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  4. Recombinant Mouse Disabled homolog 2 (Dab2)

Recombinant Mouse Disabled homolog 2 (Dab2)

  • 货号：
    CSB-YP006480MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP006480MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP006480MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP006480MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP006480MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Dab2; Doc2; Disabled homolog 2; Adaptor molecule disabled-2; Differentially expressed in ovarian carcinoma 2; DOC-2; Mitogen-responsive phosphoprotein
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-766
  • 氨基酸序列
    SNEVETSTT NGQPDQQAAP KAPSKKEKKK GSEKTDEYLL ARFKGDGVKY KAKLIGIDDV PDARGDKMSQ DSMMKLKGMA AAGRSQGQHK QRIWVNISLS GIKIIDEKTG VIEHEHPVNK ISFIARDVTD NRAFGYVCGG EGQHQFFAIK TGQQAEPLVV DLKDLFQVIY NVKKKEEDKK KVEEANKAEE NGSEALMTLD DQANKLKLGV DQMDLFGDMS TPPDLNSPTE SKDILLVDLN SEIDTNQNSL RENPFLTNGV TSCSLPRPKP QASFLPENAF SANLNFFPTP NPDPFRDDPF AQPDQSAPSS FDSLTSPDQK KASLSSSSTP QSKGPLNGDT DYFGQQFDQL SNRTGKPEAQ GGPWPYPSSQ TQQAVRTQNG VSEREQNGFH IKSSPNPFVG SPPKGLSVPN GVKQDLESSV QSSAHDSIAI IPPPQSTKPG RGRRTAKSSA NDLLASDIFA SEPPGQMSPT GQPAVPQSNF LDLFKGNAPA PVGPLVGLGT VPVTPPQAGP WTPVVYSPST TVVPGAIISG QPPSFRQPLV FGTTPAVQVW NQSPSFATPA SPPPPTVWCP TTSVAPNAWS STSPLGNPFQ SNNIFPPPTM STQSSPQPMM SSVLATPPQP PPRNGPLKDI PSDAFTGLDP LGDKEVKEVK EMFKDFQLRQ PPLVPSRKGE TPPSGTSSAF SSYFNNKVGI PQEHVDHDDF DANQLLNKIN EPPKPAPRQG VLLGTKSADN SLENPFSKGF SSSNPSVVSQ PASSDPHRSP FGNPFA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Adapter protein that functions as clathrin-associated sorting protein (CLASP) required for clathrin-mediated endocytosis of selected cargo proteins. Can bind and assemble clathrin, and binds simultaneously to phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and cargos containing non-phosphorylated NPXY internalization motifs, such as the LDL receptor, to recruit them to clathrin-coated pits. Can function in clathrin-mediated endocytosis independently of the AP-2 complex. Involved in endocytosis of integrin beta-1; this function seems to redundant with the AP-2 complex and seems to require DAB2 binding to endocytosis accessory EH domain-containing proteins such as EPS15, EPS15L1 and ITSN1. Involved in endocytosis of cystic fibrosis transmembrane conductance regulator/CFTR. Isoform p96 is involved in endocytosis of megalin/LRP2 lipoprotein receptor during embryonal development. Required for recycling of the TGF-beta receptor. Isoform p67 is not involved in LDL receptor endocytosis. Involved in CFTR trafficking to the late endosome. Involved in several receptor-mediated signaling pathways. Involved in TGF-beta receptor signaling and facilitates phosphorylation of the signal transducer SMAD2. Mediates TFG-beta-stimulated JNK activation. May inhibit the canoniocal Wnt/beta-catenin signaling pathway by stabilizing the beta-catenin destruction complex through a competing association with axin preventing its dephosphorylation through protein phosphatase 1 (PP1). Sequesters LRP6 towards clathrin-mediated endocytosis, leading to inhibition of Wnt/beta-catenin signaling. May activate non-canonical Wnt signaling. In cell surface growth factor/Ras signaling pathways proposed to inhibit ERK activation by interrupting the binding of GRB2 to SOS1 and to inhibit SRC by preventing its activating phosphorylation at 'Tyr-419'. Proposed to be involved in modulation of androgen receptor (AR) signaling mediated by SRC activation; seems to compete with AR for interaction with SRC. Plays a role in the CSF-1 signal transduction pathway. Plays a role in cellular differentiation. Involved in cell positioning and formation of visceral endoderm (VE) during embryogenesis and proposed to be required in the VE to respond to Nodal signaling coming from the epiblast. Required for the epithelial to mesenchymal transition, a process necessary for proper embryonic development. May be involved in myeloid cell differentiation and can induce macrophage adhesion and spreading. Isoform p67 may be involved in transcriptional regulation. May act as a tumor suppressor.
  • 基因功能参考文献：
    1. miR149 was able to target Dab2 and promote the cardiac differentiation of mouse MSCs in vitro, which depended upon the Wnt/betacatenin signaling pathway. PMID: 29693140
    2. Dab2 functions as a negative immune regulator of TLR4 endocytosis and signaling; it has a role in the regulatory circuit in controlling the inflammatory response of macrophages to endotoxin PMID: 27748405
    3. The results suggest that Dab2 is required for the excessive calorie-induced differentiation of an adipocyte progenitor cell population that is present in juvenile but depleted in mature animals. The finding provides evidence for a limited pre-adipocyte population in juvenile mammals and the requirement of Dab2 in the regulation of Ras/MAPK signal in the commitment of the precursor cells to adipose tissues. PMID: 27779214
    4. The various novel mechanisms are described by which Dab2 mediates an array of signaling events with vast physiological consequences. PMID: 27417122
