Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Mouse DNA excision repair protein ERCC-8 (Ercc8)

Recombinant Mouse DNA excision repair protein ERCC-8 (Ercc8)

  • 货号：
    CSB-YP804034MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP804034MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP804034MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP804034MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP804034MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Ercc8; Ckn1; CsaDNA excision repair protein ERCC-8; Cockayne syndrome WD repeat protein CSA homolog
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-397
  • 氨基酸序列
    MLSFLSARQS GLEDPLRLRR AQSTRRVLGL ELNKDRDVER IHGSGVNTLD IEPVEGRYML SGGSDGVVVL YDLENASRQP HYTCKAVCSV GRSHPDVHKY SVETVQWYPH DTGMFTSSSF DKTLKVWDTN TLQAADVFNF EETVYSHHMS PAATKHCLVA VGTRGPKVQL CDLKSGSCSH ILQGHRQEIL AVSWSPRHDY ILATASADSR VKLWDVRRAS GCLLTLDQHN GKKSQAAESA NTAHNGKVNG LCFTSDGLHL LTIGTDNRMR LWNSSSGDNT LVNYGKVCND SRKGLQFAVS CGCSSEFVFV PHGSTIAVYA VHSGERLAML KGHYKSVDCC VFQPNFQELY SGSRDCNILA WVPPSYEPVP DDDDEAPAKS QLNPAFADAW SSSDEDG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Substrate-recognition component of the CSA complex, a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, involved in transcription-coupled nucleotide excision repair. The CSA complex (DCX(ERCC8) complex) promotes the ubiquitination and subsequent proteasomal degradation of ERCC6 in a UV-dependent manner; ERCC6 degradation is essential for the recovery of RNA synthesis after transcription-coupled repair. It is required for the recruitment of XAB2, HMGN1 and TCEA1/TFIIS to a transcription-coupled repair complex which removes RNA polymerase II-blocking lesions from the transcribed strand of active genes. Plays a role in DNA single-strand and double-strand breaks (DSSBs) repair; involved in repair of DSSBs by non-homologous end joining (NHEJ).
  • 基因功能参考文献：
    1. This study demonistrated that Cockayne syndrome group A deficiencies predispose to hearing loss and cochlear hair cell degeneration in mice. PMID: 25762674
    2. MtDNA mutations are highly increased in cells from subcutaneous fat of aged Csa(-/-) mice PMID: 20100872
    3. difference in oxidative DNA damage sensitivity between CSA- and CSB-deficient cell lines and mice PMID: 15340056
  • 亚细胞定位：
    Nucleus. Nucleus matrix.
  • 数据库链接：

    KEGG: mmu:71991

    STRING: 10090.ENSMUSP00000059211

    UniGene: Mm.212208