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  4. Recombinant Mouse Delta-like protein 1 (Dll1), partial

Recombinant Mouse Delta-like protein 1 (Dll1), partial

  • 货号：
    CSB-YP736821MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP736821MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP736821MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP736821MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP736821MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Dll1Delta-like protein 1; Drosophila Delta homolog 1; Delta1) [Cleaved into: Dll1-soluble form; Dll1-EC; Shed form); Dll1-derived cell-associated form; Dll1-TMIC; Membrane-associated fragment); Dll1-intracellular form; Dll1-IC)]
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transmembrane ligand protein of NOTCH1, NOTCH2 and NOTCH3 receptors that binds the extracellular domain (ECD) of Notch receptor in a cis and trans fashion manner. Following transinteraction, ligand cells produce mechanical force that depends of a clathrin-mediated endocytosis, requiring ligand ubiquitination, EPN1 interaction, and actin polymerisation; these events promote Notch receptor extracellular domain (NECD) transendocytosis and triggers Notch signaling through induction of cleavage, hyperphosphorylation, and nuclear accumulation of the intracellular domain of Notch receptors (NICD). Is required for embryonic development and maintenance of adult stem cells in many different tissues and immune systeme; the DLL1-induced Notch signaling is mediated through an intercellular communication that regulates cell lineage, cell specification, cell patterning and morphogenesis through effects on differentiation and proliferation. Plays a role in brain development at different level, namely by regulating neuronal differentiation of neural precursor cells via cell-cell interaction, most likely through the lateral inhibitory system in an endogenous level dependent-manner. During neocortex development, Dll1-Notch signaling transmission is mediated by dynamic interactions between intermediate neurogenic progenitors and radial glia; the cell-cell interactions are mediated via dynamic and transient elongation processes, likely to reactivate/maintain Notch activity in neighboring progenitors, and coordinate progenitor cell division and differentiation across radial and zonal boundaries. During cerebellar development, regulates Bergmann glial monolayer formation and its morphological maturation through a Notch signaling pathway. At the retina and spinal cord level, regulates neurogenesis by preventing the premature differentiation of neural progenitors and also by maintaining progenitors in spinal cord through Notch signaling pathway. Also controls neurogenesis of the neural tube in a progenitor domain-specific fashion along the dorsoventral axis. Maintains quiescence of neural stem cells and plays a role as a fate determinant that segregates asymmetrically to one daughter cell during neural stem cells mitosis, resulting in neuronal differentiation in Dll1-inheriting cell. Plays a role in immune systeme development, namely the development of all T-cells and marginal zone (MZ) B cells. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor. Upon MMP14 cleavage, negatively regulates Notch signaling in haematopoietic progenitor cells to specifically maintain normal B-cell development in bone marrow. Also plays a role during muscle development. During early development, inhibits myoblasts differentiation from the medial dermomyotomal lip and later regulates progenitor cell differentiation. Directly modulates cell adhesion and basal lamina formation in satellite cells through Notch signaling. Maintains myogenic progenitors pool by suppressing differentiation through down-regulation of MYOD1 and is required for satellite cell homing and PAX7 expression. During craniofacial and trunk myogenesis suppresses differentiation of cranial mesoderm-derived and somite-derived muscle via MYOD1 regulation but in cranial mesoderm-derived progenitors, is neither required for satellite cell homing nor for PAX7 expression. Also plays a role during pancreatic cell development. During type B pancreatic cell development, may be involved in the initiation of proximodistal patterning in the early pancreatic epithelium. Stimulates multipotent pancreatic progenitor cells proliferation and pancreatic growth by maintaining HES1 expression and PTF1A protein levels. During fetal stages of development, is required to maintain arterial identity and the responsiveness of arterial endothelial cells for VEGFA through regulation of KDR activation and NRP1 expression. Controls sprouting angiogenesis and subsequent vertical branch formation througth regulation on tip cell differentiation. Negatively regulates goblet cell differentiation in intestine and controls secretory fat commitment through lateral inhibition in small intestine. Plays a role during inner ear development; negatively regulates auditory hair cell differentiation. Plays a role during nephron development through Notch signaling pathway. Regulates growth, blood pressure and energy homeostasis.
