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  4. Recombinant Mouse Denticleless protein homolog (Dtl)

Recombinant Mouse Denticleless protein homolog (Dtl)

  • 货号：
    CSB-YP666228MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP666228MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP666228MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP666228MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP666228MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Dtl
  • Uniprot No.：
  • 别名：
    Dtl; Cdt2; L2dtl; RampDenticleless protein homolog; Lethal(2) denticleless protein homolog; Meth A retinoic acid-regulated nuclear matrix-associated protein; Meth A RAMP; Retinoic acid-regulated nuclear matrix-associated protein
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-729
  • 氨基酸序列
    MLFNSVLRQP QLGVLRNGWS SHYPLQSLLS GYQCNCNDEH TSYGETGVPV PPFGCTFCTA PSMEHILAVA NEEGFVRLYN TESQTSKKTC FKEWMAHWNA VFDLAWVPGE LKLVTAAGDQ TAKFWDVRAG ELMGTCKGHQ CSLKSVAFPK FQKAVFSTGG RDGNIMIWDT RCNKKDGFYR QVNQISGAHN TADKQTPSKP KKKQNSKGLA PAVDSQQSVT VVLFQDENTL VSAGAVDGII KVWDLRKNYT AYRQEPIASK SFLYPGTSTR KLGYSSLVLD STGSTLFANC TDDNIYMFNM TGLKTSPVAV FNGHQNSTFY VKSSLSPDDQ FLISGSSDEA AYIWKVSMPW HPPTVLLGHS QEVTSVCWCP SDFTKIATCS DDNTLKIWRL NRGLEEKPGD KHSIVGWTSQ KKKEVKACPV TVPSSQSTPA KAPRAKSSPS ISSPSSAACT PSCAGDLPLP SSTPTFSVKT TPATTRSSVS RRGSISSVSP KPLSSFKMSL RNWVTRTPSS SPPVTPPASE TKISSPRKAL IPVSQKSSQA DACSESRNRV KRRLDSSCLE SVKQKCVKSC NCVTELDGQA ESLRLDLCCL SGTQEVLSQD SEGPTKSSKT EGAGTSISEP PSPVSPYASE GCGPLPLPLR PCGEGSEMVG KENSSPENKN WLLAIAAKRK AENSSPRSPS SQTPSSRRQS GKTSPGPVTI TPSSMRKICT YFRRKTQDDF CSPEHSTEL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1, CDKN1A/p21(CIP1), FBH1, KMT5A and SDE2. CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. KMT5A degradation is also important for a proper regulation of mechanisms such as TGF-beta signaling, cell cycle progression, DNA repair and cell migration. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis. The DDB1-CUL4A-DTL E3 ligase complex regulates the circadian clock function by mediating the ubiquitination and degradation of CRY1.
  • 基因功能参考文献：
    1. Maternal DCAF2 protein is crucial for prevention of DNA re-replication in the first and unique mitotic cell cycle of the zygote. PMID: 28818995
    2. while interaction with PCNA was important for targeting p21 to the CRL4Cdt2 ligase re-localized to MVM replication centers PMID: 24699724
    3. L2dtl gene expression is essential for very early mouse embryonic development PMID: 17107960
  • 亚细胞定位：
    Nucleus. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome.
  • 蛋白家族：
    WD repeat cdt2 family
  • 数据库链接：

    KEGG: mmu:76843

    STRING: 10090.ENSMUSP00000027933

    UniGene: Mm.189102