meiosis PMID: 12034751
    11. Analyses of mRNA expression patterns of cohesin subunits Rad21 and Rec8 in mice: germ cell-specific expression of rec8 mRNA in both male and female mice. PMID: 12130806
    12. Rad21, and the superimposed SCP3 and SCP2, are involved in the monopolar attachment of sister kinetochores during meiosis I PMID: 14970259

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  • 亚细胞定位：
    [Double-strand-break repair protein rad21 homolog]: Nucleus. Chromosome. Chromosome, centromere.; [64-kDa C-terminal product]: Cytoplasm, cytosol. Nucleus.
  • 蛋白家族：
    Rad21 family
  • 组织特异性：
    Widely expressed with highest levels in testis, brain, kidney, heart and thymus. Lowest levels in skeletal muscle.
  • 数据库链接：

    KEGG: mmu:19357

    STRING: 10090.ENSMUSP00000022927

    UniGene: Mm.182628