  • 基因功能参考文献：
    1. this study identified Notch ligand Dll1 as amarker that is enriched in mammary gland stem celsl(MaSCs) and demonstrated that Dll1+ MaSCs can generate both basal and luminal cells. PMID: 29773667
    2. Study show how delta-like protein 1 (DLK1) has a certain role in the interactions between the adipose tissue and the adenohypophyseal cells. DLK1 can directly or indirectly mediate in pituitary leptin production in growth hormone and prolactin cells. PMID: 28718206
    3. Activation of Notch signaling with the endothelial cell (EC)-targeted soluble Dll1 Notch ligand mD1R promoted bone marrow recovery after irradiation. PMID: 27188577
    4. Macrophage maturation is controlled by Notch ligand Dll1 expressed in vascular endothelial cells of arteries and requires macrophage canonical Notch signaling via Rbpj, which simultaneously suppresses an inflammatory macrophage fate. Conversely, conditional mutant mice lacking Dll1 or Rbpj show proliferation and transient accumulation of inflammatory macrophages, which antagonizes arteriogenesis and tissue repair. PMID: 29038527
    5. pre-coated Notch1 protein promotes Notch1-knocked down B cells to produce antibody in LPS-stimulated B cells suggesting that Notch1 in other cells may promote antibody production by binding its ligands Dll1 and Jag1 in B cells. PMID: 28863329
    6. Hedgehog/Patched-associated rhabdomyosarcoma formation from delta1-expressing mesodermal cells PMID: 26387541
    7. the effects of two Notch ligands, i.e., Jagged1 and DLL1, on murine and human hematopoiesis in vitro. Our observations indicate that the stromal expression of Notch ligands increases the production of both the total and phenotypically early murine and human hematopoietic cells in the co-culture. PMID: 28537242
    8. Fringe modifications at EGF8 and EGF12 enhanced Notch1 binding to and activation from Delta-like 1, while modifications at EGF6 and EGF36 (added by Manic and Lunatic but not Radical) inhibited Notch1 activation from Jagged1. PMID: 28089369
    9. the structural integrity of each individual EGF repeat in the extracellular domain of DLL1 is necessary for full DLL1 activity PMID: 26801181
    10. Role of Dll1 in embryogenesis and notch signaling. PMID: 26114479
    11. the appropriate timing of Dll1 expression is critical for the oscillatory networks and suggest the functional significance of oscillatory cell-cell interactions in tissue morphogenesis PMID: 26728556
    12. Spatiotemporal expression oscillations of Notch1 and Dll1 are coordinated across the mouse pre-somitic mesoderm. PMID: 25468943
    13. Dll1 functions in satellite cell homing and in Pax7 expression diverge in cranial- vs. somite-derived muscle. PMID: 25220152
    14. mDLL1 O-fucosylation by POFUT1 is dispensable for ligand function PMID: 24533113
    15. suppression of IL-6, GM-CSF, and MMP-3 production by DLL-1 blockade might be responsible for the amelioration of arthritis in a mouse model of rheumatoid arthritis. PMID: 24943093
    16. Normal development in mice over-expressing the intracellular domain of DLL1 argues against reverse signaling by DLL1 in vivo. PMID: 24167636
    17. A DLL1 variant in which three phosphorylated serine/threonine residues are mutated to alanine and valine, was more stable than wild-type DLL1 but had reduced relative levels on the cell surface and was more effectively cleaved in the extracellular domain. PMID: 24449764
    18. cells expressing transfected protein could support T-cell lineage differentiation PMID: 23988616
    19. The transcription and expression patterns of Notch pathway components (Notch 1-3, Delta1 and 4, Jagged1) and effectors (Hes1, Hes2, Hes5 and Nrarp) were evaluated in the mouse testis PMID: 24015274
    20. The Notch ligand Delta-like 1 (Dll1) is required to maintain quiescent neural stem cells in the adult mouse subventricular zone. PMID: 23695674
    21. Dll1 is required for the formation of the Bergmann layer and its morphological maturation. PMID: 23688253
    22. Our finding that DLL1 is a product of immature and mature uNK cells suggests it would be profitable to explore the roles of other ILC subsets in the promotion of angiogenesis and in particular in the induction of endothelial tip cell differentiation. PMID: 23284862
    23. Delta-like 1 is involved in the differential regulation of osteoclastogenesis. PMID: 22390640
    24. Different levels of Dll1 expression determine the fate of neural precursor cells through cell-cell interactions, most likely through the Notch-Delta lateral inhibitory signaling pathway, thus contributing to the selection of differentiating cells. PMID: 18997111
    25. Dll1 and Dll4 function sequentially in the retina and pV2 domain of the spinal cord to regulate neurogenesis and create cell diversity. PMID: 19389377
    26. data suggest that the development of human B lymphocytes and HSC maintenance in osteosclerotic bone may be suppressed by introducing DLL1 PMID: 22771497
