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  4. Recombinant Mouse Cyclin-dependent kinase inhibitor 1 (Cdkn1a)

Recombinant Mouse Cyclin-dependent kinase inhibitor 1 (Cdkn1a)

  • 货号：
    CSB-YP005086MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP005086MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP005086MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP005086MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Cdkn1a; Cip1; Waf1Cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; Melanoma differentiation-associated protein; p21
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-159
  • 氨基酸序列
    SNPGDVRPV PHRSKVCRCL FGPVDSEQLR RDCDALMAGC LQEARERWNF DFVTETPLEG NFVWERVRSL GLPKVYLSPG SRSRDDLGGD KRPSTSSALL QGPAPEDHVA LSLSCTLVSE RPEDSPGGPG TSQGRKRRQT SLTDFYHSKR RLVFCKRKP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding. Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest.
  • 基因功能参考文献：
    1. Gene expressions in lung tissues from systemic bleomycin-treated mice were examined, revealing significant increased expression of Cdkn1alpha (a gene coding for p21), particularly in distal regions of the lung. PMID: 29386571
    2. Study reveal that p16Ink4a and p21Waf1/Cip1 upregulate CX3CR1 expression by preventing CDK-mediated phosphorylation and inactivation of SMAD3 in monocytic myeloid-derived suppressor cells promoting tumor growth through chemotaxis. PMID: 29234059
    3. while muCT analysis indicates that p21(-/-) mice have enhanced bone healing capabilities, differences observed may not be due to the function of osteoblasts or osteoclasts PMID: 29121899
    4. the deletion of Cdkn1a in vivo results in an elevated level of cell proliferation but not cell death. PMID: 29777010
    5. p21 plays a relevant role in fasting adaptation through the positive regulation of PPARalpha. PMID: 27721423
    6. The results suggested that TAZ may suppress apoptosis and premature senescence in spermatogenic cells by inhibiting the p53-p21 signaling pathway, thus playing important roles in the maintenance and control of reproductive function. PMID: 28613007
    7. Results identify endoplasmic reticulum stress as an age-dependent modifier of islet survival and function by mechanisms implicating enhancement of CHOP activity and inhibition of the protective activity of p21. PMID: 27931122
    8. Specifically, the proteins p18INK4C, p21CIP1 and p27KIP1 seem to play an outstanding role in the maintenance of the differentiated state of adipocytes. PMID: 28287250
    9. Collectively, our findings demonstrate that PCAT-1 is a new candidate for use in OS diagnosis, prognosis and therapy. PMID: 29291409
    10. results indicated that p21-deficient DMM mice were susceptible to alterations in Osteoarthritis (OA) phenotype, including enhanced osteoclast expression, macrophage infiltration, and MMP expression through IL-1beta-induced NF-kappaB signaling, suggesting that p21 regulation may constitute a possible therapeutic strategy for OA treatment. PMID: 28128866
    11. our genetic and biochemical data show an important function of p21 in the regulation of growth-related processes in the heart. PMID: 27486069
    12. Data suggest BAF180 protein as a critical regulator of cellular senescence and HSC homeostasis, which is at least partially regulated through BAF180-mediated suppression of cell cycle regulator p21 expression. PMID: 26992241
    13. Histone methyltransferase Suv39h1 attenuates high glucose-induced fibronectin and p21(WAF1) in mesangial cells PMID: 27373678
    14. It has been observed that even in tissues with no detectable Linc-p21 transcript, deletion of the locus significantly affects local gene expression, including of the cell cycle regulator Cdkn1a. PMID: 27524623
    15. The findings suggest that p21 facilitates the development of cardiac hypertrophy, and regulating the expression of p21 may be an approach to attenuate hypertrophic growth of cardiomyocytes. PMID: 28746924
    16. The study demonstrates an essential role of Setd2 in myoblast proliferation and differentiation, and uncovers Setd2-mediated molecular mechanism through regulating MyoG and p21. PMID: 28130125
    17. Schistosoma japonicum egg antigen p40 through action on the STAT3/p53/p21 pathway triggered cellular senescence, while knockdown of p53 or STAT3 partly restored cell senescence. PMID: 27468691
    18. Data show that Emu-Myc mice lacking both p21 and PUMA developed lymphoma at a rate considerably longer latency than Emu-Myc;p53(+/-)mice. PMID: 26640149
    19. Study reports that p27 normally exerts a negative feedback on p21 expression: p27 directly represses the expression of the transcription factor Pitx2 which in turn maintains decreased p21 levels. Consequently, in cells lacking p27, de-repression of Pitx2 causes the up-regulation of p21 showing a new mechanism by which p27 regulates cell cycle progression by transcriptionally regulating the expression of Pitx2 and p21. PMID: 27270438
    20. p21-associated inhibition of early-stage malignant progression and the intense expression in papilloma outgrowths, identifies a novel, significant antagonism between p21 and ras(Ha)/ROCK2/NF-kappaB signalling in skin carcinogenesis.these data show that ROCK2 activation induces malignancy in ras(Ha)-initiated/promoted papillomas in the context of p53 loss and novel NF-kappaB expression PMID: 27991921
    21. both in vitro and in vivo studies proved that CypD inhibitor-based treatment was able to efficiently impair this interaction, leading to a tumor formation reduction. All together, these findings indicate that the countering effect of CypD on the p53-p21 pathway participates in oncogene-dependent transformation. PMID: 26973251
