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  4. Recombinant Mouse DNA replication licensing factor MCM7 (Mcm7)

Recombinant Mouse DNA replication licensing factor MCM7 (Mcm7)

  • 货号：
    CSB-YP737046MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP737046MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP737046MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP737046MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP737046MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Mcm7; Cdc47; Mcmd7DNA replication licensing factor MCM7; EC 3.6.4.12; CDC47 homolog
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-719
  • 氨基酸序列
    ALKDYAIEK EKVKKFLQEF YYENELGKKQ FKYGTQLVHL AHREQVALYV DLDDIAEDDP ELVDSICENA KRYSRLFGDV VQELLPEYKE KEVVNKDVLD VYIEHRLMME QRSRDPGAVR NPQNQYPSEL MRRFELYFRG PSSSKPRVIR EVRADSVGKL LTVRGIVTRV SEVKPRMVVA TYTCDQCGAE TYQPIQSPTF MPLIMCPSQE CQTNRSGGRL YLQTRGSKFV KFQEMKIQEH SDQVPVGNIP RSITVVLEGE NTRIAQPGDH VSVTGIFLPV LRTGFQQMAQ GLLSETYLEA HWIVKMTKSD DDVSGAGELS SEELKQIAEE DFYEKLAASI APEIYGHEDV KKALLLLLVG GVDQSPQGMK IRGNIHICLM GDPGVAKSQL LSYIDRLAPR SQYTTGRGSS GVGLTAAVLR DSVSGELTLE GGALVLADQG VCCIDEFDKM AEADRTAIHE VMEQQTISIA KAGILTTLNA RCSILAAANP AYGRYNPRRS LEQNVQLPAA LLSRFDLLWL IQDRPDRDND LRLAQHITYV HQHSRQPPAQ FEPLDMKLMR RYIAMCHERQ PTVPESLADY ITAAYVEMRR EARASKDATY TSARTLLAIL RLSTALARLR MVDIVEKEDV NEAIRLMEMS KDSLLGEKGQ TARTQRPADV IFATIRELVS RGRSVHFSEA EQRCISRGFT PAQFQAALDE YEELNVWQVN TSRTRITFV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.
  • 基因功能参考文献：
    1. Unique pattern of ORC2 and MCM7 localization during DNA replication licensing in the mouse zygote. PMID: 22674395
    2. role of subunits in DNA helicases activity PMID: 12207017
    3. cyclin D1/CDK4 complexes play a direct role in altering an inhibitory RB.MCM7 complex possibly allowing for setting of the origin in preparation for DNA replication PMID: 12519773
    4. MCM7 may be a highly informative biomarker for cervical cancer. PMID: 14678972
    5. Accumulation and dynamics of Mcm7 are described during oogenesis and the first embryonic cell cycle. PMID: 17554680
    6. cellular DNA replication factor MCM7 is involved in prostate cancer (CaP) and MCM7 gene expression was reduced by green tea catechins PMID: 18521193

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  • 亚细胞定位：
    Nucleus. Chromosome.
  • 蛋白家族：
    MCM family
  • 数据库链接：

    KEGG: mmu:17220

    STRING: 10090.ENSMUSP00000000505

    UniGene: Mm.378965