    5. The combination of Arh and Dab2 is responsible for the majority of adaptor function in LDLR endocytosis and LDLR-mediated cholesterol homeostasis. PMID: 27005486
    6. Results identified a novel activation marker, DAB2, which expression is significantly different between microglia activation stages M2a and either M1 or M2b. PMID: 26424600
    7. these findings reveal that DAB2 is critical for controlling inflammatory signaling during phenotypic polarization of macrophages PMID: 26927671
    8. Dab2 expression is induced by hormones PMID: 25360623
    9. Results suggest that endogenous Dab2 exacerbates central nervous system inflammation, potentially acting to up-regulate reactive oxygen species expression in macrophages and microglia, and that it is of potential pathogenic relevance in multiple sclerosis PMID: 24252604
    10. Disabled-2 is required for efficient hemostasis and platelet activation by thrombin in mice. PMID: 25212232
    11. Akt1 and Akt2 are involved in albumin endocytosis, and phosphorylation of Dab2 by Akt induces albumin endocytosis in proximal tubule epithelial cells. PMID: 25253241
    12. Study of dab2 mutant allele in embryos and embryoid bodies confirms a role for Dab2 in extraembryonic endoderm development and epithelial organization. Also, Dab2 has a physiological role in the endocytosis of lipoproteins and cholesterol metabolism. PMID: 24168030
    13. miR-145 regulates the migration and invasion of highly invasive prostate cancer cells through targeting DAB2 gene PMID: 24846918
    14. these results indicate that p96 Dab2 plays a key role in the extra-embryonic endodermal differentiation process. PMID: 22525672
    15. The s report that Dab2, previously described as an inhibitor of Wnt/beta-catenin signalling, selectively recruits LRP6 to the clathrin-dependent endocytic route, thereby sequestering it from caveolin-mediated endocytosis. PMID: 22491013
    16. Data show that although small RNA-mediated Dab2 knockdown had no affect on the internalization of various clathrin-dependent cargo, TGF-beta receptor recycling was abrogated. PMID: 20881059
    17. TGF-beta modulates the expression of Dab2 and ILEI mRNA required for epithelial-mesenchymal transdifferentiation by inducing phosphorylation of hnRNP E1, resulting in its release from a structural, 33-nucleotide BAT element in the 3'UTR of Dab2 and ILEI. PMID: 20154680
    18. Dab2 is pleiotropic and regulates both visceral endoderm function and lipoprotein receptor trafficking in vivo. PMID: 11927540
    19. Data suggest that Dab2 is a member of a family of cargo-specific adaptor proteins, which regulate clathrin-coat assembly at the plasma membrane. PMID: 12234931
    20. is essential for endodermal cell positioning and structure formation during mouse embryogenesis PMID: 12413896
    21. Dab2 plays an important regulatory role during transforming growth factor beta-induced epithelial to mesenchymal transition PMID: 15734730
    22. Dab2 is localized in the apical endocytic apparatus of the renal proximal tubule and that this localization requires megalin PMID: 15870384
    23. These studies suggest that Dab2 p96 mediates endocytosis of megalin in the VE. In addition, rescue of embryonic viability correlates with endocytosis, suggesting that endocytosis mediated by Dab2 is important for normal development. PMID: 16263760
    24. Dab2 gene was cloned and its regulatory region in the prostate was characterized. PMID: 16629596
    25. This study defines a role of DAB2 in the control of neuronal plasticity and demonstrates for the first time that DAB2 is a negative regulator in nerve growth factor-mediated neurite outgrowth. PMID: 17317100
    26. Dab2 is a surface positioning gene and suggest a novel mechanism of epithelial cell surface targeting. PMID: 17339320
    27. Decreased DOC-2/DAB2 expression is associated with urothelial carcinoma of the bladder PMID: 17671122
    28. Dab2 is dynamically expressed in various regions of the mouse central nervous system during embryonic development. PMID: 18680569
    29. Dab2 plays a role in epithelial organization by suppression of c-Fos expression PMID: 19191218
    30. One impaired function in Dab2-deficient regulatory T cells involves gap junction-mediated intercellular communication, whereas other mechanisms of Treg cell-mediated suppression examined appear largely intact. PMID: 19767570

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  • 亚细胞定位：
    Cytoplasmic vesicle, clathrin-coated vesicle membrane. Membrane, clathrin-coated pit.; [Isoform p96]: Membrane, clathrin-coated pit. Note=Colocalizes with LDLR at clathrin-coated pits.; [Isoform p67]: Cytoplasm. Nucleus. Note=Diffuse localization in the cytoplasm; does not localize to clathrin-coated pits.
  • 组织特异性：
    Isoform p96 and isoform p67 are expressed in adult kidney and fibroblasts with isoform p96 being the predominant form. Isoform p67 is the predominant isoform expressed in embryonic visceral endoderm.
  • 数据库链接：

    KEGG: mmu:13132

    STRING: 10090.ENSMUSP00000079689

    UniGene: Mm.240830