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  4. Recombinant Mouse DNA repair protein XRCC1 (Xrcc1)

Recombinant Mouse DNA repair protein XRCC1 (Xrcc1)

  • 货号：
    CSB-YP726654MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP726654MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP726654MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP726654MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP726654MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Xrcc1; Xrcc-1; DNA repair protein XRCC1; X-ray repair cross-complementing protein 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-631
  • 氨基酸序列
    MPEISLRHVV SCSSQDSTHC AENLLKADTY RKWRAAKAGE KTISVVLQLE KEEQIHSVDI GNDGSAFVEV LVGSSAGGAT AGEQDYEVLL VTSSFMSPSE SRSGSNPNRV RIFGPDKLVR AAAEKRWDRV KIVCSQPYSK DSPYGLSFVK FHSPPDKDEA EATSQKVTVT KLGQFRVKEE DDSANSLKPG ALFFSRINKT SSASTSDPAG PSYAAATLQA SSAASSASPV PKVVGSSSKP QEPPKGKRKL DLSLEDRKPP SKPSAGPSTL KRPKLSVPSR TPAAAPASTP AQRAVPGKPR GEGTEPRGAR TGPQELGKIL QGVVVVLSGF QNPFRSELRD KALELGAKYR PDWTPDSTHL ICAFANTPKY SQVLGLGGRI VRKEWVLDCH HMRRRLPSRR YLMAGLGSSS EDEGDSHSES GEDEAPKLPQ KRPQPKAKTQ AAGPSSPPRP PTPKETKAPS PGPQDNSDTE GEESEGRDNG AEDSGDTEDE LRRVAKQREQ RQPPAPEENG EDPYAGSTDE NTDSETPSEA DLPIPELPDF FEGKHFFLYG EFPGDERRRL IRYVTAFNGE LEDYMNERVQ FVITAQEWDP NFEEALMENP SLAFVRPRWI YSCNEKQKLL PHQLYGVVPQ A
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in DNA single-strand break repair by mediating the assembly of DNA break repair protein complexes. Probably during DNA repair, negatively regulates ADP-ribose levels by modulating ADP-ribosyltransferase PARP1 activity.
  • 基因功能参考文献：
    1. Unrepaired single strand DNA breaks (SSBs) activate DNA damage response and increase the expression of inflammatory cytokines through NF-kappaB signalling. Pressure overload-induced heart failure is more severe in the mice lacking XRCC1, an essential protein for SSB repair. PMID: 28436431
    2. Interaction with phosphorylated XRCC1 is a requirement for significant APTX recruitment to cellular DNA damage and enzymatic activity in cell extracts. PMID: 29477978
    3. this review focuses on the role of the oxidized form of XRCC1 in protection against extreme oxidative stress PMID: 28179111
    4. Repair independent of the well documented XRCC1-PNKP interaction was studied. XRCC1 can mediate repair of strand breaks without PNKP binding. PMID: 29100039
    5. data establish the importance of XRCC1 protein complexes for normal neurological function and identify PARP1 as a therapeutic target in DNA strand break repair-defective disease PMID: 28002403
    6. We have characterized the nuclear localization signal (NLS) of XRCC1 structurally using X-ray crystallography and functionally using fluorescence imaging. PMID: 26304019
    7. Its allelic loss results in increased brain damage and reduced recovery from ischemic stroke. PMID: 25971543
    8. Data indicate that maternal folate depletion during pregnancy and high-fat feeding from weaning altered gene expression of Ogg1, Neil1, Mutyh and Xrcc1 in the brain of adult offspring. PMID: 23603834
    9. In cells with DNA base damage, PAR serves to recruit XRCC1 that in turn binds and recruits pol beta, the primary DNA polymerase of the base excision repair pathway. PMID: 23871146
    10. Lig4 and XRCC1 double-deficient cells switch as efficiently as Lig4-deficient cells, clearly indicating that XRCC1 is dispensable for A-EJ in CH12F3 cells during class switch recombination PMID: 22392994
    11. findings firmly demonstrate that XRCC1 is not a requisite factor for A-EJ of chromosomal DSBs and raise the possibility that DNA ligase 1 (Lig1) may contribute more to A-EJ than previously considered PMID: 22308491
    12. Data support a role for XRCC1 in microhomology-mediated joining, and imply that AID-induced single-strand breaks in Igh variable and switch regions become substrates simultaneously for BER and mutagenesis pathways. PMID: 21967769
    13. Results indicates that XRCC1 haploinsufficiency has little effect on chronological longevity and many key biological markers of aging, but may adversely affect normal animal development or increase disease susceptibility to a relevant genotoxic exposure. PMID: 21737425
    14. data reveal that the critical biological role of Lig3 is to maintain mtDNA integrity and not Xrcc1-dependent DNA repair PMID: 21390131
    15. results establish a role for Lig3 in mitochondria, but distinguish it from its interacting protein Xrcc1 PMID: 21390132
    16. poly(ADP-ribose) polymerase-1 and XRCC1/DNA ligase III utilize an alternative route for DNA double-strand breaks rejoining PMID: 15498778
    17. These results identify a novel role for FoxM1 in the transcriptional response during DNA damage/checkpoint signaling and show a novel mechanism by which Chk2 protein regulates expression of DNA repair enzymes. PMID: 17101782
    18. the XRCC1 Arg399Gln and RAD51 5'UTR G135C polymorphisms have roles in breast cancer aggressiveness PMID: 17616806
    19. E2F1 regulates the base excision repair gene XRCC1 and promotes DNA repair PMID: 18348985

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  • 亚细胞定位：
    Nucleus. Nucleus, nucleolus.
  • 数据库链接：

    KEGG: mmu:22594

    STRING: 10090.ENSMUSP00000070995

    UniGene: Mm.4347