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  4. Recombinant Mouse Cyclin-T1 (Ccnt1)

Recombinant Mouse Cyclin-T1 (Ccnt1)

  • 货号：
    CSB-YP874100MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP874100MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP874100MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP874100MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP874100MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Ccnt1
  • Uniprot No.：
  • 别名：
    Ccnt1; Cyclin-T1; CycT1; Cyclin-T
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-724
  • 氨基酸序列
    MEGERKNNNK RWYFTREQLE NSPSRRFGVD SDKELSYRQQ AANLLQDMGQ RLNVSQLTIN TAIVYMHRFY MIQSFTQFHR YSMAPAALFL AAKVEEQPKK LEHVIKVAHT CLHPQESLPD TRSEAYLQQV QDLVILESII LQTLGFELTI DHPHTHVVKC TQLVRASKDL AQTSYFMATN SLHLTTFSLQ YTPPVVACVC IHLACKWSNW EIPVSTDGKH WWEYVDATVT LELLDELTHE FLQILEKTPS RLKRIRNWRA YQAAMKTKPD DRGADENTSE QTILNMISQT SSDTTIAGLM SMSTASTSAV PSLPSSEESS SSLTSVDMLQ GERWLSSQPP FKLEAAQGHR TSESLALIGV DHSLQQDGSS AFGSQKQASK SVPSAKVSLK EYRAKHAEEL AAQKRQLENM EANVKSQYAY AAQNLLSHDS HSSVILKMPI ESSENPERPF LDKADKSALK MRLPVASGDK AVSSKPEEIK MRIKVHSAGD KHNSIEDSVT KSREHKEKQR THPSNHHHHH NHHSHRHSHL QLPAGPVSKR PSDPKHSSQT STLAHKTYSL SSTLSSSSST RKRGPPEETG AAVFDHPAKI AKSTKSSLNF PFPPLPTMTQ LPGHSSDTSG LPFSQPSCKT RVPHMKLDKG PPGANGHNAT QSIDYQDTVN MLHSLLSAQG VQPTQAPAFE FVHSYGEYMN PRAGAISSRS GTTDKPRPPP LPSEPPPPLP PLPK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II).
  • 基因功能参考文献：
    1. Data show that the minimal 90-amino acid AF9 fragment in MLL-AF9 retains an ability to form higher order complexes with AF4*P-TEFb and with DOT1. PMID: 21896721
    2. Data show that the CDK9 and cyclin T1 subunits of P-TEFb are present in mouse oocytes and preimplantation embryos, and that CDK9 is essential for embryonic genome activation in the mouse. PMID: 21639898
    3. Cyclin T1 oncogenic function and suggest a role for this protein in controlling cell cycle probably via Rb/E2F1 pathway. PMID: 20714219
    4. CDK9 has the intrinsic property to shuttle between nucleus and cytoplasm, and enhanced expression of cyclin T1 promotes its nuclear localization. PMID: 12115727
    5. P-TEFb is a key mediator of Myc activated transcription by stimulating elongation PMID: 12177005
    6. Tat and trans-activation-responsive (TAR) RNA-independent induction of HIV-1 long terminal repeat by this protein requires Sp1. PMID: 12458222
    7. cyclin T1 binds with granulin to inhibit transcription PMID: 12588988
    8. location of the cyclin T1 gene regulatory elements is male germ cell-specific PMID: 12914944
    9. These data link the P-TEFb equilibrium to the intracellular transcriptional demand and proliferative/differentiated states of cells. PMID: 16980611
    10. knockdown of CycT1 from P-TEFb abolishes both of the different chromatin loops regulating CD4 expression PMID: 18039856
    11. the reduced level of CLP-1 introduced in the background of elevated levels of cyclin T1 elevates derepression of P-TEFb activity and emphasizes the importance of the role of CLP-1 in the mechanism governing compensatory hypertrophy in cardiomyocytes. PMID: 19443839

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Cyclin family, Cyclin C subfamily
  • 数据库链接：

    KEGG: mmu:12455

    STRING: 10090.ENSMUSP00000012104

    UniGene: Mm.29941