    22. FLT3-ITD is capable of inhibiting FLT3-ITD+ cell proliferation through the p21/Pbx1 axis PMID: 27387666
    23. These data reveal a novel role for p21/waf1 in the resolution of inflammation via its ability to control neutrophil apoptosis. PMID: 26517580
    24. the silencing of Cyclin-dependent kinase 5 preventing memory dysfunction PMID: 27273428
    25. Results show that p21 expression is regulated by OLA1 to promote cell proliferation and organogenesis. PMID: 27481995
    26. AS160 regulates glucose-independent eukaryotic cell proliferation through p21-dependent control of the cell cycle. PMID: 27152871
    27. telomerase activity and cardiomyocyte telomere length decrease sharply in wild-type mouse hearts after birth, resulting in cardiomyocytes with dysfunctional telomeres and anaphase bridges and positive for the cell-cycle arrest protein p21. PMID: 27241915
    28. Consistent with the latter, Arid1a reexpression in tumor cells led to increased p21 (Cdkn1a) expression and dramatic accumulation of cells in G2 phase of the cell cycle. These results also indicate a potential opportunity for therapeutic intervention in ARID1A-deficient human breast cancer subtypes that retain one intact copy of the gene and also maintain wild-type TRP53 activity PMID: 27280691
    29. We discovered that FXR1P-deficient aNSCs have altered expression of a select number of cell-cycle genes, and we identified the mRNA of cyclin-dependent kinase inhibitor 1A (Cdkn1a, p21) as a direct target of FXR1P. PMID: 28204491
    30. These results demonstrate that CQ and AQ increase the expression level of p21 and inhibit T cell proliferation and the development of IFN-gamma-producing Th1 cells, thereby revealing beneficial roles in treating a wide range of chronic inflammatory diseases mediated by inflammatory T cells. PMID: 27109480
    31. increases the number of ALP-positive colonies after iPSC induction and decreases expression levels of Eomes and p21 mRNAs. Based on these observations, we propose that the CCR4-NOT deadenylase activity contributes to iPSC induction. PMID: 27037025
    32. this study shows that p21 is upregulated in T effector cells but not natural regulatory T cells after treatment with azacitidine, which protects from graft-versus-host Disease PMID: 28330901
    33. lincRNA-p21 overexpression dramatically inhibited acetylation of H3 and H4 at the Thy-1 promoter and Thy-1 expression levels in HLF1 cells. Finally, lincRNA-p21 interference rescued LPS-induced increase of lung and BAL collagen contents. LincRNA-p21 could lead to pulmonary fibrosis in ARDS by inhibition of the expression of Thy-1. PMID: 27392907
    34. p21 upregulation in hair follicle stem cells is associated with telogen retention in aged mice PMID: 26439682
    35. MiR-17-92 functions as a hepatocyte proliferation stimulator and acts in an estrogen-dependent manner. The loss of this miRNA results in increases in p21 and Pten expression and therefore impairs liver regeneration in female mice. PMID: 26781774
    36. we observed that miR-378 modulates the oscillation amplitudes of Cdkn1a in the control of cell cycle and Por in the regulation of oxidation reduction by forming partnership with different circadian transcription factors PMID: 26898952
    37. The findings define PRMT7 as a regulator of the DNMT3b/p21 axis required to maintain muscle stem cell regenerative capacity. PMID: 26854227
    38. Dynamics of CDKN1A in single cells has been defined defined by an endogenous fluorescent tagging. PMID: 26876176
    39. Ablation of p21Cip1 in the Cdk4 R24C background accelerates mesenchymal tumor development in mice. PMID: 26292757
    40. The lack of functional Waf1 is indispensable for maintaining self-renewal and pluripotency of embryonic stem cells. PMID: 26636245
    41. p27(Kip1) and p21(Cip1) are insufficient for the proliferative inhibition of smooth muscle cells cultured in type 1 collagen. PMID: 26522285
    42. TFAP2C in trophoblasts controls proliferation by repressing Cdkn1a and activating the MAPK pathway, further supporting differentiation of glycogen cells by activating the AKT pathway PMID: 26811378
    43. we present evidence that VPA administration to mice increases hippocampal p21 expression, accompanied by DNA demethylation at the distal CpG island. PMID: 26339990
    44. LKB1 mediates CDKN1A degradation in response to ultraviolet DNA damage. PMID: 25329316
    45. At high glucose concentrations in vitro, AdipoR1 regulated the survival of neural stem cells through the p53/p21 pathway and the proliferation- and differentiation-related factors of neural stem cells via TLX. PMID: 26247729
    46. Phosphorylation of p53 by LRRK2 induces p21(WAF1/CIP1) expression and apoptosis. PMID: 26384650
    47. AMP-18 appears to act through PI3K/AKT pathways to increase p21 phosphorylation, thereby reducing its nuclear accumulation to overcome the antiproliferative effects of TNF-alpha. PMID: 25919700
    48. Intense P21 expression observed in basal and supra-basal layers facing future dental papilla cells, weakly expressed in other basal cells. P21 is exclusively found in the IEE, supra-IEE and several dental papilla cells in mice. PMID: 26042621
    49. Deficiency of CDKN1A or both CDKN1A and CDKN1B affects the pubertal development of mouse Leydig cells. PMID: 25609837
    50. we conclude that p21 plays an important role in the in vivo healing process in muscular injury. PMID: 25942471

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    CDI family
  • 数据库链接：

    KEGG: mmu:12575

    STRING: 10090.ENSMUSP00000023829

    UniGene: Mm.195663