    27. We examined the role of Notch ligand Delta-like 1 (Delta1) in the development of Theiler's murine encephalomyelitis virus (TMEV)-induced demyelinating disease ( PMID: 22944320
    28. Notch1 activity in gastric cancer is controlled by the epigenetic silencing of the ligand DLL1, and that Notch1 inhibition is associated with the diffuse type of gastric cancer. PMID: 22249198
    29. MiR-34a targeting of Notch ligand delta-like 1 impairs CD15+/CD133+ tumor-propagating cells and supports neural differentiation in medulloblastoma PMID: 21931765
    30. results suggest that Notch signaling through macrophage-dependent Dll1 is critical in providing an anti-viral response during influenza infection by linking innate and acquired immunity PMID: 22072963
    31. the presence of Gal on O-fucose glycans differentially affects DLL1-induced NOTCH signaling modulated by LFNG versus MFNG PMID: 22081605
    32. Dll1 acts as an extrinsic cue involved in tip cell selection, which directs vessel sprouting and branch formation. PMID: 22282195
    33. Data find that only secretory cells pass through a phase of strong expression of the Notch ligand Delta1. Onset of this Dll1 expression coincides with a block to further cell division and is followed by expression of Neurog3 or Gfi1. PMID: 21915337
    34. Ptf1a-induced Dll1 expression stimulates multipotent pancreatic progenitor cells proliferation and pancreatic growth by maintaining Hes1 expression and Ptf1a protein levels. PMID: 22096075
    35. Anti-delta1 monoclonal antibody-treated heart transplants are a potential target in this study to improve graft-prolonging effects of Notch ligand delta1. PMID: 21949024
    36. Dll1 can promote the differentiation of DCs from mouse bone marrow cells and enhance their antigen presentation capacity. PMID: 20561433
    37. overexpression of Dll1 could activate Notch signaling in tumor cells, and promote tumor cell proliferation in vitro PMID: 21752535
    38. Elav-like (Elavl1)/HuR becomes enriched in the cytoplasm of neuroepithelial cells undergoing mitosis and that this ribonucleoprotein binds the 3' untranslated region (UTR) of Dll1 mRNA and stabilizes it. PMID: 21346194
    39. These data suggest that MT1-MMP directly cleaves Dll1 on bone marrow stromal cells to negatively regulate Notch signalling to specifically maintain normal B-cell development in bone marrow. PMID: 21572390
    40. Data suggest that selective inhibition of Notch signalling mediated by Notch3 or Delta-like1 may offer a new strategy for the treatment of RA. PMID: 21235539
    41. Notch signaling in stem cells and progenitors is activated by Dll1 and Dll4 ligands and is required for maintenance of intestinal progenitor and stem cells PMID: 21238454
    42. Results reveal that Dll1 and Jag1 are functionally equivalent in controlling the rate of neurogenesis within their expression domains. PMID: 21124801
    43. Proteolytic cleavage of Dll1 in Notch signal sending cells is dispensable for murine T-cell development, differentially affects expression of Notch target genes, and might be a mechanism that regulates myelopoiesis. PMID: 20231851
    44. Identification of miRNAs could possibly target the Dll1 3'UTR in these regions. PMID: 20558326
    45. Notch receptor(s) and Dll1 directly promote the adhesion of mast cells to stromal cells by acting as adhesion molecules. PMID: 20810995
    46. Findings show that Dll4 expressed on OP9 cells is functionally distinct from Dll1, illustrating the unique properties of Dll4 in supporting the combined T lineage and specific myeloid-lineage outcomes that underpin its function within the thymus. PMID: 20548034
    47. Notch ligands, including Delta-like1 and 3 and Jagged1 and Jagged2, show distinct expression patterns in the developing and adult brain overlapping that of Notch1 PMID: 12175503
    48. Delta-1 is essential for proper migration and differentiation of neural crest cells. Interestingly, absence of Delta-1 leads to diminution of both neurons and glia in peripheral ganglia PMID: 12217323
    49. the loss-of-function of the delta 1 (Dll1) gene causes a randomisation of the direction of heart looping and embryonic turning. The most probable cause for this defect in Dll1 mutant embryos is a failure in the development of proper midline structures PMID: 12441287
    50. identification as substrated for presenilin-dependent gamma-secretase cleavage PMID: 12551931

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  • 亚细胞定位：
    Apical cell membrane; Single-pass type I membrane protein. Cell junction, adherens junction. Membrane raft.; [Dll1-derived cell-associated form]: Cell membrane.; [Dll1-intracellular form]: Nucleus.
  • 组织特异性：
    In the embryo, expressed in the paraxial mesoderm and nervous system. Expressed at high levels in adult heart and at lower levels, in adult lung. Highly expressed in satellite cells from masseter and tongue than in satellite cells from leg and extraocular
  • 数据库链接：

    KEGG: mmu:13388

    STRING: 10090.ENSMUSP00000014917

    UniGene: Mm.4875