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  4. Recombinant Mouse DNA polymerase iota (Poli)

Recombinant Mouse DNA polymerase iota (Poli)

  • 货号：
    CSB-YP750922MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP750922MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP750922MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP750922MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP750922MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Poli; Rad30b; DNA polymerase iota; EC 2.7.7.7; Rad30 homolog B
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-717
  • 氨基酸序列
    MEPLHAGAAG SSRAVCSQGP PTQISSSRVI VHVDLDCFYA QVEMISNPEL KDRPLGVQQK YLVVTCNYEA RKLGVRKLMN VRDAKEKCPQ LVLVNGEDLS RYREMSYKVT ELLEEFSPAV ERLGFDENFV DLTEMVEKRL QQLPSEEVPS VTVFGHVYNN QSVNLHNIMH RRLVVGSQIA AEMREAMYNQ LGLTGCAGVA PNKLLAKLVS GVFKPNQQTV LLPESCQHLI HSLNHIKEIP GIGYKTAKRL EVLGINSVHD LQTFPIKTLE KELGIAIAQR IQQLSFGEDK SPVTPSGPPQ SFSEEDTFKK CSSEVEAKAK IEELLSSLLT RVCQDGRKPH TVRLVIRRYS DKHCNRESRQ CPIPSHVIQK LGTGNHDSMP PLIDILMKLF RNMVNVKMPF HLTLMSVCFC NLKALSSAKK GPMDCYLTSL STPAYTDKRA FKVKDTHTED SHKEKEANWD CLPSRRIEST GTGESPLDAT CFPKEKDTSD LPLQALPEGV DQEVFKQLPA DIQEEILYGK SRENLKGKGS LSCPLHASRG VLSFFSTKQM QASRLSPRDT ALPSKRVSAA SPCEPGTSGL SPGSTSHPSC GKDCSYYIDS QLKDEQTSQG PTESQGCQFS STNPAVSGFH SFPNLQTEQL FSTHRTVDSH KQTATASHQG LESHQGLESR ELDSAEEKLP FPPDIDPQVF YELPEEVQKE LMAEWERAGA ARPSAHR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.
  • 基因功能参考文献：
    1. Pol iota occasionally accesses the replication fork to generate a first mutation, and Pol zeta extends the mismatch with a second mutation. PMID: 27455952
    2. Mouse DNA polymerase iota lacking exon 2 (42 amino acids) is catalytically inactive in vitro. PMID: 28077247
    3. Exon 2 deletion abrogates both the DNA polymerase and dRP lyase activities of Pol iota in the presence of either Mg(2+) or Mn(2+) ions. Thus, 129-derived strains of mice express catalytically inactive alternatively spliced Pol iota variant. PMID: 28077248
    4. PolH contributes to accurate translesion synthesis (TLS) past both T- & C-containing dimers. PolI is involved in error-prone TLS past cytosine-containing dimers when Poleta is inactivated. PMID: 25733082
    5. Poliota causes the Par2 effect and inhibits tumorigenesis and mutagenesis PMID: 23955086
    6. in mammalian cells, both polymerases kappa and iota are necessary for the error-free bypass of N(2)-CEdG and N(2)-CMdG. PMID: 21454642
    7. Upon DNA damage, the UBDs (UBM domains) of polymerase iota (Pol iota) interact with ubiquitinated proliferating cell nuclear antigen to regulate the interchange between processive DNA polymerases and translesional synthesis PMID: 20929865
    8. In the presence of Mg2+, the enzyme was active only in testicles and brain, whereas in the presence of Mn2+ the activity was found in all organs. PMID: 20673215
    9. Data suggest that either DNA polymerase iota does not participate in hypermutation, or its role is nonessential and can be readily assumed by another low-fidelity polymerase. PMID: 12925679
    10. Nucleotide polymorphisms in DNA polymerase iota is associated with lung tumorigenesis in mouse and humans PMID: 15026325
    11. Pol kappa and Pol iota double-deficient mice had the normal somatic hypermutation frequency PMID: 15860226
    12. Pol iota gene may participate in error-free repair of damaged DNA and prevention of lung tumor development PMID: 15904465
    13. identification of two previously unknown ubiquitin-binding domains in the Y-family translesion synthesis polymerases that enable them to interact with monoubiquitinated targets and undergo monoubiquitination in vivo PMID: 16357261
    14. suggest the involvement of the Pol eta and Pol iota proteins in UV-induced skin carcinogenesis PMID: 17015482
    15. Although pol iota deficiency alone had no effect, UV-induced skin tumors in pol eta-deficient mice developed 4 weeks earlier in mice concomitantly deficient in pol iota PMID: 17114294

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    DNA polymerase type-Y family
  • 组织特异性：
    Detected in testis, and at very low levels in spleen, lung and brain. Detected in round spermatids, but not in prophase spermatocytes.
  • 数据库链接：

    KEGG: mmu:26447

    STRING: 10090.ENSMUSP00000112563

    UniGene: Mm.244352