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  4. Recombinant Mouse Docking protein 3 (Dok3)

Recombinant Mouse Docking protein 3 (Dok3)

  • 货号：
    CSB-YP007109MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP007109MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP007109MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP007109MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP007109MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Dok3
  • Uniprot No.：
  • 别名：
    Dok3; DoklDocking protein 3; Downstream of tyrosine kinase 3; p62(dok)-like protein; DOK-L
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-444
  • 氨基酸序列
    MESVEPPVKD GILYQQHVKF GKKCWRKVWA LLYAGGPSGV ARLESWDVRD GGLGPAGDRS TGPSRRGERR VIRLADCVSV LPADGESCPR DTGAFLITTT ERSHLLAAQH RQSWVDPICQ LAFPGTGECS SGSGQAESPK RGFVPMEENS IYSSWQEVTE FPVIVQRTEA TSRCQLKGPY LLVLGQDDIQ LRETSKPQAC FSWPYRFLRK YGSDKGVFSF EAGRRCDSGE GLFAFSSPRA PDICGVVAAA IARQRERLPE LAMSPPCPLP RALSLPSLEP PGELREVAPG FELPTPRKLP LTDPGPQSLP LLLSPTQEGP ASGLYASVCK QTSKHTGTAE HLYENVCMLE ASPGLTNGGP EAQEGPPGGR SPLGSPIYHN TEDLSWPGSA QDSNLEAQYR RLLELELDEA GSAGRSGAQA GIKAKLVTLL TRERKKGPAP CDRP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate ABL1 function.
  • 基因功能参考文献：
    1. DOK3 regulates bone remodeling by negatively regulating M-CSF- and RANKL-mediated osteoclastogenesis and positively regulating osteoblastogenesis. PMID: 28650106
    2. findings demonstrate that Dok-3 and Dok-1/-2 play distinctive but cooperative roles in osteoclastogenesis and protect mice from osteopenia, providing physiological and pathophysiological insight into bone homeostasis. PMID: 29548825
    3. DOK3 lung expression negatively regulates the development of lipopolysaccharide-induced acute respiratory distress syndrome in mice. PMID: 28590889
    4. This study reveals DOK3 as a nonredundant regulator of plasma cell differentiation by up-regulating PD-1 ligand expression through the attenuation of calcium signaling. PMID: 25053811
    5. Triple Dok1 Doc2 Doc3 knockout leads to spontaneous pulmonary inflammation with hallmarks of asthma. PMID: 23205702
    6. absence of DOK3 increases LPS signaling, contributing to LPS-induced tolerance. Thus, DOK3 plays a role in TLR signaling during both naive and endotoxin-induced tolerant conditions PMID: 22761938
    7. identify a surprising but pivotal role for dynein and the microtubule network alongside Grb2, Dok-3, and Cbl in antigen gathering during B cell activation PMID: 21703542
    8. Subcellular localization of Grb2 by the adaptor protein Dok-3 restricts the intensity of Ca2+ signaling in B cells. PMID: 17290227
    9. In the absence of Dok-3, the localization of the inhibitory phosphatase SHIP-1 to the plasma membrane is intact while its phosphorylation is compromised, suggesting that Dok-3 could function to facilitate or sustain the activation of SHIP-1. PMID: 17363732

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  • 亚细胞定位：
    Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
  • 蛋白家族：
    DOK family, Type A subfamily
  • 组织特异性：
    Predominantly expressed in bone marrow, spleen and lung. Low levels in heart, brain, liver, muscle, thymus, kidney and testis. Highly expressed in B-cells and macrophages.
  • 数据库链接：

    KEGG: mmu:27261

    STRING: 10090.ENSMUSP00000046695

    UniGene: Mm.33910